dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0052355
Uniprot Accession G3X9K3; BIG1_MOUSE; Q8BKL2;
Genbank Protein ID NP_001095900.1;
Genbank Nucleotide ID NM_001102430.1;
Protein Name Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Protein Synonyms/Alias
Gene Name Arfgef1
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 28 (View all)
PositionPeptidesSourceReferences ( PMIDs )
48LEEIKVETEKQSPPHPHOSIDA21081558
52KVETEKQSPPHGEAKPHOSIDA;PhosphoSitePlus;curated21081558; 22135298; 25266776; 23684622; 25521595; 25338131
234QQQHLLQSPVSHHEPPhosphoSitePlus;curated22135298; 25266776; 22817900; 21183079; 25338131
237HLLQSPVSHHEPESPPhosphoSitePlus;curated22135298; 25266776; 22817900; 21183079; 25338131
243VSHHEPESPHLRYLPPhosphoSitePlus;curated22135298; 25266776; 22817900; 21183079; 25338131
280DKSLQDDTEPENGSDcurated21183079
286DTEPENGSDISSAENPhosphoSitePlus;UniProt;curated22135298; 21183079; 21743459; 22817900; 25338131
289PENGSDISSAENEQTPhosphoSitePlus;UniProt;curated22135298; 21183079; 22817900; 25338131
290ENGSDISSAENEQTEcurated25338131
391SLPDDRLSVSSNDTQcurated25338131
393PDDRLSVSSNDTQESPhosphoSitePlus;SysPTM 2.0;curated22135298; 17242355; 19366988; 21743459; 21183079; 25338131
394DDRLSVSSNDTQESGPhosphoSitePlus;curated22135298; 21743459; 21183079; 25338131
397LSVSSNDTQESGNSSSysPTM 2.017242355; 19366988
659HPETINRYGSLNSLESysPTM 2.017242355; 19366988
661ETINRYGSLNSLESTPhosphoSitePlus;SysPTM 2.0;curated22135298; 17242355; 19366988; 22817900; 21183079; 25338131
664NRYGSLNSLESTSSSPhosphoSitePlus;curated22135298; 21183079
677SSGIGSYSTQMSGTDcurated23567750
681GSYSTQMSGTDNPEQcurated21183079; 25338131
891TKELTIPTKSTKQNVPhosphoSitePlus22135298
1076TVRGREGSLTGTKDQPHOSIDA;PhosphoSitePlus;curated21081558; 22135298; 25266776; 21183079; 25338131
Sequence
(Fasta)		MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK QSPPHGEAKA 60
GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL DCLQKLIAYG HLTGRAPDST 120
TPGKKLIDRI IETICGCFQG PQTDEGVQLQ IIKALLTAVT SQHIEIHEGT VLQAVRTCYN 180
IYLASKNLIN QTTAKATLTQ MLNVIFARME NQALQEAKQM ERERHRQQQH LLQSPVSHHE 240
PESPHLRYLP PQTVDHINQE HEGDLEPQTH DVDKSLQDDT EPENGSDISS AENEQTEADQ 300
ATAAETLSKN DILYDGDYEE KPLDIVQSIV EEMVNIIVGD MGEGMAISAS TEGNTGTVED 360
GSDSENIQAN GIPGTPISVA YTPSLPDDRL SVSSNDTQES GNSSGPSPGA KFSHILQKDA 420
FLVFRSLCKL SMKPLSDGPP DPKSHELRSK ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ 480
YLCVALSKNG VSSVPEVFEL SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD 540
HKWMVIQTLT RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV 600
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEV KHPETINRYG 660
SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE QGIDLFNKKP KRGIQYLQEQ 720
GMLGTTPEDI AQFLHQEERL DSTQVGEFLG DNDKFNKEVM YAYVDQHDFS GKDFVSALRL 780
FLEGFRLPGE AQKIDRLMEK FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV 840
KNKMTKEQYI KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS 900
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW TPFLAAFSVG 960
LQDCDDTEVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL ARFTLLTVSS GITEMKQKNI 1020
DTIKTLITVA HTDGNYLGNS WHEILKCISQ LELAQLIGTG VKPRYISGTV RGREGSLTGT 1080
KDQAPDEFVG LGLVGGNVDW KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF 1140
VRWLCAVSMD ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN 1200
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR DMVVRCIAQM 1260
VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTTGH IVTLVFEKHF PATIDSFQDA 1320
VKCLSEFACN AAFPDTSMEA IRLIRHCAKY VSDRPQAFKE YTSDDMSVAP EDRVWVRGWF 1380
PILFELSCII NRCKLDVRTR GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ 1440
QTEKAEWMTT TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC 1500
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAEPPS PSAVSEKPLD 1560
AISQKSVDIH DSIQPRSSDN RQQAPLVSVS TVSEEVSKVK STAKFPEQKL FAALLIKCVV 1620
QLELIQTIDN IVFFPATSKK EDAENLAAAQ RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL 1680
DCLLESHRFA KAFNSNNEQR TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYMDESR 1740
VSAWEEVQQR LLNVCREALS YFLTLTSESH REAWTNLLLL FLTKVLKISD SRFKAHASFY 1800
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINRQ 1847
Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0333--Golgi apparatus
KW-0344--Guanine-nucleotide releasing factor
KW-0472--Membrane
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
Interpro

IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR032629--DCB_dom
IPR023394--Sec7_alpha_orthog
IPR015403--Sec7_C
IPR000904--Sec7_dom
PROSITE

PS50190--SEC7
Pfam

PF16213--DCB
PF09324--DUF1981
PF01369--Sec7
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005794--C:Golgi apparatus
GO:0000139--C:Golgi membrane
GO:0016363--C:nuclear matrix
GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0048471--C:perinuclear region of cytoplasm
GO:0030532--C:small nuclear ribonucleoprotein complex
GO:0005802--C:trans-Golgi network
GO:0005086--F:ARF guanyl-nucleotide exchange factor activity
GO:0005085--F:guanyl-nucleotide exchange factor activity
GO:0017022--F:myosin binding
GO:0034237--F:protein kinase A regulatory subunit binding
GO:0010256--P:endomembrane system organization
GO:0007030--P:Golgi organization
GO:0030837--P:negative regulation of actin filament polymerization
GO:0034260--P:negative regulation of GTPase activity
GO:0031175--P:neuron projection development
GO:0043547--P:positive regulation of GTPase activity
GO:0090284--P:positive regulation of protein glycosylation in Golgi
GO:0051897--P:positive regulation of protein kinase B signaling
GO:0090303--P:positive regulation of wound healing
GO:0015031--P:protein transport
GO:0032012--P:regulation of ARF protein signal transduction
GO:2000114--P:regulation of establishment of cell polarity
GO:0016192--P:vesicle-mediated transport