dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016682
Uniprot Accession Q12457; NSI1_YEAST; D6VS11;
Genbank Protein ID NP_010309.3;
Genbank Nucleotide ID NM_001180334.3;
Protein Name RNA polymerase I termination factor
Protein Synonyms/Alias NTS1 silencing protein 1;
Gene Name NSI1
Gene Synonyms/Alias YDR026C;PZE570;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 DNA-binding protein that recognizes sequence-specific replication termini (Ter sites) within rDNA (PubMed:14576157). Binds to rDNA terminator elements and mediates efficient RNA polymerase I transcription termination (PubMed:22805593). Required for rDNA silencing at the non-transcribed spacer 1 (NTS1). Promotes the association of SIR2 with NTS1 and contributes to maintenance of rDNA stability (PubMed:22362748).
Phosphorylation Sites
 dbPAF PTMs: 3
PositionPeptidesSourceReferences ( PMIDs )
64MSHEDEDTDMDWYLKPhosphoGRID 2.0;UniProt;curated19779198; 22817900
202ENNFNNNSSTLVRIYcurated22369663; 25315811
235IPEKDVKSDDLILAPcurated24961812
Sequence
(Fasta)		MDSVSNLKST NFQNNNDPKE SVEEAVLRYV GVDLKNHIKK TKKKLKKQKK RKHGSKMSHE 60
DEDTDMDWYL KTSGSKDLRK VDDIEPNSVA VAAVAAAYNS SMREKDKRSC HKKSSNSRSE 120
RKKHRKRKSS KERKAKIKMV LDPQLTTLDD GITTTAFLPD DLIAETAFDK YVDTEKAYLA 180
KHPSKSLEVN EDDKENNFNN NSSTLVRIYT DLEGIPNDGS YIKRTPKIPE KDVKSDDLIL 240
APEENNGDTA LLRSDIVKAS VIDGAITKSI GKKFTPSEEN ALDQFIEEYM KIRGLDRRQM 300
CERIWSTDGV IRDGFWANIS KVLPYRTRSS IYKHIRRKYH IFEQRGKWTP EEDQELARLC 360
LEKEGHWTEV GKLLGRMPED CRDRWRNYMK CGSKRGSKRW SKEEEELLTT VVNEMIEEAH 420
QYQRMKALEA ANKNDRYNQM YSRGPKGKRI SDNPTFKDMI NWTVVSERMS GTRSRIQCRY 480
KWNKLVTDEA ARSMLSIPVS ERKWLLERLK QLPKTSYSNI DWNSIATYKP GYPRTGLELR 540
LCYEQMREKI HDFKGRSTAE IIDSLLEQIN 571
Keyword

KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
Interpro

IPR009057--Homeodomain-like
IPR017877--Myb-like_dom
IPR017930--Myb_dom
IPR001005--SANT/Myb
PROSITE

PS51294--HTH_MYB
PS50090--MYB_LIKE
Pfam
Gene Ontology

GO:0005730--C:nucleolus
GO:0033553--C:rDNA heterochromatin
GO:0003677--F:DNA binding
GO:0000183--P:chromatin silencing at rDNA
GO:1900008--P:negative regulation of extrachromosomal rDNA circle accumulation involved in replicative cell aging
GO:0001185--P:termination of RNA polymerase I transcription from promoter for nuclear large rRNA transcript