dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016643
Uniprot Accession Q12389; DBP10_YEAST; D6VRW1;
Genbank Protein ID NP_010253.2;
Genbank Nucleotide ID NM_001180090.2;
Protein Name ATP-dependent RNA helicase DBP10
Protein Synonyms/Alias DEAD box protein 10;
Gene Name DBP10
Gene Synonyms/Alias YDL031W;D2770;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.
Phosphorylation Sites
 dbPAF PTMs: 13
PositionPeptidesSourceReferences ( PMIDs )
101AFPMLEMSDDENNASdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 20702584; 19823750;
108SDDENNASGKTQTGDPHOSIDA;curated21081558; 22817900; 19823750
111ENNASGKTQTGDDEDcurated25315811
113NASGKTQTGDDEDDVcurated24961812
392QLQKLQNSNNEADSDPhosphoGRID 2.0;PhosphoPep 2.0;curated20702584; 19060867; 22817900
398NSNNEADSDSDDENDdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17563356; 18407956; 16381945; 23193290; 20702584;
400NNEADSDSDDENDRQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17563356; 18407956; 16381945; 23193290; 20702584;
563LLTPMYDSLVDVMKKcurated20377248
626DLQLAKKTAMKAEKLPHOSIDA;curated21081558; 22817900; 19795423
819NDAAQAAYDLNSDDKcurated26040289
823QAAYDLNSDDKVQVHcurated26040289
868SGQKIAASFRSGRFDPhosphoGRID 2.021126336
949KVEKALQSGISVKGYPhosphoGRID 2.021126336
Sequence
(Fasta)		MAGVQKRKRD LEDQDDNGSE EDDIAFDIAN EIALNDSESD ANDSDSEVEA DYGPNDVQDV 60
IEYSSDEEEG VNNKKKAENK DIKKKKNSKK EIAAFPMLEM SDDENNASGK TQTGDDEDDV 120
NEYFSTNNLE KTKHKKGSFP SFGLSKIVLN NIKRKGFRQP TPIQRKTIPL ILQSRDIVGM 180
ARTGSGKTAA FILPMVEKLK SHSGKIGARA VILSPSRELA MQTFNVFKDF ARGTELRSVL 240
LTGGDSLEEQ FGMMMTNPDV IIATPGRFLH LKVEMNLDLK SVEYVVFDEA DRLFEMGFQE 300
QLNELLASLP TTRQTLLFSA TLPNSLVDFV KAGLVNPVLV RLDAETKVSE NLEMLFLSSK 360
NADREANLLY ILQEIIKIPL ATSEQLQKLQ NSNNEADSDS DDENDRQKKR RNFKKEKFRK 420
QKMPAANELP SEKATILFVP TRHHVEYISQ LLRDCGYLIS YIYGTLDQHA RKRQLYNFRA 480
GLTSILVVTD VAARGVDIPM LANVINYTLP GSSKIFVHRV GRTARAGNKG WAYSIVAENE 540
LPYLLDLELF LGKKILLTPM YDSLVDVMKK RWIDEGKPEY QFQPPKLSYT KRLVLGSCPR 600
LDVEGLGDLY KNLMSSNFDL QLAKKTAMKA EKLYYRTRTS ASPESLKRSK EIISSGWDAQ 660
NAFFGKNEEK EKLDFLAKLQ NRRNKETVFE FTRNPDDEMA VFMKRRRKQL APIQRKATER 720
RELLEKERMA GLSHSIEDEI LKGDDGETGY TVSEDALKEF EDADQLLEAQ ENENKKKKKP 780
KSFKDPTFFL SHYAPAGDIQ DKQLQITNGF ANDAAQAAYD LNSDDKVQVH KQTATVKWDK 840
KRKKYVNTQG IDNKKYIIGE SGQKIAASFR SGRFDDWSKA RNLKPLKVGS RETSIPSNLL 900
EDPSQGPAAN GRTVRGKFKH KQMKAPKMPD KHRDNYYSQK KKVEKALQSG ISVKGYNNAP 960
GLRSELKSTE QIRKDRIIAE KKRAKNARPS KKRKF 996
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0347--Helicase
KW-0378--Hydrolase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0690--Ribosome biogenesis
KW-0694--RNA-binding
KW-0698--rRNA processing
Interpro

IPR012541--DBP10CT
IPR011545--DEAD/DEAH_box_helicase_dom
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR027417--P-loop_NTPase
IPR000629--RNA-helicase_DEAD-box_CS
IPR014014--RNA_helicase_DEAD_Q_motif
PROSITE

PS00039--DEAD_ATP_HELICASE
PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER
PS51195--Q_MOTIF
Pfam

PF08147--DBP10CT
PF00270--DEAD
PF00271--Helicase_C
Gene Ontology

GO:0005730--C:nucleolus
GO:0030687--C:preribosome, large subunit precursor
GO:0005524--F:ATP binding
GO:0004004--F:ATP-dependent RNA helicase activity
GO:0042802--F:identical protein binding
GO:0003723--F:RNA binding
GO:1902626--P:assembly of large subunit precursor of preribosome
GO:0000466--P:maturation of 5
8S rRNA fr--m tricistronic rRNA transcript (SSU-rRNA, 5
8S rRNA, L--U-rRNA)
GO:0000463--P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5
8S rRNA, L--U-rRNA)
GO:0010501--P:RNA secondary structure unwinding