dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0040800
Uniprot Accession Q9URU2; DNA2_SCHPO; O74241; Q9UTT6; Q9UUK8;
Genbank Protein ID NP_596499.2;
Genbank Nucleotide ID NM_001022420.2;
Protein Name DNA replication ATP-dependent helicase/nuclease dna2
Protein Synonyms/Alias
Gene Name dna2
Gene Synonyms/Alias SPBC16D10.04c;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)
Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for fen1. Is a target of the intra-S phase checkpoint, associating with stalled replication forks when phosphorylated at Ser-219 and preventing the stalled replication forks from reversing. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. Also required for the production of G-rich single-strand overhangs at telomere ends and thus in telomere length maintenance. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is atypical: it cannot load onto its tracking strand internally and has an absolute free 5'-end requirement. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.
Phosphorylation Sites
dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
134KDLCRSSSTQHLLDHUniProt22682245
200VSENQSRSFGSYDEVcurated22817900
Sequence
(Fasta)
MFNDQSKTTS SVKGICATTD NNHGNLKKTN STPFRKNYLL NGRTKLKLEN FAYNASTEIS 60
SPKISEKKHS SLPIKRKNTF NESSTSFSPF TKAHKEITDD LKPDKSFTRK SDLNSQDMPV 120
CFQETSKDLC RSSSTQHLLD HQTTDSTIID MKPVSTNSKS DVFTLYTDET VLLRRCASDN 180
KPLINNNLSS SNVSENQSRS FGSYDEVKNQ GNNLHKVPSL VSIIRNARSS EQSRIAANSS 240
CLLKGSDTEI DEDDFALEAE DLAALDSLER QYSQLPNSTV TASAKDIEKT AKVNHVGGDL 300
QSYCSATKAS DATINEEPVN LALDKACNSL PDINSDFIDD WDDSCDGCTP GELCEFSSEY 360
TVLEVHEDFI FHEGNHFRQL KLILEANDIL HQLFLRGDWT ETSIFVGDSI RVEATFDKDN 420
TAIVDNDKGL IIIHPKILMS ATAVASSFPC LRKAVISDRV GIYGPPTKAM VTGNILHDFF 480
QHALYRGIDA LENVDINLET SIKTYISDIY FADLSLDEIR EELDARLPLL KSIVERYLIS 540
KKNDNNNESI HISRLLDIEE SIWSPRFGLK GNIDATVEVV LTEKPESSST LTLPLELKTG 600
RYVDNISHFA QSLLYTLLIS DRYGINTNQA LLCYLENSTI KNLVASNSQL RGLIMTRNSL 660
AQHNFRRSLP EMISNRKICD HCSLVSECLF FQKMSDKGVA NSNGLTESWN EWMREVKDED 720
LEFYKKWEKL LNQEERLLLL KRGDVLTFDT EELEAYGKTL YPLYITKEDI VCLEIDDRVF 780
HYKFAFLNDN GYPRNFLHSG FSVGERVFIS DEHGHWSLAK GHIVHIQDSC IEVRTRHRLH 840
IPWLKMPNFD FKKNQVFFGN YEDSKLSFIG SNHTRYRIDK DEFSSGIASI RGTLMSSVLP 900
DAPLIIRDMI IRLKPPKFCN SALIDPEFLK CLNEDQITAL KKCHAAEHYS LILGMPGTGK 960
TTTISSLIRS LLAKKKKILL TSFTHLAVDN ILIKLKGCDS TIVRLGSPHK IHPLVKEFCL 1020
TEGTTFDDLA SLKHFYEDPQ IVACSSLGVY HSIFNKRKFD YCIIDEASQI PLPICLGPLQ 1080
LAEKFVLVGD HYQLPPLVKN SRTSKDGLSL SLFKLLSEKH PEAVTTLRLQ YRMNEDINSL 1140
SSELIYGGNL VCGSKTISQK KLILPKAHLS DGLPDSSSSL HWVNKLINPS HSVIFFNTDD 1200
ILGVESKTNN ILENHTEAFL IEQAVSSFLE RGVKQSSIGI ISIYKSQVEL LSKNLKSFTE 1260
IEINTVDRYQ GRDKDIILIS FVRSNSKNLV GELLRDWHRL NVALSRAKVK CIMFGSLSTL 1320
SSSNIVSHLL KLLEKNKWIF TLNENDIATK FDENSSPIKD CSQVATTNNA KVIIRKNQRF 1380
FNSDNLCEKA ILPQLEF 1398
Keyword

KW-0004--4Fe-4S
KW-0067--ATP-binding
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0227--DNA damage
KW-0234--DNA repair
KW-0235--DNA replication
KW-0238--DNA-binding
KW-0255--Endonuclease
KW-0347--Helicase
KW-0378--Hydrolase
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0779--Telomere

Interpro

IPR026851--Dna2
IPR022765--Dna2/Cas4_DUF83
IPR014808--DNA_replication_fac_Dna2_N
IPR027417--P-loop_NTPase

PROSITE
Pfam

PF01930--Cas_Cas4
PF08696--Dna2

Gene Ontology

GO:0000781--C:chromosome, telomeric region
GO:0005829--C:cytosol
GO:0043596--C:nuclear replication fork
GO:0005634--C:nucleus
GO:0005657--C:replication fork
GO:0035861--C:site of double-strand break
GO:0051539--F:4 iron, 4 sulfur cluster binding
GO:1990601--F:5' overhang single-stranded DNA endodeoxyribonuclease activity
GO:0017108--F:5'-flap endonuclease activity
GO:0005524--F:ATP binding
GO:0004003--F:ATP-dependent DNA helicase activity
GO:0003682--F:chromatin binding
GO:0046872--F:metal ion binding
GO:0004518--F:nuclease activity
GO:0019901--F:protein kinase binding
GO:0045145--F:single-stranded DNA 5'-3' exodeoxyribonuclease activity
GO:0043142--F:single-stranded DNA-dependent ATPase activity
GO:0042162--F:telomeric DNA binding
GO:0032508--P:DNA duplex unwinding
GO:0006281--P:DNA repair
GO:0090305--P:nucleic acid phosphodiester bond hydrolysis
GO:1903461--P:Okazaki fragment processing involved in mitotic DNA replication
GO:1903469--P:removal of RNA primer involved in mitotic DNA replication
GO:0031297--P:replication fork processing
GO:0071932--P:replication fork reversal
GO:0072429--P:response to intra-S DNA damage checkpoint signaling
GO:0000723--P:telomere maintenance
GO:0031860--P:telomeric 3' overhang formation