dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016570
Uniprot Accession Q12246; LCB4_YEAST; D6W2M7;
Genbank Protein ID NP_014814.1;
Genbank Nucleotide ID NM_001183590.1;
Protein Name Sphingoid long chain base kinase 4
Protein Synonyms/Alias
Gene Name LCB4
Gene Synonyms/Alias YOR171C;O3615;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Involved in the biosynthesis of sphingolipids and ceramides. Required with LCB3 for an effective incorporation of DHS into ceramides through a phosphorylation-dephosphorylation cycle. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance.
Phosphorylation Sites
 dbPAF PTMs: 22 (View all)
PositionPeptidesSourceReferences ( PMIDs )
108PFQTENLSSSSENDDPHOSIDA;curated21081558; 22817900; 19795423; 24961812; 20377248
109FQTENLSSSSENDDVPHOSIDA;curated21081558; 19795423; 24961812; 25315811; 20377248
110QTENLSSSSENDDVEcurated22817900; 20377248
111TENLSSSSENDDVENPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 24961812; 25315811; 20377248
120NDDVENHSLSNDKAPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
122DVENHSLSNDKAPVScurated22817900; 25315811
133APVSESQSFPKKDKWcurated25315811
150KTNTVKVSPDDSQDNPhosphoGRID 2.021126336
154VKVSPDDSQDNSPSLPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated20489023; 21126336; 20702584; 19823750; 19779198; 19795423;
158PDDSQDNSPSLGIKDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 20489023; 20702584; 19823750;
160DSQDNSPSLGIKDNQdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988; 22369663; 24961812
198IDHVSRKSRANTGEEPhosphoGRID 2.0;curated19823750; 19795423; 22817900; 24961812
202SRKSRANTGEENISSPhosphoGRID 2.0;curated19823750; 19795423; 22817900; 24961812
340CHWTNNPSYAALCLVcurated22817900
451LTFEPNPSPNSSPDLdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated15598647; 17330950; 16381945; 23193290; 20702584; 19684113;
454EPNPSPNSSPDLLSKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 20702584; 19779198;
455PNPSPNSSPDLLSKNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated15598647; 17330950; 18407956; 16381945; 23193290; 20702584;
460NSSPDLLSKNNINNSPhosphoGRID 2.0;PHOSIDA;UniProt;curated19684113; 19779198; 21081558; 22369663; 22817900; 19795423
467SKNNINNSTKDELSPPHOSIDA;curated21081558; 26040289; 19795423; 24961812; 25315811
468KNNINNSTKDELSPNdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 22369663; 22817900; 25315811;
Sequence
(Fasta)		MVVQKKLRAI LTDEGVLIKS QSHHMFNKHG QLRSGDSLSL LSCLSCLDDG TLSSDGGSFD 60
EDDSLELLPL NTTIPFNRIL NAKYVNVGQK GFNNGKISSN PFQTENLSSS SENDDVENHS 120
LSNDKAPVSE SQSFPKKDKW DTKTNTVKVS PDDSQDNSPS LGIKDNQQLI ELTFAVPKGH 180
DVIPQKLTLL IDHVSRKSRA NTGEENISSG TVEEILEKSY ENSKRNRSIL VIINPHGGKG 240
TAKNLFLTKA RPILVESGCK IEIAYTKYAR HAIDIAKDLD ISKYDTIACA SGDGIPYEVI 300
NGLYRRPDRV DAFNKLAVTQ LPCGSGNAMS ISCHWTNNPS YAALCLVKSI ETRIDLMCCS 360
QPSYMNEWPR LSFLSQTYGV IAESDINTEF IRWMGPVRFN LGVAFNIIQG KKYPCEVFVK 420
YAAKSKKELK VHFLENKDKN KGCLTFEPNP SPNSSPDLLS KNNINNSTKD ELSPNFLNED 480
NFKLKYPMTE PVPRDWEKMD SELTDNLTIF YTGKMPYIAK DTKFFPAALP ADGTIDLVIT 540
DARIPVTRMT PILLSLDKGS HVLEPEVIHS KILAYKIIPK VESGLFSVDG EKFPLEPLQV 600
EIMPMLCKTL LRNGRYIDTE FESM 625
Keyword

KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0256--Endoplasmic reticulum
KW-0967--Endosome
KW-0333--Golgi apparatus
KW-0418--Kinase
KW-0443--Lipid metabolism
KW-0449--Lipoprotein
KW-0472--Membrane
KW-0547--Nucleotide-binding
KW-0564--Palmitate
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0746--Sphingolipid metabolism
KW-0808--Transferase
KW-0832--Ubl conjugation
Interpro

IPR001206--Diacylglycerol_kinase_cat_dom
IPR016064--NAD/diacylglycerol_kinase
PROSITE

PS50146--DAGK
Pfam

PF00781--DAGK_cat
Gene Ontology

GO:0032541--C:cortical endoplasmic reticulum
GO:0005783--C:endoplasmic reticulum
GO:0005789--C:endoplasmic reticulum membrane
GO:0005794--C:Golgi apparatus
GO:0000139--C:Golgi membrane
GO:0031902--C:late endosome membrane
GO:0005886--C:plasma membrane
GO:0005524--F:ATP binding
GO:0017050--F:D-erythro-sphingosine kinase activity
GO:0008481--F:sphinganine kinase activity
GO:0019722--P:calcium-mediated signaling
GO:0006665--P:sphingolipid metabolic process