| Tag |
Content |
| dbPAF ID |
dbPAF-0040906 |
| Uniprot Accession |
Q9UUA2; PIF1_SCHPO; O74239;
|
| Genbank Protein ID |
NP_596488.1;
|
| Genbank Nucleotide ID |
NM_001022408.2;
|
| Protein Name |
ATP-dependent DNA helicase pfh1 |
| Protein Synonyms/Alias |
DNA repair and recombination helicase pfh1;PIF1 helicase homolog;RRM3/PIF1 homolog 1; |
| Gene Name |
pfh1 |
| Gene Synonyms/Alias |
pif1;rph1;SPBC887.14c; |
| Organism |
Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast) |
| NCBI Taxa ID |
284812 |
Functional Description
(View all) |
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). Required for both repair of mitochondrial DNA and recognition of a recombinogenic signal characterized by a 26-bp palindromic at sequence in the ery region of mitochondrial DNA. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. Required for the completion of S-phase.Functional Description
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). Required for both repair of mitochondrial DNA and recognition of a recombinogenic signal characterized by a 26-bp palindromic at sequence in the ery region of mitochondrial DNA. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. Required for the completion of S-phase. |
Phosphorylation Sites
|
dbPAF PTMs: 4 |
Sequence
(Fasta) | MFSCQSLYKF SHSFRKRIPV MFQRAQQKSS LLHTQNESSH QPSLNKLGGF SSASLNFNSS 60
RSSTNDDQQT FSSQSDNLPS SPITLPAKRG RSAASLKQLD NTVGFDVSKP SLPVFENSGL 120
GSKYSTEIAN GVYIDENDFD DDLLLENDID QKPIPWSSSP IEHTKLTKSM LSSEKRSKNH 180
LSKIYEDHTS EKGASSVISS NITRQGIKRS RTLPWAVDPY RYGDPDPKRT STSADISQHT 240
VSNDSSNKLS NGRSSSLDSL AKKRMSKSKS TPQISKKFSV PLNSASKSPI GSSLFKTSDS 300
RKKSVPSIFL SDEQKRILDM VVEQQHSIFF TGSAGTGKSV LLRKIIEVLK SKYRKQSDRV 360
AVTASTGLAA CNIGGVTLHS FAGVGLARES VDLLVSKIKK NKKCVNRWLR TRVLIIDEVS 420
MVDAELMDKL EEVARVIRKD SKPFGGIQLV LTGDFFQLPP VPENGKESKF CFESQTWKSA 480
LDFTIGLTHV FRQKDEEFVK MLNELRLGKL SDESVRKFKV LNRTIEYEDG LLPTELFPTR 540
YEVERSNDMR MQQINQNPVT FTAIDSGTVR DKEFRDRLLQ GCMAPATLVL KVNAQVMLIK 600
NIDDQLVNGS LGKVIGFIDD ETYQMEKKDA EMQGRNAFEY DSLDISPFDL PDVKQKKYKL 660
IAMRKASSTA IKWPLVRFKL PNGGERTIVV QRETWNIELP NGEVQASRSQ IPLILAYAIS 720
IHKAQGQTLD RVKVDLGRVF EKGQAYVALS RATTQEGLQV LNFSPAKVMA HPKVVQFYKQ 780
LASVNGLPIR NENKAPVQMR GVKNK
806Fasta Sequence
>Q9UUA2|pfh1|Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
MFSCQSLYKFSHSFRKRIPVMFQRAQQKSSLLHTQNESSHQPSLNKLGGFSSASLNFNSSRSSTNDDQQTFSSQSDNLPSSPITLPAKRGRSAASLKQLDNTVGFDVSKPSLPVFENSGLGSKYSTEIANGVYIDENDFDDDLLLENDIDQKPIPWSSSPIEHTKLTKSMLSSEKRSKNHLSKIYEDHTSEKGASSVISSNITRQGIKRSRTLPWAVDPYRYGDPDPKRTSTSADISQHTVSNDSSNKLSNGRSSSLDSLAKKRMSKSKSTPQISKKFSVPLNSASKSPIGSSLFKTSDSRKKSVPSIFLSDEQKRILDMVVEQQHSIFFTGSAGTGKSVLLRKIIEVLKSKYRKQSDRVAVTASTGLAACNIGGVTLHSFAGVGLARESVDLLVSKIKKNKKCVNRWLRTRVLIIDEVSMVDAELMDKLEEVARVIRKDSKPFGGIQLVLTGDFFQLPPVPENGKESKFCFESQTWKSALDFTIGLTHVFRQKDEEFVKMLNELRLGKLSDESVRKFKVLNRTIEYEDGLLPTELFPTRYEVERSNDMRMQQINQNPVTFTAIDSGTVRDKEFRDRLLQGCMAPATLVLKVNAQVMLIKNIDDQLVNGSLGKVIGFIDDETYQMEKKDAEMQGRNAFEYDSLDISPFDLPDVKQKKYKLIAMRKASSTAIKWPLVRFKLPNGGERTIVVQRETWNIELPNGEVQASRSQIPLILAYAISIHKAQGQTLDRVKVDLGRVFEKGQAYVALSRATTQEGLQVLNFSPAKVMAHPKVVQFYKQLASVNGLPIRNENKAPVQMRGVKNK
|
| Keyword |
KW-0024--Alternative initiation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0233--DNA recombination
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0347--Helicase
KW-0378--Hydrolase
KW-0496--Mitochondrion
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0809--Transit peptide
|
| Interpro |
IPR003593--AAA+_ATPase
IPR003840--DNA_helicase
IPR010285--DNA_helicase_pif1-like
IPR027417--P-loop_NTPase
|
| PROSITE |
|
| Pfam |
PF02689--Herpes_Helicase
PF05970--PIF1
|
| Gene Ontology |
GO:0005739--C:mitochondrion
GO:0000790--C:nuclear chromatin
GO:0005730--C:nucleolus
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0005524--F:ATP binding
GO:0043141--F:ATP-dependent 5'-3' DNA helicase activity
GO:0016887--F:ATPase activity
GO:0003677--F:DNA binding
GO:0017116--F:single-stranded DNA-dependent ATP-dependent DNA helicase activity
GO:0032508--P:DNA duplex unwinding
GO:0006310--P:DNA recombination
GO:1902983--P:DNA strand elongation involved in mitotic DNA replication
GO:0044806--P:G-quadruplex DNA unwinding
GO:0043504--P:mitochondrial DNA repair
GO:1903469--P:removal of RNA primer involved in mitotic DNA replication
GO:0000723--P:telomere maintenance
|
