dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016725
Uniprot Accession Q12532; RQC2_YEAST; D6W403;
Genbank Protein ID NP_015316.1;
Genbank Nucleotide ID NM_001183823.1;
Protein Name Ribosome quality control complex subunit 2
Protein Synonyms/Alias Translation-associated element 2;
Gene Name RQC2
Gene Synonyms/Alias TAE2;YPL009C;YP8132.04C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:23178123). RQC2 is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety (PubMed:25349383). RQC2 is important for the stable association of RKR1/LTN1 to the complex (PubMed:23479637). RQC2 recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal Ala and Thr extensions (CAT tails). CAT tails induce a HSF1-dependent heat shock response (PubMed:25554787).
Phosphorylation Sites
 dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
561LKKKLKDSHSVLKKIcurated22817900
563KKLKDSHSVLKKIRTcurated22817900; 25315811
570SVLKKIRTPYFFEKYcurated25315811
760QQQDEDDSNEVNGLEPhosphoGRID 2.0;curated21126336; 25315811
772GLEKGGDSNDSTKNNPhosphoGRID 2.0;curated19779198; 22817900
775KGGDSNDSTKNNSFEcurated26040289
780NDSTKNNSFEHDNLEPhosphoGRID 2.0;PHOSIDA;curated21177495; 19823750; 19779198; 19795423; 21081558; 26040289;
795KDIEKHCTISSDTDSPhosphoPep 2.0;curated19060867; 25315811
797IEKHCTISSDTDSDSPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated21177495; 20702584; 19779198; 19060867; 22817900; 19823750
798EKHCTISSDTDSDSGdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 20702584; 19779198;
800HCTISSDTDSDSGNAPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated21177495; 19779198; 21081558; 19060867; 26040289; 22817900
802TISSDTDSDSGNAKAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 20702584;
Sequence
(Fasta)		MKQRISALDL LLLARELKQD LEGYRLSNIY NIADSSKQFL LKFNKPDSKL NVVVDCGLRI 60
YLTEFSRPIP PTPSGFVVKL RKHLKAKRLT ALKQVDQDRI LVLQFADGHF YLVLEFFSAG 120
NVILLDENRR IMALQRVVLE HENKVGQIYE MFDESLFTTN NESADESIEK NRKAEYTSEL 180
VNEWIKAVQA KYESDITVIK QLNIQGKEGA KKKKVKVPSI HKLLLSKVPH LSSDLLSKNL 240
KVFNIDPSES CLNLLEETDS LAELLNSTQL EYNQLLTTTD RKGYILAKRN ENYISEKDTA 300
DLEFIYDTFH PFKPYINGGD TDSSCIIEVE GPYNRTLDKF FSTIESSKYA LRIQNQESQA 360
QKKIDDARAE NDRKIQALLD VQELNERKGH LIIENAPLIE EVKLAVQGLI DQQMDWNTIE 420
KLIKSEQKKG NRIAQLLNLP LNLKQNKISV KLDLSSKELN TSSDEDNESE GNTTDSSSDS 480
DSEDMESSKE RSTKSMKRKS NEKINVTIDL GLSAYANATE YFNIKKTSAQ KQKKVEKNVG 540
KAMKNIEVKI DQQLKKKLKD SHSVLKKIRT PYFFEKYSWF ISSEGFLVMM GKSPAETDQI 600
YSKYIEDDDI YMSNSFNSHV WIKNPEKTEV PPNTLMQAGI LCMSSSEAWS KKISSSPWWC 660
FAKNVSKFDG SDNSILPEGA FRLKNENDQN HLPPAQLVMG FGFLWKVKTS GNEDNGDDDE 720
EEEEEEEEEE EEEEEEEEEE EEEKEEEEKE EEQQQDEDDS NEVNGLEKGG DSNDSTKNNS 780
FEHDNLEKDI EKHCTISSDT DSDSGNAKAK NDNSSTQRIL DEPGVPISLI ENINSNVRGK 840
RGKLKKIQKK YADQDETERL LRLEALGTLK GIEKQQQRKK EEIMKREVRE DRKNKREKQR 900
RLQALKFTKK EKARVNYDKH KSELKPSLDK GDVVDDIIPV FAPWPALLKY KYKVKIQPGS 960
AKKTKTLTEI LHYFKSRPLD GSSTDNEMDW PQEHEMIKGL KEQDLVLLLC VDKLKVTIAG 1020
QKSTKNGGNS SKKGKKKR 1039
Keyword

KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
Interpro

IPR021846--DUF3441
IPR008532--DUF814
PROSITE
Pfam

PF11923--DUF3441
PF05670--DUF814
Gene Ontology

GO:0010494--C:cytoplasmic stress granule
GO:1990112--C:RQC complex
GO:0043023--F:ribosomal large subunit binding
GO:0000049--F:tRNA binding
GO:0043043--P:peptide biosynthetic process
GO:1990116--P:ribosome-associated ubiquitin-dependent protein catabolic process
GO:0006412--P:translation