dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0014487
Uniprot Accession Q01279; EGFR_MOUSE;
Genbank Protein ID NP_997538.1;
Genbank Nucleotide ID NM_207655.2;
Protein Name Epidermal growth factor receptor
Protein Synonyms/Alias
Gene Name Egfr
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Phosphorylation Sites
 dbPAF PTMs: 30 (View all)
PositionPeptidesSourceReferences ( PMIDs )
270TCPPLMLYNPTTYQMdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated15016810; 16381945; 23193290; 22135298; 22817900
316RACGPDYYEVEEDGIdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;SysPTM 2.0;curated15378723; 16381945; 23193290; 22135298; 19366988; 22817900
695RELVEPLTPSGEAPNdbPTM 3.0;PhosphoSitePlus;curated22028470; 16381945; 23193290; 22135298; 20688971; 23567750;
729SGAFGTVYKGLWIPEPhosphoSitePlus22135298
871LGAEEKEYHAEGGKVdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;SysPTM 2.0;curated14647453; 16381945; 23193290; 17043637; 17139251; 18255094;
993DERMHLPSPTDSNFYdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated18515860; 21183079; 16381945; 23193290; 17242355; 19060867;
995RMHLPSPTDSNFYRAdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 22817900
997HLPSPTDSNFYRALMdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;curated21183079; 16381945; 23193290; 17242355; 22135298; 19366988;
1000SPTDSNFYRALMDEEdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;curated18515860; 16381945; 23193290; 22135298; 19366988; 22817900
1018DVVDADEYLIPQQGFdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;SysPTM 2.0;curated16123117; 16381945; 23193290; 17139251; 18515860; 19878607;
1028PQQGFFNSPSTSRTPcurated21743459
1034NSPSTSRTPLLSSLSPhosphoSitePlus;curated22135298; 23567750
1041TPLLSSLSATSNNSTPhosphoSitePlus22135298
1043LLSSLSATSNNSTVAdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;curated17242355; 16381945; 23193290; 22135298; 19366988; 23567750
1044LSSLSATSNNSTVACPhosphoSitePlus;curated22135298; 21743459; 23567750
1047LSATSNNSTVACINRdbPTM 3.0;SysPTM 2.0;curated17242355; 16381945; 23193290; 19366988; 21743459; 23567750
1057ACINRNGSCRVKEDAPhosphoSitePlus;curated22135298; 23984901
1069EDAFLQRYSSDPTGAdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;SysPTM 2.0;curated10635327; 16381945; 23193290; 12446705; 16123117; 17139251;
1070DAFLQRYSSDPTGAVdbPTM 3.0;PhosphoSitePlus;curated18515860; 19878607; 16381945; 23193290; 22135298; 22817900;
1071AFLQRYSSDPTGAVTdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;curated18515860; 19878607; 16381945; 23193290; 22135298; 19366988;
Sequence
(Fasta)		MRPSGTARTT LLVLLTALCA AGGALEEKKV CQGTSNRLTQ LGTFEDHFLS LQRMYNNCEV 60
VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN ALYENTYALA 120
ILSNYGTNRT GLRELPMRNL QEILIGAVRF SNNPILCNMD TIQWRDIVQN VFMSNMSMDL 180
QSHPSSCPKC DPSCPNGSCW GGGEENCQKL TKIICAQQCS HRCRGRSPSD CCHNQCAAGC 240
TGPRESDCLV CQKFQDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV 300
VTDHGSCVRA CGPDYYEVEE DGIRKCKKCD GPCRKVCNGI GIGEFKDTLS INATNIKHFK 360
YCTAISGDLH ILPVAFKGDS FTRTPPLDPR ELEILKTVKE ITGFLLIQAW PDNWTDLHAF 420
ENLEIIRGRT KQHGQFSLAV VGLNITSLGL RSLKEISDGD VIISGNRNLC YANTINWKKL 480
FGTPNQKTKI MNNRAEKDCK AVNHVCNPLC SSEGCWGPEP RDCVSCQNVS RGRECVEKCN 540
ILEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGIM 600
GENNTLVWKY ADANNVCHLC HANCTYGCAG PGLQGCEVWP SGPKIPSIAT GIVGGLLFIV 660
VVALGIGLFM RRRHIVRKRT LRRLLQEREL VEPLTPSGEA PNQAHLRILK ETEFKKIKVL 720
GSGAFGTVYK GLWIPEGEKV KIPVAIKELR EATSPKANKE ILDEAYVMAS VDNPHVCRLL 780
GICLTSTVQL ITQLMPYGCL LDYVREHKDN IGSQYLLNWC VQIAKGMNYL EDRRLVHRDL 840
AARNVLVKTP QHVKITDFGL AKLLGAEEKE YHAEGGKVPI KWMALESILH RIYTHQSDVW 900
SYGVTVWELM TFGSKPYDGI PASDISSILE KGERLPQPPI CTIDVYMIMV KCWMIDADSR 960
PKFRELILEF SKMARDPQRY LVIQGDERMH LPSPTDSNFY RALMDEEDME DVVDADEYLI 1020
PQQGFFNSPS TSRTPLLSSL SATSNNSTVA CINRNGSCRV KEDAFLQRYS SDPTGAVTED 1080
NIDDAFLPVP EYVNQSVPKR PAGSVQNPVY HNQPLHPAPG RDLHYQNPHS NAVGNPEYLN 1140
TAQPTCLSSG FNSPALWIQK GSHQMSLDNP DYQQDFFPKE TKPNGIFKGP TAENAEYLRV 1200
APPSSEFIGA 1211
Keyword

KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0217--Developmental protein
KW-1015--Disulfide bond
KW-0256--Endoplasmic reticulum
KW-0967--Endosome
KW-0325--Glycoprotein
KW-0333--Golgi apparatus
KW-1017--Isopeptide bond
KW-0418--Kinase
KW-0472--Membrane
KW-0488--Methylation
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0675--Receptor
KW-1185--Reference proteome
KW-0677--Repeat
KW-0732--Signal
KW-0808--Transferase
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0829--Tyrosine-protein kinase
KW-0832--Ubl conjugation
Interpro

IPR006211--Furin-like_Cys-rich_dom
IPR006212--Furin_repeat
IPR009030--Growth_fac_rcpt_N_dom
IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR000494--Rcpt_L-dom
IPR001245--Ser-Thr/Tyr_kinase_cat_dom
IPR008266--Tyr_kinase_AS
IPR020635--Tyr_kinase_cat_dom
IPR016245--Tyr_kinase_EGF/ERB/XmrK_rcpt
PROSITE

PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00109--PROTEIN_KINASE_TYR
Pfam

PF00757--Furin-like
PF07714--Pkinase_Tyr
PF01030--Recep_L_domain
Gene Ontology

GO:0016323--C:basolateral plasma membrane
GO:0009986--C:cell surface
GO:0005737--C:cytoplasm
GO:0031901--C:early endosome membrane
GO:0030139--C:endocytic vesicle
GO:0005789--C:endoplasmic reticulum membrane
GO:0005768--C:endosome
GO:0010008--C:endosome membrane
GO:0005925--C:focal adhesion
GO:0000139--C:Golgi membrane
GO:0016021--C:integral component of membrane
GO:0005622--C:intracellular
GO:0016020--C:membrane
GO:0045121--C:membrane raft
GO:0097489--C:multivesicular body, internal vesicle lumen
GO:0031965--C:nuclear membrane
GO:0005634--C:nucleus
GO:0048471--C:perinuclear region of cytoplasm
GO:0005886--C:plasma membrane
GO:0043235--C:receptor complex
GO:0051015--F:actin filament binding
GO:0005524--F:ATP binding
GO:0003682--F:chromatin binding
GO:0019899--F:enzyme binding
GO:0005006--F:epidermal growth factor-activated receptor activity
GO:0042802--F:identical protein binding
GO:0016301--F:kinase activity
GO:0030235--F:nitric-oxide synthase regulator activity
GO:0046982--F:protein heterodimerization activity
GO:0019903--F:protein phosphatase binding
GO:0004713--F:protein tyrosine kinase activity
GO:0004716--F:receptor signaling protein tyrosine kinase activity
GO:0004871--F:signal transducer activity
GO:0004888--F:transmembrane signaling receptor activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0000902--P:cell morphogenesis
GO:0008283--P:cell proliferation
GO:0007166--P:cell surface receptor signaling pathway
GO:0071230--P:cellular response to amino acid stimulus
GO:0071364--P:cellular response to epidermal growth factor stimulus
GO:0071392--P:cellular response to estradiol stimulus
GO:0021795--P:cerebral cortex cell migration
GO:0048546--P:digestive tract morphogenesis
GO:0001892--P:embryonic placenta development
GO:0007173--P:epidermal growth factor receptor signaling pathway
GO:0008544--P:epidermis development
GO:0001942--P:hair follicle development
GO:0007611--P:learning or memory
GO:0060571--P:morphogenesis of an epithelial fold
GO:0043066--P:negative regulation of apoptotic process
GO:0042177--P:negative regulation of protein catabolic process
GO:0018108--P:peptidyl-tyrosine phosphorylation
GO:0035413--P:positive regulation of catenin import into nucleus
GO:0030335--P:positive regulation of cell migration
GO:0008284--P:positive regulation of cell proliferation
GO:0031659--P:positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
GO:0045739--P:positive regulation of DNA repair
GO:0045740--P:positive regulation of DNA replication
GO:0050679--P:positive regulation of epithelial cell proliferation
GO:0070374--P:positive regulation of ERK1 and ERK2 cascade
GO:0048146--P:positive regulation of fibroblast proliferation
GO:0043406--P:positive regulation of MAP kinase activity
GO:0045429--P:positive regulation of nitric oxide biosynthetic process
GO:0042327--P:positive regulation of phosphorylation
GO:0051897--P:positive regulation of protein kinase B signaling
GO:0001934--P:positive regulation of protein phosphorylation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0046777--P:protein autophosphorylation
GO:0042127--P:regulation of cell proliferation
GO:0050999--P:regulation of nitric-oxide synthase activity
GO:0050730--P:regulation of peptidyl-tyrosine phosphorylation
GO:0070141--P:response to UV-A
GO:0007435--P:salivary gland morphogenesis
GO:0007165--P:signal transduction
GO:0016337--P:single organismal cell-cell adhesion