dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016577
Uniprot Accession Q12256; TPO4_YEAST; D6W2X3;
Genbank Protein ID NP_014916.1;
Genbank Nucleotide ID NM_001183692.1;
Protein Name Polyamine transporter 4
Protein Synonyms/Alias
Gene Name TPO4
Gene Synonyms/Alias YOR273C;O5440;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine.
Phosphorylation Sites
 dbPAF PTMs: 33 (View all)
PositionPeptidesSourceReferences ( PMIDs )
3-----MPSSLTKTESPhosphoPep 2.0;curated19060867; 26040406; 26040289
4----MPSSLTKTESNPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19795423; 19060867; 26040406; 24961812
6--MPSSLTKTESNSDPHOSIDA;PhosphoPep 2.0;curated21081558; 19060867; 22369663; 24603354; 19795423; 23825934
8MPSSLTKTESNSDPRPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19795423; 19060867; 26040406; 26040289; 22369663;
10SSLTKTESNSDPRTNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19060867; 19366988; 26040406;
12LTKTESNSDPRTNIQPHOSIDA;PhosphoPep 2.0;curated21081558; 19060867; 26040406; 19795423; 19823750; 24961812
37TNSGNLDSTSSSTGScurated22369663
40GNLDSTSSSTGSITEcurated20377248
41NLDSTSSSTGSITEDcurated20377248
44STSSSTGSITEDEKRcurated26040289; 22369663; 24961812; 25315811
52ITEDEKRSEPNADSNPhosphoGRID 2.0;PHOSIDA;curated19779198; 21081558; 26040289; 22369663; 19795423
58RSEPNADSNNMTGGEcurated26040289
300AQKEFLKTTVTITILcurated23825934
305LKTTVTITILRPLKMcurated23825934
579VDGELNLTRMTTLRTcurated26040289
583LNLTRMTTLRTMETDPhosphoGRID 2.019779198
586TRMTTLRTMETDPSTPhosphoGRID 2.0;curated19779198; 22369663; 25315811
589TTLRTMETDPSTREKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated11875433; 18407956; 16381945; 23193290; 19779198; 19366988;
592RTMETDPSTREKPGEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated11875433; 16381945; 23193290; 19779198; 19366988; 26040406;
593TMETDPSTREKPGERPhosphoGRID 2.0;curated19779198; 22369663; 22817900
Sequence
(Fasta)		MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK RSEPNADSNN 60
MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF LCLVVTMGSS LYVSSVPELV 120
ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM 180
RIILPLRFLS GVFASPALSV GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT 240
EAKGWRWSEW IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT 300
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM SMGISGLPFI 360
GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK RTTPYRGARD AETGELLPVV 420
PEKFLIACKF GSVALPIGLF WQAWTARSDV HWMAPVAAGV PFGFGLILIF FSVLMYFSTC 480
YPPLTVASCL AANNLLRYVM SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK 540
WGSKLRHKSQ FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER 600
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM ATDASARMV 660
Keyword

KW-0050--Antiport
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0813--Transport
Interpro

IPR011701--MFS
IPR020846--MFS_dom
IPR005829--Sugar_transporter_CS
PROSITE

PS00216--SUGAR_TRANSPORT_1
Pfam

PF07690--MFS_1
Gene Ontology

GO:0000329--C:fungal-type vacuole membrane
GO:0016021--C:integral component of membrane
GO:0005886--C:plasma membrane
GO:0015297--F:antiporter activity
GO:0015606--F:spermidine transmembrane transporter activity
GO:0000297--F:spermine transmembrane transporter activity
GO:1903711--P:spermidine transmembrane transport
GO:0015848--P:spermidine transport
GO:1903710--P:spermine transmembrane transport
GO:0000296--P:spermine transport