| Tag |
Content |
| dbPAF ID |
dbPAF-0039446 |
| Uniprot Accession |
Q9P7H1; FLP1_SCHPO;
|
| Genbank Protein ID |
NP_594716.1;
|
| Genbank Nucleotide ID |
NM_001020143.2;
|
| Protein Name |
Tyrosine-protein phosphatase CDC14 homolog |
| Protein Synonyms/Alias |
CDC fourteen-like phosphatase 1; |
| Gene Name |
clp1 |
| Gene Synonyms/Alias |
flp1;SPAC1782.09c; |
| Organism |
Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast) |
| NCBI Taxa ID |
284812 |
Functional Description
(View all) |
Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.Functional Description
Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15. |
Phosphorylation Sites
|
dbPAF PTMs: 4 |
Sequence
(Fasta) | MDYQDDGLGE MIEFLEDKLY YTSLSQPPKA ELYPHMHFFT IDDELIYNPF YHDFGPLNVS 60
HLIRFAVIVH GIMGKHRQAK KSKAIVLYSS TDTRLRANAA CLLACYMVLV QNWPPHLALA 120
PLAQAEPPFL GFRDAGYAVS DYYITIQDCV YGLWRARESS ILNIRNIDVH DYETYERVEN 180
GDFNWISPKF IAFASPIQAG WNHASTRPKK LPQPFAIVLD YFVANKVKLI VRLNGPLYDK 240
KTFENVGIRH KEMYFEDGTV PELSLVKEFI DLTEEVEEDG VIAVHCKAGL GRTGCLIGAY 300
LIYKHCFTAN EVIAYMRIMR PGMVVGPQQH WLHINQVHFR AYFYEKAMGR AIQQATAAEP 360
LATPPRHPLN ATNGTSQSNI STPLPEPTPG QPRKVSGHNP PSARRLPSAS SVKFNEKLKN 420
ASKQSIQNEN KASYSSYEDS EIQNDDETRT VGTPTETISV VRLRRSSSQS NIEPNGVRSP 480
TSSPTGSPIR RTSGNRWSSG SSHSKKSAQR SVSMSSLNNT SNGRVAKPKP SKSRLIS
538Fasta Sequence
>Q9P7H1|clp1|Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
MDYQDDGLGEMIEFLEDKLYYTSLSQPPKAELYPHMHFFTIDDELIYNPFYHDFGPLNVSHLIRFAVIVHGIMGKHRQAKKSKAIVLYSSTDTRLRANAACLLACYMVLVQNWPPHLALAPLAQAEPPFLGFRDAGYAVSDYYITIQDCVYGLWRARESSILNIRNIDVHDYETYERVENGDFNWISPKFIAFASPIQAGWNHASTRPKKLPQPFAIVLDYFVANKVKLIVRLNGPLYDKKTFENVGIRHKEMYFEDGTVPELSLVKEFIDLTEEVEEDGVIAVHCKAGLGRTGCLIGAYLIYKHCFTANEVIAYMRIMRPGMVVGPQQHWLHINQVHFRAYFYEKAMGRAIQQATAAEPLATPPRHPLNATNGTSQSNISTPLPEPTPGQPRKVSGHNPPSARRLPSASSVKFNEKLKNASKQSIQNENKASYSSYEDSEIQNDDETRTVGTPTETISVVRLRRSSSQSNIEPNGVRSPTSSPTGSPIRRTSGNRWSSGSSHSKKSAQRSVSMSSLNNTSNGRVAKPKPSKSRLIS
|
| Keyword |
KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0378--Hydrolase
KW-0469--Meiosis
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0904--Protein phosphatase
KW-1185--Reference proteome
KW-0717--Septation
|
| Interpro |
IPR026070--CDC14
IPR029260--DSPn
IPR000340--Dual-sp_phosphatase_cat-dom
IPR029021--Prot-tyrosine_phosphatase-like
IPR016130--Tyr_Pase_AS
IPR000387--TYR_PHOSPHATASE_dom
|
| PROSITE |
PS00383--TYR_PHOSPHATASE_1
PS50056--TYR_PHOSPHATASE_2
|
| Pfam |
PF00782--DSPc
PF14671--DSPn
|
| Gene Ontology |
GO:0005826--C:actomyosin contractile ring
GO:0032153--C:cell division site
GO:0030428--C:cell septum
GO:0000778--C:condensed nuclear chromosome kinetochore
GO:0005737--C:cytoplasm
GO:0000776--C:kinetochore
GO:0072686--C:mitotic spindle
GO:0044732--C:mitotic spindle pole body
GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0005816--C:spindle pole body
GO:0004721--F:phosphoprotein phosphatase activity
GO:0004722--F:protein serine/threonine phosphatase activity
GO:0004725--F:protein tyrosine phosphatase activity
GO:0008138--F:protein tyrosine/serine/threonine phosphatase activity
GO:0000917--P:barrier septum assembly
GO:0006974--P:cellular response to DNA damage stimulus
GO:0035853--P:chromosome passenger complex localization to spindle midzone
GO:0098783--P:correction of merotelic kinetochore attachment, mitotic
GO:0044878--P:cytokinesis checkpoint
GO:0071989--P:establishment of protein localization to spindle pole body
GO:0051321--P:meiotic cell cycle
GO:1902406--P:mitotic actomyosin contractile ring maintenance
GO:0045736--P:negative regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0010972--P:negative regulation of G2/M transition of mitotic cell cycle
GO:0006469--P:negative regulation of protein kinase activity
GO:1902425--P:positive regulation of attachment of mitotic spindle microtubules to kinetochore
GO:0031536--P:positive regulation of exit from mitosis
GO:0031031--P:positive regulation of septation initiation signaling
GO:0006470--P:protein dephosphorylation
GO:0034501--P:protein localization to kinetochore
GO:0007096--P:regulation of exit from mitosis
GO:1902412--P:regulation of mitotic cytokinesis
GO:0033047--P:regulation of mitotic sister chromatid segregation
GO:0072479--P:response to mitotic cell cycle spindle assembly checkpoint signaling
GO:0007034--P:vacuolar transport
|
