dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016558
Uniprot Accession Q12221; PUF2_YEAST; D6W451; Q02573; Q04142; Q7LH42; Q7LH98;
Genbank Protein ID NP_015367.1;
Genbank Nucleotide ID NM_001184139.1;
Protein Name mRNA-binding protein PUF2
Protein Synonyms/Alias Pumilio homology domain family member 2;
Gene Name PUF2
Gene Synonyms/Alias YPR042C;YP3085.06c;YP9499.01;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 RNA-binding protein involved in post-transcriptional regulation. Negatively regulates expression of COX17 by binding to the 3'-UTR of COX17 mRNA. Promotes decay of COX17 mRNA by enhancing its rate of deadenylation and subsequent turnover. Predominantly binds to mRNAs encoding membrane-associated proteins with roles in transmembrane transport and vesicular trafficking.
Phosphorylation Sites
 dbPAF PTMs: 20
PositionPeptidesSourceReferences ( PMIDs )
72RTRAGRFSDTLTNLLPhosphoGRID 2.0;UniProt;curated19779198; 22817900
103VVVPPTSSTPDSLNSPhosphoGRID 2.019779198
104VVPPTSSTPDSLNSTcurated22369663
107PTSSTPDSLNSTTYAcurated22369663
118TTYAPRVSSDSFTVAcurated25315811
121APRVSSDSFTVATPLPhosphoGRID 2.0;curated19779198; 25315811
123RVSSDSFTVATPLSLPhosphoGRID 2.019779198
198EPLTRFKSTDNYTLPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17563356; 16381945; 23193290; 17330950; 21177495;
199PLTRFKSTDNYTLPQdbPTM 3.0;PhosphoPep 2.0;SysPTM 2.018407956; 16381945; 23193290; 19060867; 19366988
236SNTDHLNTPIVNRQRPhosphoPep 2.019060867
278VPPRVPKSTSISPVIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.018407956; 16381945; 23193290; 15665377; 19060867; 19366988
280PRVPKSTSISPVILDdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
871LLEEVGLSSAGISPKcurated22817900
872LEEVGLSSAGISPKQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19684113; 19060867; 19366988;
876GLSSAGISPKQSSKNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 19684113; 19779198;
894QHPQGFHSPGRARGVPhosphoPep 2.0;curated19060867; 22369663
902PGRARGVSVSSVRSSPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 21126336; 19684113; 19779198; 19060867; 26040406;
904RARGVSVSSVRSSNSPhosphoGRID 2.0;curated19684113; 26040289; 22817900
905ARGVSVSSVRSSNSRPhosphoGRID 2.0;curated21177495; 19684113; 19779198; 26040289; 22817900
908VSVSSVRSSNSRHNSPhosphoGRID 2.0;curated21177495; 19684113; 22817900
Sequence
(Fasta)		MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR GRSSSTSPQK 60
IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP TSSTPDSLNS TTYAPRVSSD 120
SFTVATPLSL QSTTTRTRTR NNTVSSQITA SSSLTTDVGN ATSANIWSAN AESNTSSSPL 180
FDYPLATSYF EPLTRFKSTD NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN 240
RQRSQSQSTT NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN 300
QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ YGKVLSARTL 360
RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA RVLPMYEQPL NVNGFNNTPK 420
QPLLQEQLNH GVLNYQLQQS LQQPELQQQP TSFNQPNLTY CNPTQNLSHL QLSSNENEPY 480
PFPLPPPSLS DSKKDILHTI SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK 540
VPKRKDTFDA PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN 600
SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV SDYCTPLFND 660
QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR AVRACLEADS IITQCQLLTI 720
TSLIIVLSPY LATDTNGTLL ITWLLDTCTL PNKNLILCDK LVNKNLVKLC CHKLGSLTVL 780
KILNLRGGEE EALSKNKIIH AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV 840
RDEAITKIRQ LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG 900
VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS GNQNQNQNGN 960
SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN NSGYSSQMNP LSRSVSHNNN 1020
NNTNNYNNND NDNNNNNNNN NNNNNNNNNN NNNSNNSNNN NNNDTSLYRY RSYGY 1076
Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0694--RNA-binding
Interpro

IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR012677--Nucleotide-bd_a/b_plait
IPR001313--Pumilio_RNA-bd_rpt
IPR000504--RRM_dom
PROSITE

PS50302--PUM
PS50303--PUM_HD
PS50102--RRM
Pfam

PF00806--PUF
PF00076--RRM_1
Gene Ontology

GO:0005737--C:cytoplasm
GO:0003729--F:mRNA binding
GO:0000166--F:nucleotide binding
GO:0000288--P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay