dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016719
Uniprot Accession Q12518; ENT1_YEAST; D6VRI8;
Genbank Protein ID NP_010120.1;
Genbank Nucleotide ID NM_001180221.1;
Protein Name Epsin-1
Protein Synonyms/Alias
Gene Name ENT1
Gene Synonyms/Alias YDL161W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.
Phosphorylation Sites
 dbPAF PTMs: 28 (View all)
PositionPeptidesSourceReferences ( PMIDs )
18NLVKGYSSTQVLVRNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
160KGRRRRGTGRSDENDdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 20702584; 19684113;
163RRRGTGRSDENDDDLdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 20702584;
175DDLQRAISASRLTAEPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 26040406; 25704821; 22369663; 22817900
177LQRAISASRLTAEEDcurated25704821; 22369663
180AISASRLTAEEDERRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040406;
201YETALQLSKEEEELKcurated25315811
311SNNPFAKSEQSSSSPPhosphoPep 2.019060867
314PFAKSEQSSSSPKRNPhosphoPep 2.019060867
315FAKSEQSSSSPKRNQcurated22817900
316AKSEQSSSSPKRNQLPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900; 19823750
317KSEQSSSSPKRNQLVPhosphoPep 2.0;curated19060867; 22817900
327RNQLVAASSPQQLQQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 21126336; 20702584;
328NQLVAASSPQQLQQQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 21177495; 20702584;
346EPLIQNRTGNQSMTDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 17563356; 18407956; 16381945; 23193290; 19823750;
350QNRTGNQSMTDKYSKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 20702584; 19823750; 19795423;
352RTGNQSMTDKYSKLNPhosphoPep 2.0;curated19060867; 22369663
364KLNELLATGTGIDTFdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19823750; 19779198; 19795423;
366NELLATGTGIDTFGNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 19779198; 13679512;
370ATGTGIDTFGNVGEAcurated25521595
Sequence
(Fasta)		MSKQFVRSAK NLVKGYSSTQ VLVRNATSND NHQVSKDSLI ELAEKSYDSA DFFEIMDMLD 60
KRLNDKGKYW RHIAKALTVI DYLIRFGSEN CVLWCRENLY IIKTLKEFRH EDDEGIDQGQ 120
IVRVKAKELT ALLSDDERLN EERNMNIKGR NRKGRRRRGT GRSDENDDDL QRAISASRLT 180
AEEDERRRKQ DEDYETALQL SKEEEELKRL QDLQRMQQQQ GQQQLQQPMY YDIFGNPITP 240
EEYAQFQLQQ QQQQQQQQLQ QQPMYYDVFG NPITPEELAQ FQQQQQLQEQ QYLASMQQQQ 300
QAMSNNPFAK SEQSSSSPKR NQLVAASSPQ QLQQQKQQEP LIQNRTGNQS MTDKYSKLNE 360
LLATGTGIDT FGNVGEARIP AQHTKTGTFI NSQGTGYRQV SDDPNHNPFL NSQYTGLPST 420
SVVPTQTGYG FGNQSQQQSQ NNGSNNRGYT LIDL 455
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0254--Endocytosis
KW-0446--Lipid-binding
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
Interpro

IPR013809--ENTH
IPR008942--ENTH_VHS
IPR003903--UIM_dom
PROSITE

PS50942--ENTH
PS50330--UIM
Pfam

PF01417--ENTH
Gene Ontology

GO:0005769--C:early endosome
GO:0016020--C:membrane
GO:0030276--F:clathrin binding
GO:0008289--F:lipid binding
GO:0043130--F:ubiquitin binding
GO:0000147--P:actin cortical patch assembly
GO:0007015--P:actin filament organization
GO:0006897--P:endocytosis