dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016536
Uniprot Accession Q12186; BBP_YEAST; D6VYB4;
Genbank Protein ID NP_013217.1;
Genbank Nucleotide ID NM_001182003.1;
Protein Name Branchpoint-bridging protein
Protein Synonyms/Alias Mud synthetic-lethal 5 protein;Splicing factor 1;Zinc finger protein BBP;
Gene Name MSL5
Gene Synonyms/Alias BBP;SF1;YLR116W;L2949;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.
Phosphorylation Sites
 dbPAF PTMs: 9
PositionPeptidesSourceReferences ( PMIDs )
93PPSRKNRSPSPPPVYdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 21126336;
95SRKNRSPSPPPVYDAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 20702584;
100SPSPPPVYDAQGKRTPhosphoGRID 2.0;UniProt;curated21177495; 19779198; 22817900; 19823750
244IVIKAVTSPEGQNDLcurated22369663; 25315811
327SRFDRNATVNNSAPIPhosphoGRID 2.0;curated19779198; 22817900
373KFPASSRYAPSPSPPcurated22817900
376ASSRYAPSPSPPASHdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19684113;
378SRYAPSPSPPASHISdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19684113;
382PSPSPPASHISRQAQPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;UniProt;curated21177495; 19779198; 21081558; 19060867; 22817900; 19795423
Sequence
(Fasta)		MSFRRINSRY FENRKGSSME EKKAKVPPNV NLSLWRKNTV ESDVHRFNSL PSKISGALTR 60
EQIYSYQVMF RIQEITIKLR TNDFVPPSRK NRSPSPPPVY DAQGKRTNTR EQRYRKKLED 120
ERIKLVEIAL KTIPYFVPPD DYKRPTKFQD KYYIPVDQYP DVNFVGLLLG PRGRTLRKLQ 180
EDSNCKIAIR GRGSVKEGKN ASDLPPGAMN FEDPLHCLII ADSEDKIQKG IKVCQNIVIK 240
AVTSPEGQND LKRGQLRELA ELNGTLREDN RPCPICGLKD HKRYDCPNRK IPNIQGIVCK 300
ICGQTGHFSR DCNSSSQRMS RFDRNATVNN SAPIQSNDVH YNSNTHPIQA PKRSRYDNNS 360
TEPPLKFPAS SRYAPSPSPP ASHISRQAQN VTPTPPPGLT SSSFSSGVPG IAPPPLQSPP 420
ESEQPKFSLP PPPGMTTVQS SIAPPPGLSG PPGFSNNMGN DINKPTPPGL QGPPGL 477
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0479--Metal-binding
KW-0507--mRNA processing
KW-0508--mRNA splicing
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0694--RNA-binding
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR031150--BBP/SF1
IPR013041--Coatomer/clathrin_app_Ig-like
IPR004087--KH_dom
IPR004088--KH_dom_type_1
IPR032570--SF1-HH
IPR001878--Znf_CCHC
PROSITE

PS50158--ZF_CCHC
Pfam

PF00013--KH_1
PF16275--SF1-HH
PF00098--zf-CCHC
Gene Ontology

GO:0000243--C:commitment complex
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0045131--F:pre-mRNA branch point binding
GO:0008270--F:zinc ion binding
GO:0000398--P:mRNA splicing, via spliceosome