dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016728
Uniprot Accession Q12680; GLT1_YEAST; D6VRI0; Q12290;
Genbank Protein ID NP_010110.1;
Genbank Nucleotide ID NM_001180231.1;
Protein Name Glutamate synthase [NADH]
Protein Synonyms/Alias NADH-GOGAT;
Gene Name GLT1
Gene Synonyms/Alias YDL171C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.
Phosphorylation Sites
 dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
612CDRLLLKSPILHWNEcurated22369663
857KSVNLPESGEYHFRDcurated22817900; 25315811
977GGEDAERSAVQENGDcurated25315811
1267KAINIDLSPILTPAHcurated22369663; 24961812
1578EYLKKFRSNQEVDDEcurated22817900; 24961812
1588EVDDEVNTLLIANQKcurated22817900; 19547744
1604KEQEKKKSITISNKAPhosphoGRID 2.0;curated19823750; 19795423; 22817900; 25315811
1608KKKSITISNKATLKEcurated19823750
1814TVTVYERSDRCGGLLPhosphoGRID 2.019779198
1860TEIGKTISMDELKNKcurated22817900; 19823750
1947SVRHGAASVLNFELLcurated22369663
2070VKKTRRGTIATLDDSPhosphoGRID 2.0;UniProt;curated19779198; 22817900
Sequence
(Fasta)		MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE HDACGVGFVA 60
NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL GIPHEFMKRE FKLDLDLDIP 120
EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF EDLAASFNLS VLGWRNVPVD STILGDVALS 180
REPTILQPLL VPLYDEKQPE FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK 240
GQLTPAQVYN YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN 300
KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL SLPEAVMMMV 360
PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC GAILDRNGLR PCRYYITSDD 420
RVICASEVGV IPIENSLVVQ KGKLKPGDLF LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS 480
KVIKLDDLLS KTANLVPKEF ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA 540
LGSMGNDAPL ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM 600
HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG YTDTIDKITK 660
LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL IRNKQRSQVA LILETGEARE 720
IHHFCVLLGY GCDGVYPYLA METLVRMNRE GLLRNVNNDN DTLEEGQILE NYKHAIDAGI 780
LKVMSKMGIS TLASYKGAQI FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER 840
GYPSRQTISK SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME 900
AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA HSTLAIAMNR 960
LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV TSYYLSDADE IQIKIAQGAK 1020
PGEGGELPAH KVSKDIAKTR HSTPNVGLIS PPPHHDIYSI EDLKQLIYDL KCANPRAGIS 1080
VKLVSEVGVG IVASGVAKAK ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV 1140
LNDLRRNVVV QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI 1200
ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK LKKRDDVNAK 1260
AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL IDEAEVTLDR GLPVNIDASI 1320
INTDRALGST LSYRVSKKFG EDGLPKDTVV VNIEGSAGQS FGAFLASGIT FILNGDANDY 1380
VGKGLSGGII VIKPPKDSKF KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI 1440
VVERIKGNNA FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL 1500
ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY KKVLLKEKAE 1560
AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE KKKSITISNK ATLKEPKVVD 1620
LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR HETHRDPRTR VNDWKEFTNP ITKKDAKYQT 1680
ARCMDCGTPF CLSDTGCPLS NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC 1740
EGACTLGIIE DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM 1800
LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV TNTEIGKTIS 1860
MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL ESNTKALLNK DLEIIREKIQ 1920
GKKVIVVGGG DTGNDCLGTS VRHGAASVLN FELLPEPPVE RAKDNPWPQW PRVMRVDYGH 1980
AEVKEHYGRD PREYCILSKE FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE 2040
ADIILLSMGF VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV 2100
WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV 2146
Keyword

KW-0003--3Fe-4S
KW-0028--Amino-acid biosynthesis
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0274--FAD
KW-0285--Flavoprotein
KW-0288--FMN
KW-0314--Glutamate biosynthesis
KW-0315--Glutamine amidotransferase
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0479--Metal-binding
KW-0520--NAD
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0865--Zymogen
Interpro

IPR013785--Aldolase_TIM
IPR028261--DPD_II
IPR023753--FAD/NAD-binding_dom
IPR017932--GATase_2_dom
IPR002489--Glu_synth_asu_C
IPR006982--Glu_synth_centr_N
IPR012220--Glu_synth_euk
IPR002932--Glu_synthdom
IPR006005--Glut_synth_ssu1
IPR009051--Helical_ferredxn
IPR016040--NAD(P)-bd_dom
IPR029055--Ntn_hydrolases_N
PROSITE

PS51278--GATASE_TYPE_2
Pfam

PF14691--Fer4_20
PF00310--GATase_2
PF04898--Glu_syn_central
PF01645--Glu_synthase
PF01493--GXGXG
PF07992--Pyr_redox_2
Gene Ontology

GO:0005737--C:cytoplasm
GO:0051538--F:3 iron, 4 sulfur cluster binding
GO:0050660--F:flavin adenine dinucleotide binding
GO:0010181--F:FMN binding
GO:0016040--F:glutamate synthase (NADH) activity
GO:0005506--F:iron ion binding
GO:0019676--P:ammonia assimilation cycle
GO:0006537--P:glutamate biosynthetic process
GO:0097054--P:L-glutamate biosynthetic process