dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0006637
Uniprot Accession P19880; YAP1_YEAST; D6VZG8; P22631; Q06840;
Genbank Protein ID NP_013707.1;
Genbank Nucleotide ID NM_001182362.1;
Protein Name AP-1-like transcription factor YAP1
Protein Synonyms/Alias Phenanthroline resistance protein PAR1;Pleiotropic drug resistance protein PDR4;
Gene Name YAP1
Gene Synonyms/Alias PAR1;PDR4;SNQ3;YML007W;YM9571.12;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Transcription activator involved in oxidative stress response and redox homeostasis. Regulates the transcription of genes encoding antioxidant enzymes and components of the cellular thiol-reducing pathways, including the thioredoxin system (TRX2, TRR1), the glutaredoxin system (GSH1, GLR1), superoxide dismutase (SOD1, SOD2), glutathione peroxidase (GPX2), and thiol-specific peroxidases (TSA1, AHP1). The induction of some of these genes requires the cooperative action of both, YAP1 and SKN7. YAP1 preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of YAP1 is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Oxidative stress (as well as carbon stress, but not increased temperature, acidic pH, or ionic stress) induces nuclear accumulation and as a result YAP1 transcriptional activity. Nuclear export is restored when disulfide bonds are reduced by thioredoxin (TRX2), whose expression is controlled by YAP1, providing a mechanism for negative autoregulation. When overexpressed, YAP1 confers pleiotropic drug-resistance and increases cellular tolerance to cadmium, iron chelators and zinc.
Phosphorylation Sites
dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
9SVSTAKRSLDVVSPGPhosphoGRID 2.0;UniProt;curated19779198; 22369663; 22817900
14KRSLDVVSPGSLAEFdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17330950; 18407956; 16381945; 23193290; 21177495;
17LDVVSPGSLAEFEGSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900;
24SLAEFEGSKSRHDEIcurated22817900
135RPETRNDSKVLEYLAPhosphoGRID 2.0;curated19779198; 22817900
160NNVNHSNSEPIDTPNcurated24961812
165SNSEPIDTPNDDIQEPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated21177495; 19823750; 19795423; 19060867; 19779198; 26040289;
204NVGKQLPSPNDPSHSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 19823750;
209LPSPNDPSHSAPMPIcurated22817900
211SPNDPSHSAPMPINQPhosphoGRID 2.0;curated21177495; 19779198; 26040289; 25315811; 22817900
273FVSGDDGSNSKTKNLcurated25315811
372DATKYENSFSGFGRLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
394HYVVNDNSTGSTDSTPhosphoGRID 2.019779198
395YVVNDNSTGSTDSTGPhosphoGRID 2.019779198
397VNDNSTGSTDSTGSTPhosphoGRID 2.0;PhosphoPep 2.019779198; 19060867
398NDNSTGSTDSTGSTGPhosphoGRID 2.0;curated19779198; 22817900
403GSTDSTGSTGNKNKKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988
404STDSTGSTGNKNKKNPhosphoGRID 2.019779198
503YDMFFRDSSKEGNNLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19366988; 26040289;
504DMFFRDSSKEGNNLFdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 18407956; 16381945; 23193290; 19366988; 22369663;
Sequence
(Fasta)
MSVSTAKRSL DVVSPGSLAE FEGSKSRHDE IENEHRRTGT RDGEDSEQPK KKGSKTSKKQ 60
DLDPETKQKR TAQNRAAQRA FRERKERKMK ELEKKVQSLE SIQQQNEVEA TFLRDQLITL 120
VNELKKYRPE TRNDSKVLEY LARRDPNLHF SKNNVNHSNS EPIDTPNDDI QENVKQKMNF 180
TFQYPLDNDN DNDNSKNVGK QLPSPNDPSH SAPMPINQTQ KKLSDATDSS SATLDSLSNS 240
NDVLNNTPNS STSMDWLDNV IYTNRFVSGD DGSNSKTKNL DSNMFSNDFN FENQFDEQVS 300
EFCSKMNQVC GTRQCPIPKK PISALDKEVF ASSSILSSNS PALTNTWESH SNITDNTPAN 360
VIATDATKYE NSFSGFGRLG FDMSANHYVV NDNSTGSTDS TGSTGNKNKK NNNNSDDVLP 420
FISESPFDMN QVTNFFSPGS TGIGNNAASN TNPSLLQSSK EDIPFINANL AFPDDNSTNI 480
QLQPFSESQS QNKFDYDMFF RDSSKEGNNL FGEFLEDDDD DKKAANMSDD ESSLIKNQLI 540
NEEPELPKQY LQSVPGNESE ISQKNGSSLQ NADKINNGND NDNDNDVVPS KEGSLLRCSE 600
IWDRITTHPK YSDIDVDGLC SELMAKAKCS ERGVVINAED VQLALNKHMN 651
Keyword

KW-0002--3D-structure
KW-0010--Activator
KW-0105--Cadmium resistance
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-1015--Disulfide bond
KW-0238--DNA-binding
KW-0539--Nucleus
KW-0558--Oxidation
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation

Interpro

IPR004827--bZIP
IPR013910--TF_PAP1
IPR023167--Yap1_redox_dom

PROSITE

PS50217--BZIP
PS00036--BZIP_BASIC

Pfam

PF00170--bZIP_1
PF08601--PAP1

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0043565--F:sequence-specific DNA binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0043619--P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0046686--P:response to cadmium ion
GO:0042493--P:response to drug
GO:0009408--P:response to heat
GO:0010038--P:response to metal ion
GO:0000304--P:response to singlet oxygen
GO:0006351--P:transcription, DNA-templated