dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016715
Uniprot Accession Q12514; NOT5_YEAST; D6W476;
Genbank Protein ID NP_015397.1;
Genbank Nucleotide ID NM_001184169.1;
Protein Name General negative regulator of transcription subunit 5
Protein Synonyms/Alias
Gene Name NOT5
Gene Synonyms/Alias YPR072W;YP9499.27;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.
Phosphorylation Sites
 dbPAF PTMs: 31 (View all)
PositionPeptidesSourceReferences ( PMIDs )
102SVEKLMKTKQFSKEAcurated22817900
231PKEGNNQTSLSSIRScurated20377248
232KEGNNQTSLSSIRSScurated22369663
234GNNQTSLSSIRSSKKcurated19823750
235NNQTSLSSIRSSKKQcurated22369663; 19823750; 25315811
238TSLSSIRSSKKQERScurated19823750
239SLSSIRSSKKQERSPcurated19823750
245SSKKQERSPKKKAPQPhosphoGRID 2.0;curated19823750; 19795423; 22817900
256KAPQRDVSISDRATTPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 21126336; 20702584; 19779198; 19060867; 26040289;
258PQRDVSISDRATTPIcurated19823750
262VSISDRATTPIAPGVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19779198; 19366988;
263SISDRATTPIAPGVEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 17330950; 19779198;
271PIAPGVESASQSISSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 19779198; 19060867; 19366988;
273APGVESASQSISSTPPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 22369663; 24961812
275GVESASQSISSTPTPdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 17330950; 19779198; 19366988;
277ESASQSISSTPTPVSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 21081558; 19060867;
278SASQSISSTPTPVSTPhosphoGRID 2.0;curated19779198; 26040289; 19823750; 25315811; 20377248
279ASQSISSTPTPVSTDPhosphoGRID 2.0;PHOSIDA;curated19779198; 21081558; 22369663; 22817900; 19823750; 19795423;
281QSISSTPTPVSTDTPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 19779198; 19060867; 19366988;
291STDTPLHTVKDDSIKcurated23825934
Sequence
(Fasta)		MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 60
DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 120
ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 180
VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 240
KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 300
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 360
YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 420
PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 480
FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 540
WLARRCGNDF VYNEEDFEKL 561
Keyword

KW-0002--3D-structure
KW-0010--Activator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR007282--NOT
IPR007207--Not_N
PROSITE
Pfam

PF04153--NOT2_3_5
PF04065--Not3
Gene Ontology

GO:0030015--C:CCR4-NOT core complex
GO:0005737--C:cytoplasm
GO:0000932--C:cytoplasmic mRNA processing body
GO:0005634--C:nucleus
GO:0031087--P:deadenylation-independent decapping of nuclear-transcribed mRNA
GO:0017148--P:negative regulation of translation
GO:0000289--P:nuclear-transcribed mRNA poly(A) tail shortening
GO:0032968--P:positive regulation of transcription elongation from RNA polymerase II promoter
GO:0016567--P:protein ubiquitination
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006368--P:transcription elongation from RNA polymerase II promoter