dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016685
Uniprot Accession Q12464; RUVB2_YEAST; D6W3D5;
Genbank Protein ID NP_015089.1;
Genbank Nucleotide ID NM_001184049.1;
Protein Name RuvB-like protein 2
Protein Synonyms/Alias
Gene Name RVB2
Gene Synonyms/Alias TIH2;TIP49B;YPL235W;P1060;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing.
Phosphorylation Sites
 dbPAF PTMs: 10
PositionPeptidesSourceReferences ( PMIDs )
2------MSIQTSDPNPhosphoGRID 2.021126336
6--MSIQTSDPNETSDcurated26040289
11QTSDPNETSDLKSLScurated22817900; 19823750
78VLVAGPPSTGKTALAcurated22369663
148VEIQIDRSITGGHKQcurated25315811
212SFARSRDYDAMGADTdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
358SIIITTKSYNEQEIKcurated22817900
461VQISIAKSADPDAMDcurated25315811
469ADPDAMDTTE-----curated25521595
470DPDAMDTTE------PhosphoPep 2.0;curated19060867; 26040406; 25521595; 25315811
Sequence
(Fasta)		MSIQTSDPNE TSDLKSLSLI AAHSHITGLG LDENLQPRPT SEGMVGQLQA RRAAGVILKM 60
VQNGTIAGRA VLVAGPPSTG KTALAMGVSQ SLGKDVPFTA IAGSEIFSLE LSKTEALTQA 120
FRKSIGIKIK EETELIEGEV VEIQIDRSIT GGHKQGKLTI KTTDMETIYE LGNKMIDGLT 180
KEKVLAGDVI SIDKASGKIT KLGRSFARSR DYDAMGADTR FVQCPEGELQ KRKTVVHTVS 240
LHEIDVINSR TQGFLALFTG DTGEIRSEVR DQINTKVAEW KEEGKAEIVP GVLFIDEVHM 300
LDIECFSFIN RALEDEFAPI VMMATNRGVS KTRGTNYKSP HGLPLDLLDR SIIITTKSYN 360
EQEIKTILSI RAQEEEVELS SDALDLLTKT GVETSLRYSS NLISVAQQIA MKRKNNTVEV 420
EDVKRAYLLF LDSARSVKYV QENESQYIDD QGNVQISIAK SADPDAMDTT E 472
Keyword

KW-0010--Activator
KW-0067--ATP-binding
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0347--Helicase
KW-0378--Hydrolase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0698--rRNA processing
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR003593--AAA+_ATPase
IPR027417--P-loop_NTPase
IPR027238--RuvB-like
IPR010339--TIP49_C
PROSITE
Pfam

PF06068--TIP49
Gene Ontology

GO:0031011--C:Ino80 complex
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0097255--C:R2TP complex
GO:0000812--C:Swr1 complex
GO:0005524--F:ATP binding
GO:0043140--F:ATP-dependent 3'-5' DNA helicase activity
GO:0043141--F:ATP-dependent 5'-3' DNA helicase activity
GO:0000492--P:box C/D snoRNP assembly
GO:0006338--P:chromatin remodeling
GO:0032508--P:DNA duplex unwinding
GO:0006281--P:DNA repair
GO:0043486--P:histone exchange
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006364--P:rRNA processing
GO:0006351--P:transcription, DNA-templated