dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016727
Uniprot Accession Q12675; ATC4_YEAST; D6VS80;
Genbank Protein ID NP_010378.1;
Genbank Nucleotide ID NM_001180401.1;
Protein Name Phospholipid-transporting ATPase DNF2
Protein Synonyms/Alias Flippase DNF2;
Gene Name DNF2
Gene Synonyms/Alias YDR093W;YD8557.01;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids. Required for protein transport from Golgi to vacuoles.
Phosphorylation Sites
 dbPAF PTMs: 29 (View all)
PositionPeptidesSourceReferences ( PMIDs )
43FQFEKPSSAHGNIEVcurated25315811
69SKPMKDISTPDLSKVPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19779198; 19795423; 19060867; 26040289; 22369663;
70KPMKDISTPDLSKVTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 16381945; 23193290; 19779198; 19060867; 19366988;
84TFDGIDDYSNDNDINcurated22369663
85FDGIDDYSNDNDINDPhosphoGRID 2.0;PHOSIDA;UniProt;curated19823750; 19779198; 19795423; 21081558; 22369663; 22817900;
386RSFGPRGSLDSIGSYdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 20702584; 19779198; 19366988;
389GPRGSLDSIGSYRMSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 16381945; 23193290; 20702584; 19779198; 19060867;
392GSLDSIGSYRMSADFdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 19779198; 19060867;
393SLDSIGSYRMSADFGPhosphoGRID 2.019779198
396SIGSYRMSADFGRPSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 17330950; 19779198; 19366988;
403SADFGRPSLDYENLNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040289;
406FGRPSLDYENLNQTMPhosphoGRID 2.0;UniProt;curated19779198; 26040289; 22817900
585INAGVTPTKKSRISRPhosphoGRID 2.0;curated19823750; 19795423; 22817900
770EIAKDRETMIDELRSPhosphoGRID 2.019779198
777TMIDELRSMSDNTQFPhosphoGRID 2.0;curated19779198; 22817900; 19547744
782LRSMSDNTQFCPEDLPhosphoGRID 2.0;UniProt;curated19779198; 22817900
1316NQTKFLWYMLDGVYQcurated24961812
1322WYMLDGVYQSVICFFcurated24961812
1324MLDGVYQSVICFFFPcurated24961812
1332VICFFFPYLAYHKNMcurated24961812
Sequence
(Fasta)		MSSPSKPTSP FVDDIEHESG SASNGLSSMS PFDDSFQFEK PSSAHGNIEV AKTGGSVLKR 60
QSKPMKDIST PDLSKVTFDG IDDYSNDNDI NDDDELNGKK TEIHEHENEV DDDLHSFQAT 120
PMPNTGGFED VELDNNEGSN NDSQADHKLK RVRFGTRRNK SGRIDINRSK TLKWAKKNFH 180
NAIDEFSTKE DSLENSALQN RSDELRTVYY NLPLPEDMLD EDGLPLAVYP RNKIRTTKYT 240
PLTFFPKNIL FQFHNFANIY FLILLILGAF QIFGVTNPGF ASVPLIVIVI ITAIKDGIED 300
SRRTVLDLEV NNTRTHILSG VKNENVAVDN VSLWRRFKKA NTRALIKIFE YFSENLTAAG 360
REKKLQKKRE ELRRKRNSRS FGPRGSLDSI GSYRMSADFG RPSLDYENLN QTMSQANRYN 420
DGENLVDRTL QPNPECRFAK DYWKNVKVGD IVRVHNNDEI PADMILLSTS DVDGACYVET 480
KNLDGETNLK VRQSLKCSKI IKSSRDITRT KFWVESEGPH ANLYSYQGNF KWQDTQNGNI 540
RNEPVNINNL LLRGCTLRNT KWAMGMVIFT GDDTKIMINA GVTPTKKSRI SRELNFSVIL 600
NFVLLFILCF TAGIVNGVYY KQKPRSRDYF EFGTIGGSAS TNGFVSFWVA VILYQSLVPI 660
SLYISVEIIK TAQAIFIYTD VLLYNAKLDY PCTPKSWNIS DDLGQIEYIF SDKTGTLTQN 720
VMEFKKCTIN GVSYGRAYTE ALAGLRKRQG VDVESEGRRE KEEIAKDRET MIDELRSMSD 780
NTQFCPEDLT FVSKEIVEDL KGSSGDHQQK CCEHFLLALA LCHSVLVEPN KDDPKKLDIK 840
AQSPDESALV STARQLGYSF VGSSKSGLIV EIQGVQKEFQ VLNVLEFNSS RKRMSCIIKI 900
PGSTPKDEPK ALLICKGADS VIYSRLDRTQ NDATLLEKTA LHLEEYATEG LRTLCLAQRE 960
LTWSEYERWV KTYDVAAASV TNREEELDKV TDVIERELIL LGGTAIEDRL QDGVPDSIAL 1020
LAEAGIKLWV LTGDKVETAI NIGFSCNVLN NDMELLVVKA SGEDVEEFGS DPIQVVNNLV 1080
TKYLREKFGM SGSEEELKEA KREHGLPQGN FAVIIDGDAL KVALNGEEMR RKFLLLCKNC 1140
KAVLCCRVSP AQKAAVVKLV KKTLDVMTLA IGDGSNDVAM IQSADVGVGI AGEEGRQAVM 1200
CSDYAIGQFR YVTRLVLVHG KWCYKRLAEM IPQFFYKNVI FTLSLFWYGI YNNFDGSYLF 1260
EYTYLTFYNL AFTSVPVILL AVLDQDVSDT VSMLVPQLYR VGILRKEWNQ TKFLWYMLDG 1320
VYQSVICFFF PYLAYHKNMV VTENGLGLDH RYFVGVFVTA IAVTSCNFYV FMEQYRWDWF 1380
CGLFICLSLA VFYGWTGIWT SSSSSNEFYK GAARVFAQPA YWAVLFVGVL FCLLPRFTID 1440
CIRKIFYPKD IEIVREMWLR GDFDLYPQGY DPTDPSRPRI NEIRPLTDFK EPISLDTHFD 1500
GVSHSQETIV TEEIPMSILN GEQGSRKGYR VSTTLERRDQ LSPVTTTNNL PRRSMASARG 1560
NKLRTSLDRT REEMLANHQL DTRYSVERAR ASLDLPGINH AETLLSQRSR DR 1613
Keyword

KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0445--Lipid transport
KW-0460--Magnesium
KW-0472--Membrane
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0813--Transport
Interpro

IPR023299--ATPase_P-typ_cyto_domN
IPR018303--ATPase_P-typ_P_site
IPR008250--ATPase_P-typ_transduc_dom_A
IPR023214--HAD-like_dom
IPR006539--P-type_ATPase_IV
IPR032631--P-type_ATPase_N
IPR001757--P_typ_ATPase
IPR032630--P_typ_ATPase_c
IPR026871--PLip_transp_ATPase
PROSITE

PS00154--ATPASE_E1_E2
Pfam

PF00122--E1-E2_ATPase
PF16212--PhoLip_ATPase_C
PF16209--PhoLip_ATPase_N
Gene Ontology

GO:0005783--C:endoplasmic reticulum
GO:0016021--C:integral component of membrane
GO:0070867--C:mating projection tip membrane
GO:0005886--C:plasma membrane
GO:0005524--F:ATP binding
GO:0000287--F:magnesium ion binding
GO:0004012--F:phospholipid-translocating ATPase activity
GO:0006897--P:endocytosis
GO:0007163--P:establishment or maintenance of cell polarity
GO:0006886--P:intracellular protein transport
GO:0045332--P:phospholipid translocation
GO:0000749--P:response to pheromone involved in conjugation with cellular fusion