dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0050949
Uniprot Accession E9Q7F2; RN169_MOUSE; Q69Z47;
Genbank Protein ID NP_780597.2;
Genbank Nucleotide ID NM_175388.3;
Protein Name E3 ubiquitin-protein ligase RNF169
Protein Synonyms/Alias RING finger protein 169;
Gene Name Rnf169
Gene Synonyms/Alias Kiaa1991;
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
12GPSTRASSAAAAAALPhosphoPep 2.0;curated19060867; 25338131
44GAPGPASSPALLVLRPhosphoSitePlus;curated22135298; 25338131
234EQCPARLSDSENEEPdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 25266776;
236CPARLSDSENEEPSRPhosphoSitePlus;curated22135298; 25266776; 25338131
277LNTKVQRSQSCSDTVcurated25266776
279TKVQRSQSCSDTVQDPhosphoSitePlus;curated22135298; 25266776; 23684622
306RAKITTITPGSTPIIdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 22817900
309ITTITPGSTPIIGVLdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 25266776;
310TTITPGSTPIIGVLLdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 25266776;
326TQNNRCLSAPDLTIEdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298; 25266776; 22817900;
353SLHKPERSISPESNDdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298
358ERSISPESNDSISEEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 22817900
361ISPESNDSISEELNHdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298
390LPDGRVLSPLIIKSTdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated19854140; 20415495; 21183079; 21659605; 16381945; 23193290;
410RSLQKQTSYEASPRIcurated25338131
414KQTSYEASPRILKKWcurated25266776
630VKKLRPPSSDMDLAPPhosphoPep 2.0;curated19060867; 25266776; 25338131
631KKLRPPSSDMDLAPKcurated21183079; 25338131
679TMSRRKGSVDQYLLRdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19854140; 21183079; 16381945; 23193290; 19060867; 22135298;
Sequence
(Fasta)		MAAAGPSTRA SSAAAAAALS RRGRRGRCDE MAAAKAGAPG PASSPALLVL RSAPRPEESG 60
CTGCLETPGE VAALPCSHSR CRGCASRAAG PGCRRCRPRG SGWARRRARD DGQAAAELMG 120
ERARRGQPEP CRPRRDGGAA ASGPRPEPEP LAEPEFIFRT PIKLSKPGEL SEEYGCLRKL 180
RGEKLQEEKD CDDQIHKLLQ EDSEMGKRKA DEQKKRDEAV VLKTSLEQCP ARLSDSENEE 240
PSRGQMMQTH RSAFVSKNSS CSLAFLAGKL NTKVQRSQSC SDTVQDRVRS RLRTAPPNRA 300
KITTITPGST PIIGVLLSTQ NNRCLSAPDL TIEKRLPFGS LSSLASLHKP ERSISPESND 360
SISEELNHFK PIVCSPCTPP KRLPDGRVLS PLIIKSTPRN LTRSLQKQTS YEASPRILKK 420
WEQIFQERQI KKTLSKATLT SLAPEAGEEF PGSDTIHSSK ERPSLAFNTR LSRVQVLSEC 480
AGPTSTALEC FPSVNQTKVE QDCVRKRSRE FSLETCHSSE HGGASSGPSL EREQCEESGS 540
TVDATLVKTC ISTVMKTAAV NSLLPKNDVL GGVLKTKQQL KTLNHFDLGN GILVNSLGEE 600
PIPSLRRGRK RRCKTKHLEQ NGVKKLRPPS SDMDLAPKDP GLLEVGRKLQ QEEEDQQLAL 660
QSHRMFDSER RTMSRRKGSV DQYLLRSSSL AGAK 695
Keyword

KW-0181--Complete proteome
KW-0227--DNA damage
KW-0436--Ligase
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
PROSITE

PS50089--ZF_RING_2
Pfam
Gene Ontology

GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0070530--F:K63-linked polyubiquitin binding
GO:0016874--F:ligase activity
GO:0031491--F:nucleosome binding
GO:0008270--F:zinc ion binding
GO:0006974--P:cellular response to DNA damage stimulus
GO:2000780--P:negative regulation of double-strand break repair
GO:0016567--P:protein ubiquitination