dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0034201
Uniprot Accession Q96S44; PRPK_HUMAN; B3KU44; Q3T977; Q5JZ01; Q6NZ60; Q96FM7; Q9NQE6;
Genbank Protein ID NP_291028.3;
Genbank Nucleotide ID NM_033550.3;
Protein Name TP53-regulating kinase
Protein Synonyms/Alias Atypical serine/threonine protein kinase TP53RK;EKC/KEOPS complex subunit TP53RK;
Gene Name TP53RK
Gene Synonyms/Alias C20orf64;PRPK;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Phosphorylation Sites
 dbPAF PTMs: 9
PositionPeptidesSourceReferences ( PMIDs )
7-MAAARATTPADGEEdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated19369195; 16381945; 23193290; 18691976; 18988627; 19060867;
8MAAARATTPADGEEPdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated19369195; 16381945; 23193290; 21659604; 21081558; 19060867;
34ERSSRFLSGLELVKQdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19369195; 16381945; 23193290; 21081558; 19060867; 22135298;
129VRDYIQSTMETEKTPdbPTM 3.0;PhosphoPep 2.0;curated19369195; 16381945; 23193290; 19060867
132YIQSTMETEKTPQGLdbPTM 3.0;PhosphoPep 2.0;curated19369195; 16381945; 23193290; 19060867
135STMETEKTPQGLSNLdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18691976; 19369195; 16381945; 23193290; 18988627; 21081558;
140EKTPQGLSNLAKTIGdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated19369195; 16381945; 23193290; 21081558; 19060867; 22135298;
201EDKGVDLYVLEKAFLdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated19369195; 16381945; 23193290; 21081558; 19060867; 22135298;
250RLRGRKRSMVG----dbPTM 3.0;PhosphoSitePlus17712528; 16381945; 23193290; 22135298
Sequence
(Fasta)		MAAARATTPA DGEEPAPEAE ALAAARERSS RFLSGLELVK QGAEARVFRG RFQGRAAVIK 60
HRFPKGYRHP ALEARLGRRR TVQEARALLR CRRAGISAPV VFFVDYASNC LYMEEIEGSV 120
TVRDYIQSTM ETEKTPQGLS NLAKTIGQVL ARMHDEDLIH GDLTTSNMLL KPPLEQLNIV 180
LIDFGLSFIS ALPEDKGVDL YVLEKAFLST HPNTETVFEA FLKSYSTSSK KARPVLKKLD 240
EVRLRGRKRS MVG 254
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
KW-0819--tRNA processing
Interpro

IPR022495--Bud32
IPR011009--Kinase-like_dom
IPR010440--LipoPS_kinase
IPR000719--Prot_kinase_dom
IPR008266--Tyr_kinase_AS
PROSITE

PS50011--PROTEIN_KINASE_DOM
PS00109--PROTEIN_KINASE_TYR
Pfam

PF06293--Kdo
Gene Ontology

GO:0005829--C:cytosol
GO:0000408--C:EKC/KEOPS complex
GO:0016020--C:membrane
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0016787--F:hydrolase activity
GO:0002039--F:p53 binding
GO:0004674--F:protein serine/threonine kinase activity
GO:0010467--P:gene expression
GO:0006468--P:protein phosphorylation
GO:0006400--P:tRNA modification
GO:0008033--P:tRNA processing
GO:0070525--P:tRNA threonylcarbamoyladenosine metabolic process