dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0016707
Uniprot Accession Q12501; YO22B_YEAST; D6W338;
Genbank Protein ID NP_620389.3;
Genbank Nucleotide ID NM_001184390.4;
Protein Name Transposon Ty2-OR2 Gag-Pol polyprotein
Protein Synonyms/Alias TY2A-TY2B;Transposon Ty2 TYA-TYB polyprotein;
Gene Name TY2B-OR2
Gene Synonyms/Alias YORWTy2-2 POL;YOR343W-B;O6304;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty2 RNA and initiation of reverse transcription (By similarity).
Phosphorylation Sites
dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
1093RSSNSDSTTPIISIEPhosphoPep 2.019060867
1094SSNSDSTTPIISIETPhosphoGRID 2.0;PhosphoPep 2.017330950; 19060867
Sequence
(Fasta)
MESQQLHQNP HCPHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV NSQEETTPGT 60
SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN WAHYQQPSMM TCSHYQTSPA 120
YYQPDPHYPL PQYIPPLSTS SPDPIDSQDQ HSEVPQAKTK VRNNVLPPHP HTSEENFSTW 180
VKFYIRFLKN SNLGDIIPND QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN 240
YSDILTVLCK SVSKMQTNNQ ELKDWIALAN LEYNGSTSAD TFEITVSTII QRLKENNINV 300
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN LNKPSQYKQH 360
SEYKNVSRTS PNTTNTKVTT RNYHRTNSSK PRAAKAHNIA TSSKFSRVNN DHINESTVSS 420
QYLSDDNELS LGQQQKESKP TRTIDSNDEL PDHLLIDSGA SQTLVRSAHY LHHATPNSEI 480
NIVDAQKQDI PINAIGNLHF NFQNGTKTSI KALHTPNIAY DLLSLSELTN QNITACFTRN 540
TLERSDGTVL APIVKHGDFY WLSKKYLIPS HISKLTINNV NKSKSVNKYP YPLIHRMLGH 600
ANFRSIQKSL KKNAVTYLKE SDIEWSNAST YQCPDCLIGK STKHRHVKGS RLKYQESYEP 660
FQYLHTDIFG PVHHLPKSAP SYFISFTDEK TRFQWVYPLH DRREESILNV FTSILAFIKN 720
QFNARVLVIQ MDRGSEYTNK TLHKFFTNRG ITACYTTTAD SRAHGVAERL NRTLLNDCRT 780
LLHCSGLPNH LWFSAVEFST IIRNSLVSPK NDKSARQHAG LAGLDITTIL PFGQPVIVNN 840
HNPDSKIHPR GIPGYALHPS RNSYGYIIYL PSLKKTVDTT NYVILQNNQT KLDQFDYDTL 900
TFDDDLNRLT AHNQSFIEQN ETEQSYDQNT ESDHDYQSEI EINSDPLVND FSSQSLNPLQ 960
LDKEPVQKVR APKEVDADIS EYNILPSTIR SRTPHIINKE STEMGGTIES DTTSPRHSST 1020
FTARNQKRPG SPNDMIDLTS QDRVNYGLEN IKTTRLGGTE EPYIQRNSDT NIKYRTTNST 1080
PSIDDRSSNS DSTTPIISIE TKAACDNTPS IDTDPPEYRS SDHATPNIMP DKSSKNVTAD 1140
SILDDLPLPD LTHKSPTDTS DVSKDIPHIH SRQTNSSLGG MDDSNVLTTT KSKKRSLEDN 1200
ETEIEVSRDT WNNKNMRSLE PPRSKKRINL IAAIKGVKSI KPVRTTLRYD EAITYNKDNK 1260
EKDRYVEAYH KEISQLLKMN TWDTNKYYDR NDIDPKKVIN SMFIFNKKRD GTHKARFVAR 1320
GDIQHPDTYD SDMQSNTVHH YALMTSLSIA LDNDYYITQL DISSAYLYAD IKEELYIRPP 1380
PHLGLNDKLL RLRKSLYGLK QSGANWYETI KSYLINCCDM QEVRGWSCVF KNSQVTICLF 1440
VDDMILFSKD LNANKKIITT LKKQYDTKII NLGEGDNEIQ YDILGLEIKY QRSKYMKLGM 1500
EKSLTEKLPK LNVPLNPKGK KLRAPGQPGH YIDQDELEID EDEYKEKVHE MQKLIGLASY 1560
VGYKFRFDLL YYINTLAQHI LFPSRQVLDM TYELIQFMWD TRDKQLIWHK NKPTKPDNKL 1620
VAISDASYGN QPYYKSQIGN IFLLNGKVIG GKSTKASLTC TSTTEAEIHA VSEAIPLLNN 1680
LSHLVQELNK KPIIKGLLTD SRSTISIIKS TNEEKFRNRF FGTKAMRLRD EVSGNNLYVY 1740
YIETKKNIAD VMTKPLPIKT FKLLTNKWIH 1771
Keyword

KW-0064--Aspartyl protease
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0229--DNA integration
KW-0233--DNA recombination
KW-0238--DNA-binding
KW-0239--DNA-directed DNA polymerase
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0688--Ribosomal frameshifting
KW-0694--RNA-binding
KW-0695--RNA-directed DNA polymerase
KW-0808--Transferase
KW-0814--Transposable element
KW-0815--Transposition
KW-0917--Virion maturation
KW-1188--Virus exit from host cell
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR025724--GAG-pre-integrase_dom
IPR001584--Integrase_cat-core
IPR015820--Retrotransposon_Ty1A_N
IPR012337--RNaseH-like_dom
IPR013103--RVT_2

PROSITE

PS50994--INTEGRASE

Pfam

PF13976--gag_pre-integrs
PF00665--rve
PF07727--RVT_2
PF01021--TYA

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0000943--C:retrotransposon nucleocapsid
GO:0004190--F:aspartic-type endopeptidase activity
GO:0005524--F:ATP binding
GO:0003677--F:DNA binding
GO:0003887--F:DNA-directed DNA polymerase activity
GO:0046872--F:metal ion binding
GO:0008233--F:peptidase activity
GO:0004540--F:ribonuclease activity
GO:0003723--F:RNA binding
GO:0003964--F:RNA-directed DNA polymerase activity
GO:0004523--F:RNA-DNA hybrid ribonuclease activity
GO:0071897--P:DNA biosynthetic process
GO:0015074--P:DNA integration
GO:0006310--P:DNA recombination
GO:0090501--P:RNA phosphodiester bond hydrolysis
GO:0006278--P:RNA-dependent DNA replication
GO:0032197--P:transposition, RNA-mediated
GO:0019076--P:viral release from host cell