dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0016670
Uniprot Accession Q12440; APC2_YEAST; D6VYC2; Q2VQX4; Q7LGV7;
Genbank Protein ID NP_013228.1;
Genbank Nucleotide ID NM_001182014.1;
Protein Name Anaphase-promoting complex subunit 2
Protein Synonyms/Alias
Gene Name APC2
Gene Synonyms/Alias RSI1;YLR127C;L3105;L3108;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.
Phosphorylation Sites
dbPAF PTMs: 1
PositionPeptidesSourceReferences ( PMIDs )
76NNATTSPYSMINTSQdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
Sequence
(Fasta)
MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH QLRPPSLRIK 60
NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL QLFSTLKEFY IFQVRYHFFL 120
HFNNINYLKD IQRWENYYEF PLRYVPIFDV NVNDWALELN SLRHYLLNRN IKFKNNLRTR 180
LDKLIMDDDF DLADNLIQWL KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM 240
ETFNKFINQY WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 300
TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA FLVLDPTGRC 360
LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF NVDMKALLSL VDTLHDSDIN 420
QDTNITKRDK NKKSPFLWNL KVKGKRELNK DLPIRHAMLY EHILNYYIAW VPEPNDMIPG 480
NIKSSYIKTN LFEVLLDLFE SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI 540
VKFTETSHSN YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 600
DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP GRKLQLCKDK 660
GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL SLEQLSESLN IAPPRLTHLL 720
DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ KTAPMEIENS NYELHNDSEI ERKYELTLQR 780
SLPFIEGMLT NLGAMKLHKI HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN 840
GSYEIVKNGH KNS 854
Keyword

KW-0002--3D-structure
KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0498--Mitosis
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR014786--APC_su2_C
IPR016158--Cullin_homology
IPR001373--Cullin_N
IPR011991--WHTH_DNA-bd_dom

PROSITE

PS50069--CULLIN_2

Pfam

PF08672--ANAPC2
PF00888--Cullin

Gene Ontology

GO:0005680--C:anaphase-promoting complex
GO:0005737--C:cytoplasm
GO:0031625--F:ubiquitin protein ligase binding
GO:0031145--P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0051301--P:cell division
GO:0010458--P:exit from mitosis
GO:0007091--P:metaphase/anaphase transition of mitotic cell cycle
GO:0008054--P:negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process