dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016566
Uniprot Accession Q12236; PKH2_YEAST; D6W1W8;
Genbank Protein ID NP_014541.1;
Genbank Nucleotide ID NM_001183354.1;
Protein Name Serine/threonine-protein kinase PKH2
Protein Synonyms/Alias PKB-activating kinase homolog 2;
Gene Name PKH2
Gene Synonyms/Alias YOL100W;HRC1081;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Serine/threonine-protein kinase which is part sphingolipid-mediated signaling pathway that is required for the internalization step of endocytosis by regulating eisosome assembly and organization, and modulating the organization of the plasma membrane. Phosphorylates and activates PKC1. Activates YPK1 and YPK2, 2 components of signaling cascade required for maintenance of cell wall integrity. Required for stress-induced P-body assembly and regulates global mRNA decay at the deadenylation step.
Phosphorylation Sites
 dbPAF PTMs: 46 (View all)
PositionPeptidesSourceReferences ( PMIDs )
8MYFDKDNSMSPRPLLPhosphoGRID 2.0;curated20489023; 19779198; 22817900; 24961812; 25315811; 20377248
10FDKDNSMSPRPLLPSPhosphoGRID 2.0;curated20489023; 19779198; 22817900; 24961812; 20377248
49AKATPQRSTSNRNVGPhosphoGRID 2.0;PhosphoPep 2.0;curated17287358; 19060867; 22817900
50KATPQRSTSNRNVGDPhosphoGRID 2.017287358
51ATPQRSTSNRNVGDLPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 17287358; 19060867; 22369663; 22817900; 20377248
74PMIQKALTNTDNFIEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
76IQKALTNTDNFIEMYcurated25521595
95RKNLDDDTIKEVMINcurated24961812
108INDENGKTVASTNDGcurated25315811
111ENGKTVASTNDGRYDcurated24961812
131NDINDQKTLDNIAGScurated24961812
138TLDNIAGSPHMEKNRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 20489023; 20702584; 19823750;
239QKLNNSPSVVRLFSTcurated25315811
346EYDLSTRSKSFVGTAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19779198; 19060867;
348DLSTRSKSFVGTAEYPhosphoPep 2.019060867
352RSKSFVGTAEYVSPEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
560KNQPGKRTPSGAASAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19060867; 22817900
562QPGKRTPSGAASAALPhosphoPep 2.019060867
576LAASAALTKKTMQSYdbPTM 3.0;curated17563356; 16381945; 23193290; 22817900
588QSYPTSSSKSSRSSScurated22817900
Sequence
(Fasta)		MYFDKDNSMS PRPLLPSDEQ KLNINLLTKK EKFSHLDPHY DAKATPQRST SNRNVGDLLL 60
EKRTAKPMIQ KALTNTDNFI EMYHNQQRKN LDDDTIKEVM INDENGKTVA STNDGRYDND 120
YDNNDINDQK TLDNIAGSPH MEKNRNKVKI EHDSSSQKPI AKESSKAQKN IIKKGIKDFK 180
FGSVIGDGAY STVMLATSID TKKRYAAKVL NKEYLIRQKK VKYVSIEKTA LQKLNNSPSV 240
VRLFSTFQDE SSLYFLLEYA PNGDFLSLMK KYGSLDETCA RYYAAQIIDA IDYLHSNGII 300
HRDIKPENIL LDGEMKIKLT DFGTAKLLNP TNNSVSKPEY DLSTRSKSFV GTAEYVSPEL 360
LNDSFTDYRC DIWAFGCILF QMIAGKPPFK ATNEYLTFQK VMKVQYAFTP GFPLIIRDLV 420
KKILVKNLDR RLTISQIKEH HFFKDLNFKD GSVWSKTPPE IKPYKINAKS MQAMPSGSDR 480
KLVKKSVNTL GKSHLVTQRS ASSPSVEETT HSTLYNNNTH ASTESEISIK KRPTDERTAQ 540
ILENARKGIN NRKNQPGKRT PSGAASAALA ASAALTKKTM QSYPTSSSKS SRSSSPATTS 600
RPGTYKRTSS TESKPFAKSP PLSASVLSSK VPMPPYTPPM SPPMTPYDTY QMTPPYTTKQ 660
QDYSDTAIAA PKPCISKQNV KNSTDSPLMN KQDIQWSFYL KNINEHVLRT EKLDFVTTNY 720
DILEKKMLKL NGSLLDPQLF GKPRHTFLSQ VARSGGEVTG FRNDPTMTAY SKTEDTYYSK 780
NIIDLQLLED DYRIEGGDLS ELLTNRSGEG YKCNQNSSPM KDDDKSESNN KGSSVFSGKI 840
KKLFHPTSAA ETLSSSDEKT KYYKRTIVMT SFGRFLVFAK RRQPNPVTNL KYELEYDINL 900
RQQGTKIKEL IIPLEMGTNH IVVIQTPYKS FLLSTDKKTT SKLFTVLKKI LNSNTNKIEK 960
ELLQRNQKVI ERRTSSSGRA IPKDLPTSKS PSPKPRTHSQ SPSISKHNSF SESINSAKSN 1020
RSSRIFETFI NAKEQNSKKH AAPVPLTSKL VNGLPKRQVT VGLGLNTGTN FKNSSAKSKR 1080
S 1082
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0254--Endocytosis
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
Gene Ontology

GO:0005938--C:cell cortex
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004672--F:protein kinase activity
GO:0004674--F:protein serine/threonine kinase activity
GO:0033962--P:cytoplasmic mRNA processing body assembly
GO:0006897--P:endocytosis
GO:0032511--P:late endosome to vacuole transport via multivesicular body sorting pathway
GO:0000196--P:MAPK cascade involved in cell wall organization or biogenesis
GO:0006468--P:protein phosphorylation
GO:0060211--P:regulation of nuclear-transcribed mRNA poly(A) tail shortening