dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016562
Uniprot Accession Q12230; LSP1_YEAST; D6W408;
Genbank Protein ID NP_015321.1;
Genbank Nucleotide ID NM_001183818.1;
Protein Name Sphingolipid long chain base-responsive protein LSP1
Protein Synonyms/Alias
Gene Name LSP1
Gene Synonyms/Alias YPL004C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Together with PIL1, main component of eisosomes, structures at the cell periphery underneath the plasma membrane that mark the site of endocytosis. Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress.
Phosphorylation Sites
 dbPAF PTMs: 18
PositionPeptidesSourceReferences ( PMIDs )
4----MHRTYSLRNQRcurated19823750
6--MHRTYSLRNQRAPcurated19823750; 24961812
14LRNQRAPTAAELQAPPhosphoGRID 2.0;curated21177495; 21126336; 19779198; 26040406; 26040289; 22817900;
26QAPPPPPSSTKSKFFdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 19779198; 19060867;
27APPPPPSSTKSKFFGdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 19060867; 19366988;
28PPPPPSSTKSKFFGKPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900; 25315811; 19547744
30PPPSSTKSKFFGKAScurated22817900; 19547744
37SKFFGKASIASSFRKcurated22817900; 24961812; 25315811; 19547744
40FGKASIASSFRKNAAcurated24961812
41GKASIASSFRKNAAGPhosphoGRID 2.0;curated19823750; 19795423; 22817900; 25315811
59PELARKLSQLVKTEKPhosphoGRID 2.0;curated20702584; 19823750; 19795423; 22817900; 24961812; 20377248
98ADNDDDVSDVTDKLGcurated22817900; 24961812; 26040289
138SIRNIEASVQPSRDRcurated25315811
142IEASVQPSRDRKEKIPhosphoPep 2.0;curated19060867; 25315811
230LLELLDDSPVTPGEAdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 24603354; 26447709
233LLDDSPVTPGEARPAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17330950; 17563356; 18407956; 16381945; 23193290;
273AAVKPTLSFHQTVDDdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
336VSQNGHTSGSENI--dbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
Sequence
(Fasta)		MHRTYSLRNQ RAPTAAELQA PPPPPSSTKS KFFGKASIAS SFRKNAAGNF GPELARKLSQ 60
LVKTEKGVLR AMEVVASERR EAAKQLSLWG ADNDDDVSDV TDKLGVLIYE LGELQDQFID 120
KYDQYRVTLK SIRNIEASVQ PSRDRKEKIT DEIAHLKYKD PQSTKIPVLE QELVRAEAES 180
LVAEAQLSNI TREKLKAAYS YMFDSLRELS EKFALIAGYG KALLELLDDS PVTPGEARPA 240
YDGYEASRQI IMDAESALES WTLDMAAVKP TLSFHQTVDD VYEDEDGEEE EEPEIQNGDI 300
PGQVVEEEEV EWTTEVPVDD EAHEADHHVS QNGHTSGSEN I 342
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0325--Glycoprotein
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR028245--PIL1/LSP1
PROSITE
Pfam

PF13805--Pil1
Gene Ontology

GO:0005938--C:cell cortex
GO:0032126--C:eisosome
GO:0008289--F:lipid binding
GO:0006897--P:endocytosis
GO:0006469--P:negative regulation of protein kinase activity
GO:0009408--P:response to heat