dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0034319
Uniprot Accession Q99383; HRP1_YEAST; D6W1U5; Q02741;
Genbank Protein ID NP_014518.1;
Genbank Nucleotide ID NM_001183377.1;
Protein Name Nuclear polyadenylated RNA-binding protein 4
Protein Synonyms/Alias Cleavage factor IB;CFIB;
Gene Name HRP1
Gene Synonyms/Alias NAB4;NAB5;YOL123W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 RNA-binding protein, which is involved in the polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor CFIA complex and the cleavage and polyadenylation factor (CPF) complex. May be involved in regulation of poly(A) site selection. Is involved in nonsense-mediated mRNA decay. Seems to bind to an RNA downstream sequence element (DSE) located 3' of a nonsense codon and may mark the transcript for decay.
Phosphorylation Sites
 dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
2------MSSDEEDFNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 20702584;
3-----MSSDEEDFNDdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 20702584;
18IYGDDKPTTTEEVKKcurated19823750
19YGDDKPTTTEEVKKEcurated22817900; 19823750
34EEQNKAGSGTSQLDQdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
51ALQALSSSLNKLNNPPhosphoGRID 2.0;curated19823750; 19795423; 22369663
60NKLNNPNSNNSSSNNcurated25315811
63NNPNSNNSSSNNSNQcurated25315811
64NPNSNNSSSNNSNQDcurated25315811
65PNSNNSSSNNSNQDTcurated25315811; 20377248
68NNSSSNNSNQDTSSScurated25315811
74NSNQDTSSSKQDGTAcurated25315811
75SNQDTSSSKQDGTANcurated25315811
87TANDKEGSNEDTKNEPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900; 25315811; 20377248
170IGGLNWDTTEDNLREcurated22369663
171GGLNWDTTEDNLREYcurated22369663
184EYFGKYGTVTDLKIMdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 19366988; 22369663;
206SRGFGFLSFEKPSSVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
328RHMQQKSSNNGGNNGcurated25315811
446SDSDNNKSNDVQTIGPhosphoGRID 2.021126336
Sequence
(Fasta)		MSSDEEDFND IYGDDKPTTT EEVKKEEEQN KAGSGTSQLD QLAALQALSS SLNKLNNPNS 60
NNSSSNNSNQ DTSSSKQDGT ANDKEGSNED TKNEKKQESA TSANANANAS SAGPSGLPWE 120
QLQQTMSQFQ QPSSQSPPQQ QVTQTKEERS KADLSKESCK MFIGGLNWDT TEDNLREYFG 180
KYGTVTDLKI MKDPATGRSR GFGFLSFEKP SSVDEVVKTQ HILDGKVIDP KRAIPRDEQD 240
KTGKIFVGGI GPDVRPKEFE EFFSQWGTII DAQLMLDKDT GQSRGFGFVT YDSADAVDRV 300
CQNKFIDFKD RKIEIKRAEP RHMQQKSSNN GGNNGGNNMN RRGGNFGNQG DFNQMYQNPM 360
MGGYNPMMNP QAMTDYYQKM QEYYQQMQKQ TGMDYTQMYQ QQMQQMAMMM PGFAMPPNAM 420
TLNQPQQDSN ATQGSPAPSD SDNNKSNDVQ TIGNTSNTDS GSPPLNLPNG PKGPSQYNDD 480
HNSGYGYNRD RGDRDRNDRD RDYNHRSGGN HRRNGRGGRG GYNRRNNGYH PYNR 535
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0507--mRNA processing
KW-0866--Nonsense-mediated mRNA decay
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0694--RNA-binding
Interpro

IPR012677--Nucleotide-bd_a/b_plait
IPR000504--RRM_dom
PROSITE

PS50102--RRM
Pfam

PF00076--RRM_1
Gene Ontology

GO:0005737--C:cytoplasm
GO:0010494--C:cytoplasmic stress granule
GO:0005849--C:mRNA cleavage factor complex
GO:0005634--C:nucleus
GO:0003729--F:mRNA binding
GO:0000166--F:nucleotide binding
GO:0003723--F:RNA binding
GO:0006378--P:mRNA polyadenylation
GO:0000184--P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0098789--P:pre-mRNA cleavage required for polyadenylation
GO:0072423--P:response to DNA damage checkpoint signaling