dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0016511
Uniprot Accession Q12142; ATG9_YEAST; D6VRJ9;
Genbank Protein ID NP_010132.1;
Genbank Nucleotide ID NM_001180209.1;
Protein Name Autophagy-related protein 9
Protein Synonyms/Alias Cytoplasm to vacuole targeting protein 7;
Gene Name ATG9
Gene Synonyms/Alias APG9;AUT9;CVT7;YDL149W;D1560;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Required for mitophagy. Cycles between the PAS and the cytoplasmic vesicle pool and may participate in supplying membrane for the growing autophagosome. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking. Anterograde trafficking requires an intact actin cytoskeleton and proper retrograde trafficking of from the PAS to the cytoplasmic vesicles requires the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. Recruits ATG23 and ATG8 to the PAS.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
11DEYQLPNSHGKNTFLcurated25315811
59LLHSTNDSREDVDENcurated25315811
111DHQLNSVTSKENVLEdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
112HQLNSVTSKENVLETdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
119SKENVLETEKSNLERcurated22817900; 19547744
131LERLVEGSTDDSVPKPHOSIDA;curated21081558; 22369663; 22817900; 19795423; 24961812; 25315811
132ERLVEGSTDDSVPKVPHOSIDA;curated21081558; 22817900; 19795423
135VEGSTDDSVPKVGQLPHOSIDA;curated21081558; 22369663; 22817900; 19795423; 24961812; 25315811
143VPKVGQLSSEEEEDNPhosphoGRID 2.0;UniProt;curated19779198; 22369663; 22817900; 20377248
144PKVGQLSSEEEEDNEPhosphoGRID 2.0;UniProt;curated19779198; 22369663; 22817900; 20377248
236NGNQKHGTKYFGSATcurated22817900; 19547744
250TQPRFTGSPLNNTNRPhosphoGRID 2.0;curated19779198; 22369663; 22817900; 25315811
785KNIDGEDTHSMDEDScurated22369663; 22817900; 19547744
787IDGEDTHSMDEDSLTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
802KKIAVNGSHTLNSKRPhosphoPep 2.019060867
864YPNNEGDSFLNNKYScurated25315811
948HEYVLTESFLDSGAFcurated24961812
969DHNKMLNSNYNGNGIcurated22369663; 24961812
971NKMLNSNYNGNGILNcurated22369663
Sequence
(Fasta)		MERDEYQLPN SHGKNTFLSR IFGLQSDEVN PSLNSQEMSN FPLPDIERGS SLLHSTNDSR 60
EDVDENDLRV PESDQGTSTE EEDEVDEEQV QAYAPQISDG LDGDHQLNSV TSKENVLETE 120
KSNLERLVEG STDDSVPKVG QLSSEEEEDN EFINNDGFDD DTPLFQKSKI HEFSSKKSNT 180
IEDGKRPLFF RHILQNNRPQ RDTQKLFTSS NAIHHDKDKS ANNGPRNING NQKHGTKYFG 240
SATQPRFTGS PLNNTNRFTK LFPLRKPNLL SNISVLNNTP EDRINTLSVK ERALWKWANV 300
ENLDIFLQDV YNYYLGNGFY CIILEKILNI CTLLFVVFVS TYMGHCVDYS KLPTSHRVSD 360
IIIDKCYSNS ITGFTKFFLW MFYFFVILKI VQLYFDVQKL SELQNFYKYL LNISDDELQT 420
LPWQNVIQQL MYLKDQNAMT ANVVEVKAKN RIDAHDVANR IMRRENYLIA LYNSDILNLS 480
LPIPLFRTNV LTKTLEWNIN LCVMGFVFNE SGFIKQSILK PSQREFTREE LQKRFMLAGF 540
LNIILAPFLV TYFVLLYFFR YFNEYKTSPG SIGARQYTPI AEWKFREYNE LYHIFKKRIS 600
LSTTLANKYV DQFPKEKTNL FLKFVSFICG SFVAILAFLT VFDPENFLNF EITSDRSVIF 660
YITILGAIWS VSRNTITQEY HVFDPEETLK ELYEYTHYLP KEWEGRYHKE EIKLEFCKLY 720
NLRIVILLRE LTSLMITPFV LWFSLPSSAG RIVDFFRENS EYVDGLGYVC KYAMFNMKNI 780
DGEDTHSMDE DSLTKKIAVN GSHTLNSKRR SKFTAEDHSD KDLANNKMLQ SYVYFMDDYS 840
NSENLTGKYQ LPAKKGYPNN EGDSFLNNKY SWRKQFQPGQ KPELFRIGKH ALGPGHNISP 900
AIYSTRNPGK NWDNNNNGDD IKNGTNNATA KNDDNNGNND HEYVLTESFL DSGAFPNHDV 960
IDHNKMLNSN YNGNGILNKG GVLGLVKEYY KKSDVGR 998
Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0968--Cytoplasmic vesicle
KW-0256--Endoplasmic reticulum
KW-0333--Golgi apparatus
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0813--Transport
Interpro

IPR007241--Autophagy-rel_prot_9
PROSITE
Pfam

PF04109--APG9
Gene Ontology

GO:0005776--C:autophagosome
GO:0030659--C:cytoplasmic vesicle membrane
GO:0005789--C:endoplasmic reticulum membrane
GO:0000139--C:Golgi membrane
GO:0016021--C:integral component of membrane
GO:0005739--C:mitochondrion
GO:0000407--C:pre-autophagosomal structure
GO:0034045--C:pre-autophagosomal structure membrane
GO:0000045--P:autophagosome assembly
GO:0006914--P:autophagy
GO:0032258--P:CVT pathway
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0051260--P:protein homooligomerization
GO:0034497--P:protein localization to pre-autophagosomal structure
GO:0061709--P:reticulophagy