| Tag |
Content |
| dbPAF ID |
dbPAF-0016571 |
| Uniprot Accession |
Q12248; DAD1_YEAST; D6VS02;
|
| Genbank Protein ID |
NP_010299.3;
|
| Genbank Nucleotide ID |
NM_001180324.3;
|
| Protein Name |
DASH complex subunit DAD1 |
| Protein Synonyms/Alias |
DUO1 and DAM1-interacting protein 1;Outer kinetochore protein DAD1; |
| Gene Name |
DAD1 |
| Gene Synonyms/Alias |
YDR016C; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.Functional Description
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. |
Phosphorylation Sites
|
dbPAF PTMs: 4 |
Sequence
(Fasta) | MMASTSNDEE KLISTTDKYF IEQRNIVLQE INETMNSILN GLNGLNISLE SSIAVGREFQ 60
SVSDLWKTLY DGLESLSDEA PIDEQPTLSQ SKTK
95Fasta Sequence
>Q12248|DAD1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MMASTSNDEEKLISTTDKYFIEQRNIVLQEINETMNSILNGLNGLNISLESSIAVGREFQSVSDLWKTLYDGLESLSDEAPIDEQPTLSQSKTK
|
| Keyword |
KW-0131--Cell cycle
KW-0132--Cell division
KW-0137--Centromere
KW-0158--Chromosome
KW-0159--Chromosome partition
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0995--Kinetochore
KW-0493--Microtubule
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
|
| Interpro |
IPR013958--DASH_Dad1
|
| PROSITE |
|
| Pfam |
PF08649--DASH_Dad1
|
| Gene Ontology |
GO:0005737--C:cytoplasm
GO:0042729--C:DASH complex
GO:0005874--C:microtubule
GO:0072686--C:mitotic spindle
GO:0008608--P:attachment of spindle microtubules to kinetochore
GO:0051301--P:cell division
GO:0007067--P:mitotic nuclear division
GO:0030472--P:mitotic spindle organization in nucleus
GO:0051987--P:positive regulation of attachment of spindle microtubules to kinetochore
GO:0031116--P:positive regulation of microtubule polymerization
|
