dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016641
Uniprot Accession Q12386; ARP8_YEAST; D6W2J7;
Genbank Protein ID NP_014784.1;
Genbank Nucleotide ID NM_001183560.1;
Protein Name Actin-like protein ARP8
Protein Synonyms/Alias
Gene Name ARP8
Gene Synonyms/Alias YOR141C;YOR3348C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex. Exhibits low basal ATPase activity, and unable to polymerize. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.
Phosphorylation Sites
 dbPAF PTMs: 11
PositionPeptidesSourceReferences ( PMIDs )
65VGSETPRSVTGLSVDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 20702584; 19779198;
67SETPRSVTGLSVDPRcurated22369663
70PRSVTGLSVDPRDVAdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22369663;
130RAPPAVQTSKRYKKYPhosphoGRID 2.0;curated19779198; 24603354; 22817900
230FLRNNNTSQISSTNTdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
233NNNTSQISSTNTPDVPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 22369663; 22817900; 24961812; 25315811;
234NNTSQISSTNTPDVIcurated24961812
235NTSQISSTNTPDVIDdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 20377248
237SQISSTNTPDVIDLKPhosphoPep 2.0;curated19060867; 22369663; 22817900; 24961812; 25315811; 20377248;
320VCLQREQSEEFNNIKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 25315811
381IEWIFDDSKLYYGSDcurated22817900
Sequence
(Fasta)		MSQEEAESSI IYEEPIDIPL EDDDDEDELE EENSVPLSSQ ADQENAENES DDSVDNVVGS 60
ETPRSVTGLS VDPRDVADEE DEDEEGEDED EDEDDNDVDN EDENDNDNAN ENENELGSSR 120
DKRAPPAVQT SKRYKKYPKL DPAKAPPGKK VPLHLLEKRR LGRIKAAEEF AKTLKKIGIE 180
KVETTTLPAT GLFQPLMLIN QKNYSSDYLK KDDQIFALRD RKFLRNNNTS QISSTNTPDV 240
IDLKSLPHSE ASAAPLNDEI DLNDPTATIV IHPGSNSIKI GFPKDDHPVV VPNCVAVPKK 300
WLDLENSEHV ENVCLQREQS EEFNNIKSEM EKNFRERMRY YKRKVPGNAH EQVVSFNENS 360
KPEIISEKND PSPIEWIFDD SKLYYGSDAL RCVDEKFVIR KPFRGGSFNV KSPYYKSLAE 420
LISDVTKLLE HALNSETLNV KPTKFNQYKV VLVIPDIFKK SHVETFIRVL LTELQFQAVA 480
IIQESLATCY GAGISTSTCV VNIGAAETRI ACVDEGTVLE HSAITLDYGG DDITRLFALF 540
LLQSDFPLQD WKIDSKHGWL LAERLKKNFT TFQDADVAVQ LYNFMNRSPN QPTEKYEFKL 600
FDEVMLAPLA LFFPQIFKLI RTSSHKNSSL EFQLPESRDL FTNELNDWNS LSQFESKEGN 660
LYCDLNDDLK ILNRILDAHN IIDQLQDKPE NYGNTLKENF APLEKAIVQS IANASITADV 720
TRMNSFYSNI LIVGGSSKIP ALDFILTDRI NIWRPSLLSS ASFPQFYKKL TKEIKDLEGH 780
YVNAPDKTED ENKQILQAQI KEKIVEELEE QHQNIEHQNG NEHIFPVSII PPPRDMNPAL 840
IIWKGASVLA QIKLVEELFI TNSDWDVHGS RILQYKCIFT Y 882
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR004000--Actin
IPR027668--Arp8/plant_Arp9
PROSITE
Pfam

PF00022--Actin
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005856--C:cytoskeleton
GO:0031011--C:Ino80 complex
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0003729--F:mRNA binding
GO:0006974--P:cellular response to DNA damage stimulus
GO:0006338--P:chromatin remodeling
GO:0032508--P:DNA duplex unwinding
GO:0006302--P:double-strand break repair
GO:0006312--P:mitotic recombination
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated