dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016656
Uniprot Accession Q12416; WHI5_YEAST; D6W2E5;
Genbank Protein ID NP_014726.1;
Genbank Nucleotide ID NM_001183502.1;
Protein Name G1-specific transcriptional repressor WHI5
Protein Synonyms/Alias
Gene Name WHI5
Gene Synonyms/Alias YOR083W;YOR3116W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Transcriptional repressor that negatively regulates G1-specific, SBF- and MBF-dependent transcription. Binds via SBF to promoters of G1-specific genes to repress transcription in early G1. During G1, phosphorylated by cyclin-CDK CLN3-CDC28 and progressively hyperphosphorylated by CDK associated with other G1-cyclins, which leads to dissociation of WHI5 from SBF, activating SBF-dependent transcription in late G1. Elimination of CDK activity at the end of mitosis by the mitotic exit network (MEN) allows WHI5 to reassociate with SBF to again repress G1/S transcription.
Phosphorylation Sites
 dbPAF PTMs: 34 (View all)
PositionPeptidesSourceReferences ( PMIDs )
5---MSLRTPKRSRTSPhosphoGRID 2.0;curated19520826; 19172996; 19823750
47GPTTLLSTPVRLKNGdbPTM 3.0;PhosphoGRID 2.0;UniProt15210110; 16381945; 23193290; 19172996
57RLKNGFGTPSPPSPPPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated; 21177495; 21126336; 19684113; 19172996; 19779198; 19060867
59KNGFGTPSPPSPPGIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;UniProt;curated15210110; 17563356; 16381945; 23193290; 21177495; 19684113;
62FGTPSPPSPPGITKSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15210110; 15665377; 17563356; 18407956; 16381945; 23193290;
67PPSPPGITKSITKSRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 19060867; 19366988;
71PGITKSITKSRRRPScurated22817900
73ITKSITKSRRRPSTTcurated24961812
78TKSRRRPSTTSLQGIPhosphoGRID 2.0;PhosphoPep 2.0;curated17287358; 19060867; 26040406; 22817900; 24961812; 25315811
79KSRRRPSTTSLQGIFPhosphoGRID 2.0;PhosphoPep 2.0;curated19684113; 17287358; 19060867; 22817900; 24961812; 25882841;
80SRRRPSTTSLQGIFMPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 22817900; 24961812
81RRRPSTTSLQGIFMSPhosphoPep 2.0;curated19060867; 22369663; 22817900; 24961812; 25882841; 25315811
88SLQGIFMSPVNKRRVPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated19684113; 19172996; 19779198; 19060867; 26040289; 22817900;
104ITAHGRVYDHNDDGHcurated22817900
113HNDDGHESESEDDENdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 19684113;
115DDGHESESEDDENEEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 19684113;
142SMPLLPPTTPKSRRSPhosphoPep 2.019060867
143MPLLPPTTPKSRRSEPhosphoGRID 2.0;PhosphoPep 2.0;curated19172996; 19060867; 24961812
146LPPTTPKSRRSEVFLPhosphoPep 2.0;curated19060867; 22817900
149TTPKSRRSEVFLSPSPhosphoGRID 2.0;curated19172996; 22817900; 25315811
Sequence
(Fasta)		MSLRTPKRSR TSDEQEQEQE QEQVQNPDTH VNNEHQQRPG PTTLLSTPVR LKNGFGTPSP 60
PSPPGITKSI TKSRRRPSTT SLQGIFMSPV NKRRVGITAH GRVYDHNDDG HESESEDDEN 120
EEENENQKKY DGHVSMPLLP PTTPKSRRSE VFLSPSPRLR SPPTAARRST GERPIREISH 180
TLRTRLNYAL VKLQNGWTDK TLPELETELA PAVQTPPRRY HNRFPDSADA GTSAHTAFLQ 240
ALGGHPPREE ATAVETLMLL SSPTKKQQHR PVPATSAGEP TDETEPESDT EVETS 296
Keyword

KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR013734--TF_Nrm1/Whi5
PROSITE
Pfam

PF08528--Whi5
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0033309--C:SBF transcription complex
GO:0001135--F:transcription factor activity, RNA polymerase II transcription factor recruiting
GO:0001191--F:transcriptional repressor activity, RNA polymerase II transcription factor binding
GO:0071930--P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0006351--P:transcription, DNA-templated