dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016703
Uniprot Accession Q12495; RLF2_YEAST; D6W428;
Genbank Protein ID NP_015343.1;
Genbank Nucleotide ID NM_001184115.1;
Protein Name Chromatin assembly factor 1 subunit p90
Protein Synonyms/Alias CAF-1 90 kDa subunit;RAP1 localization factor 2;
Gene Name RLF2
Gene Synonyms/Alias CAC1;YPR018W;YP9531.12;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p90 may facilitate the efficient and timely assembly of histones into telomeric chromatin.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
7-MEQHLKSIPLQDDTPhosphoGRID 2.0;curated19823750; 19795423; 22817900
14SIPLQDDTKKKGILSPhosphoGRID 2.0;curated19823750; 19795423; 22817900
77ECADEMKTTPKKANAcurated26040406
78CADEMKTTPKKANAEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19060867; 19366988; 19779198;
91AEDKLLCYKNSPIQSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900
94KLLCYKNSPIQSTKYdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 20702584; 19823750; 19779198;
133PCSKRELSSSKKEEAcurated25315811
279TKVELEGSKRKIDDEdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
331QMPLKEKTDDELQSLcurated22817900
493DPFKEPKTSSLPSKRcurated19823750; 20377248
495FKEPKTSSLPSKRSNPhosphoGRID 2.0;curated21177495; 22817900
498PKTSSLPSKRSNSDLPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900; 19823750
501SSLPSKRSNSDLQAQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 21177495; 20702584; 19684113;
503LPSKRSNSDLQAQTAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19823750; 19795423;
509NSDLQAQTASQSQSPPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated19779198; 19060867; 22817900
511DLQAQTASQSQSPEKPhosphoGRID 2.0;PhosphoPep 2.0;curated19684113; 19060867; 26040406; 22817900; 25315811
513QAQTASQSQSPEKKQPhosphoGRID 2.0;PhosphoPep 2.0;curated20702584; 19060867; 26040289; 22817900; 19823750; 25315811;
515QTASQSQSPEKKQKAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 21177495; 19779198; 19060867;
603RANANMPTPSL----curated26040289
Sequence
(Fasta)		MEQHLKSIPL QDDTKKKGIL SFFQNTTTVK SNKFLTKEKD VITLDDPKED VSGPMIETVK 60
QETMKSINKE CADEMKTTPK KANAEDKLLC YKNSPIQSTK YDRNTNKQVP NGNIIAIETK 120
SRSSSPCSKR ELSSSKKEEA KREKELKKQQ RAEEKHRKEL LRQEEKKKKE LKVEEERQRR 180
AELKKQKEEE KRRKEEARLE AKRRKEEERL KKEEEIRLKE EAKERAQSRI GNFFKKLSDS 240
NTPVVEKSDY EKFFLPFYAK DGVRVSNKWK LTKVELEGSK RKIDDELLNS KDKTSSDDLL 300
NWLQSRRLPR GHKIKRKAVD VLQQMPLKEK TDDELQSLLA QVPHKYIKFY ENVRPPFIGT 360
YSMDFTLPPN DPFSTKGTGF NYDYDSDVEW VNEEEEGEVD NLESGEEEEE EEDDEDVPSE 420
GEFDGFLDSE ENSDLDGLPC AKRKFVGPLI PTICLKSNFE NLSEENKRYL QQLKAEVIIE 480
TDGPIDPFKE PKTSSLPSKR SNSDLQAQTA SQSQSPEKKQ KAMITDPMDL LRLFDGVQDS 540
TFSLGTVTEI AQKNLPQYNK QTIKNTIKEY AIRSSGKGDL PRKWVIKDAQ NWENLRANAN 600
MPTPSL 607
Keyword

KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR022043--CAF1A
PROSITE
Pfam

PF12253--CAF1A
Gene Ontology

GO:0033186--C:CAF-1 complex
GO:0000775--C:chromosome, centromeric region
GO:0005634--C:nucleus
GO:0006335--P:DNA replication-dependent nucleosome assembly