dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0052401
Uniprot Accession G5E870; TRIPC_MOUSE; Q3TY88; Q3UYT5; Q8BM59; Q8K051;
Genbank Protein ID NP_598736.4;
Genbank Nucleotide ID NM_133975.4;
Protein Name E3 ubiquitin-protein ligase TRIP12
Protein Synonyms/Alias Thyroid receptor-interacting protein 12;TR-interacting protein 12;TRIP-12;
Gene Name Trip12
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)
E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity).
Phosphorylation Sites
dbPAF PTMs: 53 (View all)
PositionPeptidesSourceReferences ( PMIDs )
12PNNNPGGSLRRSQRNcurated25338131
20LRRSQRNTAGAQPQDPhosphoSitePlus22135298
75NSRGVKRSASPDYNRcurated25266776; 23684622; 20469934
77RGVKRSASPDYNRTNPHOSIDA;PhosphoSitePlus;SysPTM 2.0;curated21081558; 22135298; 18846507; 19366988; 25266776; 23684622;
83ASPDYNRTNSPSSAKPhosphoSitePlus;curated22135298; 23684622; 21183079; 25338131
85PDYNRTNSPSSAKKPPhosphoSitePlus;UniProt;curated22135298; 21183079; 25266776; 23684622; 22817900; 25338131
87YNRTNSPSSAKKPRAPhosphoSitePlus;curated22135298; 22817900; 21183079
154QKRKRTESSCVKSGScurated23684622
159TESSCVKSGSGSESTcurated25266776; 23684622
161SSCVKSGSGSESTGAPhosphoSitePlus;curated22135298; 23684622; 20469934
165KSGSGSESTGAEERScurated20469934
196GCSTITDSSSAASTSPhosphoSitePlus22135298
197CSTITDSSSAASTSSPhosphoSitePlus22135298
211SSSSAIASASSTVPAPhosphoSitePlus22135298
245SSPSPRRSSREKEQSPhosphoSitePlus22135298
246SPSPRRSSREKEQSKPhosphoSitePlus22135298
267FDWAARFSPKVSLPKPhosphoSitePlus;curated22135298; 22817900; 21183079
310RKSTKKRSESPPAELPHOSIDA;PhosphoSitePlus;UniProt;curated21081558; 22135298; 21183079; 25266776; 21743459; 22817900;
312STKKRSESPPAELPSPhospho.ELM 9.0;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;UniProt;curated19144319; 21081558; 22135298; 17242355; 19366988; 18056256;
340ASTSRRGSGLGKRGAPhosphoSitePlus22135298
Sequence
(Fasta)
MSNRPNNNPG GSLRRSQRNT AGAQPQDDSI GGRSCSSSSA VIVPQPEDPD RANTSERQKT 60
GQVPKKDNSR GVKRSASPDY NRTNSPSSAK KPRAFQHIES FSETNKPHSK SKKRHLDQEQ 120
QLKSAQLPST SKAHTRKSVA AGSSRNQKRK RTESSCVKSG SGSESTGAEE RSAKPIKLAS 180
KSATSAKAGC STITDSSSAA STSSSSSAIA SASSTVPAGA RVKQGKDQNK ARRSRSASSP 240
SPRRSSREKE QSKTGGSSKF DWAARFSPKV SLPKTKLSLP GSSKSETSKP GPSGLQAKLA 300
SLRKSTKKRS ESPPAELPSL RRSTRQKTTG SCASTSRRGS GLGKRGAAEA RRQEKMADPE 360
SNQETVNSSA ARTDEAPQGA AASSSVAGAV GMTTSGESES DDSEMGRLQA LLEARGLPPH 420
LFGPLGPRMS QLFHRTIGSG ASSKAQQLLQ GLQASDESQQ LQAVIEMCQL LVMGNEETLG 480
GFPVKSVVPA LITLLQMEHN FDIMNHACRA LTYMMEALPR SSAVVVDAIP VFLEKLQVIQ 540
CIDVAEQALT ALEMLSRRHS KAILQAGGLA DCLLYLEFFS INAQRNALAI AANCCQSITP 600
DEFHFVADSL PLLTQRLTHQ DKKSVESTCL CFARLVDNFQ HEENLLQQVA SKDLLTNVQQ 660
LLVVTPPILS SGMFIMVVRM FSLMCSNCPT LAVQLMKQNI AETLHFLLCG ASNGSCQEQI 720
DLVPRSPQEL YELTSLICEL MPCLPKEGIF AVDTMLKKGN AQNTDGAIWQ WRDDRGLWHP 780
YNRIDSRIIE AAHQVGEDEI SLSTLGRVYT IDFNSMQQIN EDTGTARAIQ RKPNPLANSN 840
TSGYSELKKD DARAQLMKED PELAKSFIKT LFGVLYEVYS SSAGPAVRHK CLRAILRIIY 900
FADAELLKDV LKNHAVSSHI ASMLSSQDLK IVVGALQMAE ILMQKLPDIF SVYFRREGVM 960
HQVKHLAESE SLLTSPPKAC TNGSGSLGST TPASSGTATA ATNASADLGS PSLQHSRDDS 1020
LDLSPQGRLS DVLKRKRLPK RGPRRPKYSP PRDDDKVDNQ AKSPTTTQSP KSSFLASLNP 1080
KTWGRLSAQS NSNNIEPART AGVSGLARAA SKDTISNNRE KIKGWIKEQA HKFVERYFSS 1140
ENMDGSNPAL NVLQRLCAAT EQLNLQVDGG AECLVEIRSI VSESDVSSFE IQHSGFVKQL 1200
LLYLTSKNEK DAVGREIRLK RFLHVFFSSP LPGEEPVGRV EPVGHAPLLA LVHKMNNCLS 1260
QMEQFPVKVH DFPSGNGAGG SFSLNRGSQA LKFFNTHQLK CQLQRHPDCA NVKQWKGGPV 1320
KIDPLALVQA IERYLVVRGY GRVREDDEDS DDDGSDEEID ESLAAQFLNS GNVRHRLQFY 1380
IGEHLLPYNM TVYQAVRQFS VQAEDEREST DDESNPLGRA GIWTKTHTIW YKPVREDEES 1440
TKDCVGGKRG RAQTAPTKTS PRNAKKHDEL WHDGVCPSVA NPLEVYLIPT PPENITFEDP 1500
SLDVILLLRV LHAISRYWYY LYDNAMCKEI IPTSEFINSK LTAKANRQLQ DPLVIMTGNI 1560
PTWLTELGKT CPFFFPFDTR QMLFYVTAFD RDRAMQRLLD TNPEINQSDS QDSRVAPRLD 1620
RKKRTVNREE LLKQAESVMQ DLGSSRAMLE IQYENEVGTG LGPTLEFYAL VSQELQRADL 1680
CLWRGEEVTL SNPKGSQEGT KYIQNLQGLF ALPFGRTAKP AHIAKVKMKF RFLGKLMAKA 1740
IMDFRLVDLP LGLPFYKWML RQETSLTSHD LFDIDPVVAR SVYHLEDIVR QKKRLEQDKS 1800
QTKESLQYAL ETLTMNGCSV EDLGLDFTLP GFPNIELKKG GKDIPVTIHN LEEYLRLVIF 1860
WALNEGVCRQ FDSFRDGFES VFPLCHLQYF YPEELDQLLC GSKADTWDAK TLMECCRPDH 1920
GYTHDSRAVK FLFEILSSFD NEQQRLFLQF VTGSPRLPVG GFRSLNPPLT IVRKTFESTE 1980
NPDDFLPSVM TCVNYLKLPD YSSIDIMRDK LLIAAREGQQ SFHLS 2026
Keyword

KW-0007--Acetylation
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0436--Ligase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR000569--HECT_dom
IPR004170--WWE-dom

PROSITE

PS50237--HECT
PS50918--WWE

Pfam

PF00632--HECT
PF02825--WWE

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016874--F:ligase activity
GO:0046966--F:thyroid hormone receptor binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006974--P:cellular response to DNA damage stimulus
GO:0006281--P:DNA repair
GO:0009790--P:embryo development
GO:2000780--P:negative regulation of double-strand break repair
GO:1901315--P:negative regulation of histone H2A K63-linked ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process