| Tag |
Content |
| dbPAF ID |
dbPAF-0039453 |
| Uniprot Accession |
Q9P7J7; ADA2_SCHPO;
|
| Genbank Protein ID |
NP_588011.1;
|
| Genbank Nucleotide ID |
NM_001023002.2;
|
| Protein Name |
Transcriptional adapter 2 |
| Protein Synonyms/Alias |
|
| Gene Name |
ada2 |
| Gene Synonyms/Alias |
SPCC24B10.08c; |
| Organism |
Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast) |
| NCBI Taxa ID |
284812 |
Functional Description
(View all) |
Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation (gcn5) and deubiquitination (ubp8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B (By similarity). Required for full activation of chromatin alteration and meiotic recombination at meiotic recombination hot spot ade6-M26. Also required for the regulation of transcription and chromatin structure around ade6-M26 in response to osmotic stress. Needed for hyperacetylation of histone H3 around ade6-M26.Functional Description
Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation (gcn5) and deubiquitination (ubp8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B (By similarity). Required for full activation of chromatin alteration and meiotic recombination at meiotic recombination hot spot ade6-M26. Also required for the regulation of transcription and chromatin structure around ade6-M26 in response to osmotic stress. Needed for hyperacetylation of histone H3 around ade6-M26. |
Phosphorylation Sites
|
dbPAF PTMs: 1 | Position | Peptides | Source | References ( PMIDs ) |
|---|
| 330 | KQLRHVKSFNQTTSA | curated | 22817900 |
|
Sequence
(Fasta) | MPPQKYHCNV CAQDITRSIH IRCVECVDFD LCIPCFTSGA SLGTHHPSHP YRIIETNSYP 60
IFDENWGADE ELLLIDACET LGLGNWADIA DYVGNARTKE ECRDHYLKTY IESDCYPLAS 120
VELPGPVDRI AFAARKRARI EAFQPPPIIP QKPLASTPQC HEIQGYMPGR LEFDQEYMNE 180
AELPIKDMNF DDDLHESAKH EMQLKLTMLN IYNSRLTRRA VRKQTIFNHN LLDYRRLQAN 240
EKRMSKEERN LLNKTKAFAR LLTGPDYQKF VNSYHEQITL KKQISDLQEW RQMGLTTLEQ 300
GHKYERDKTQ KFLLSKASAS YDKQLRHVKS FNQTTSAPFQ VRDIQKIVPR KPATPTMFSA 360
SADRQLLSED EQALCSKLQI FPKPFLALKF ALISASLTSK KPFQKTDAVN LFKHLDANKV 420
EQVYDFFHNA RWIGAPT
438Fasta Sequence
>Q9P7J7|ada2|Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
MPPQKYHCNVCAQDITRSIHIRCVECVDFDLCIPCFTSGASLGTHHPSHPYRIIETNSYPIFDENWGADEELLLIDACETLGLGNWADIADYVGNARTKEECRDHYLKTYIESDCYPLASVELPGPVDRIAFAARKRARIEAFQPPPIIPQKPLASTPQCHEIQGYMPGRLEFDQEYMNEAELPIKDMNFDDDLHESAKHEMQLKLTMLNIYNSRLTRRAVRKQTIFNHNLLDYRRLQANEKRMSKEERNLLNKTKAFARLLTGPDYQKFVNSYHEQITLKKQISDLQEWRQMGLTTLEQGHKYERDKTQKFLLSKASASYDKQLRHVKSFNQTTSAPFQVRDIQKIVPRKPATPTMFSASADRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWIGAPT
|
| Keyword |
KW-0002--3D-structure
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0233--DNA recombination
KW-0469--Meiosis
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0346--Stress response
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
KW-0863--Zinc-finger
|
| Interpro |
IPR016827--Ada2/TADA2
IPR009057--Homeodomain-like
IPR001005--SANT/Myb
IPR017884--SANT_dom
IPR007526--SWIRM
IPR000433--Znf_ZZ
|
| PROSITE |
PS51293--SANT
PS50934--SWIRM
PS01357--ZF_ZZ_1
PS50135--ZF_ZZ_2
|
| Pfam |
PF00249--Myb_DNA-binding
PF04433--SWIRM
PF00569--ZZ
|
| Gene Ontology |
GO:0000790--C:nuclear chromatin
GO:0005634--C:nucleus
GO:0000124--C:SAGA complex
GO:0003677--F:DNA binding
GO:0003713--F:transcription coactivator activity
GO:0008270--F:zinc ion binding
GO:0071470--P:cellular response to osmotic stress
GO:0016568--P:chromatin modification
GO:0006338--P:chromatin remodeling
GO:0006310--P:DNA recombination
GO:0016573--P:histone acetylation
GO:0051321--P:meiotic cell cycle
GO:0010520--P:regulation of reciprocal meiotic recombination
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006366--P:transcription from RNA polymerase II promoter
|
