dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0032528
Uniprot Accession Q92317; NCB2_YEAST; D6VT30; O00064; P87340; Q04176;
Genbank Protein ID NP_010685.1;
Genbank Nucleotide ID NM_001180705.1;
Protein Name Negative cofactor 2 complex subunit beta
Protein Synonyms/Alias
Gene Name NCB2
Gene Synonyms/Alias YDR1;YDR397C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Component of the NC2 complex which represses RNA polymerase II transcription through binding to SPT15/TBP and thereby inhibiting the assembly of the preinitiation complex. The NC2 complex may also mediate transcriptional activation from TATA-driven promoters through association with SPT15/TBP.
Phosphorylation Sites
 dbPAF PTMs: 7
PositionPeptidesSourceReferences ( PMIDs )
109RDSKFKKSGLSEEELcurated22369663
112KFKKSGLSEEELLRQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;curated18407956; 16381945; 23193290; 12502746; 21081558; 19366988;
135RSRLHHNSVSDPVKSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17330950; 18407956; 16381945; 23193290; 19779198;
137RLHHNSVSDPVKSEDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 16381945; 23193290; 19779198; 19060867; 19366988;
142SVSDPVKSEDSS---dbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 11875433; 19779198;
145DPVKSEDSS------dbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated11875433; 17563356; 18407956; 16381945; 23193290; 17330950;
146PVKSEDSS-------dbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 17330950; 11875433; 20702584;
Sequence
(Fasta)		MAGDSDNVSL PKATVQKMIS EILDQDLMFT KDAREIIINS GIEFIMILSS MASEMADNEA 60
KKTIAPEHVI KALEELEYNE FIPFLEEILL NFKGSQKVKE TRDSKFKKSG LSEEELLRQQ 120
EELFRQSRSR LHHNSVSDPV KSEDSS 147
Keyword

KW-0010--Activator
KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR003958--CBFA_NFYB_domain
IPR009072--Histone-fold
PROSITE
Pfam

PF00808--CBFD_NFYB_HMF
Gene Ontology

GO:0017054--C:negative cofactor 2 complex
GO:0005634--C:nucleus
GO:0003682--F:chromatin binding
GO:0001047--F:core promoter binding
GO:0001129--F:RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly
GO:0003714--F:transcription corepressor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:1900446--P:negative regulation of tRNA transcription from RNA polymerase III promoter
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0061408--P:positive regulation of transcription from RNA polymerase II promoter in response to heat stress
GO:0045898--P:regulation of RNA polymerase II transcriptional preinitiation complex assembly
GO:0051123--P:RNA polymerase II transcriptional preinitiation complex assembly