dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006618
Uniprot Accession P19659; MED15_YEAST; D6W217; Q08221;
Genbank Protein ID NP_014591.1;
Genbank Nucleotide ID NM_001183305.1;
Protein Name Mediator of RNA polymerase II transcription subunit 15
Protein Synonyms/Alias Autonomous replication regulatory protein 3;Basal expression activator protein 1;Defective silencing suppressor protein 4;Mediator complex subunit 15;Transcription regulatory protein GAL11;Ty insertion suppressor protein 13;
Gene Name GAL11
Gene Synonyms/Alias ABE1;MED15;RAR3;SDS4;SPT13;YOL051W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.
Phosphorylation Sites
 dbPAF PTMs: 49 (View all)
PositionPeptidesSourceReferences ( PMIDs )
13VQDKDTLSNAERAKNcurated24961812
80KVAVMRNTYNTRKNAcurated22817900
163QQQRRQLTPQQQQLVdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 22369663;
335MTEPVKQSFIRKYINdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
728PYMNHKNTSSMDFLNcurated25315811
729YMNHKNTSSMDFLNSPhosphoGRID 2.0;curated20702584; 19823750; 25315811
730MNHKNTSSMDFLNSMdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 22369663;
736SSMDFLNSMENTPKVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988; 24961812
740FLNSMENTPKVPVSAPhosphoPep 2.0;curated19060867; 25315811
746NTPKVPVSAAATPSLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 26040289; 22817900;
750VPVSAAATPSLNKTIdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 26040289; 22817900;
752VSAAATPSLNKTINGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
765NGKVNGRTKSNTIPVPhosphoPep 2.0;curated19060867; 26040289; 22817900
767KVNGRTKSNTIPVTSPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19779198; 19795423; 19060867; 26040289; 22369663;
769NGRTKSNTIPVTSIPPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19795423; 19060867; 26040289; 22817900; 24961812;
783PSTNKKLSISNAASQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 20702584; 19823750;
785TNKKLSISNAASQQPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 20702584; 19684113;
789LSISNAASQQPTPRSPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated19684113; 19779198; 19060867; 26040289; 22369663; 22817900;
793NAASQQPTPRSASNTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19684113; 19779198; 19060867;
796SQQPTPRSASNTAKSPhosphoGRID 2.0;curated19684113; 26040289; 22369663; 22817900; 19823750; 24961812
Sequence
(Fasta)		MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK NFEAALFAKS 60
SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV EQHHINNLKN SGNSANNMNV 120
NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK 180
QLLQRIPNIP PNINTWQQVT ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ 240
AQANNNNNGL PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF 300
SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ ALRDVKNNNN 360
ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN AAAFSQQQNA SSKLYQMQQQ 420
QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ 480
AHAQHQPSQQ PQQAQQQPNP LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK 540
EKIKMKLKQG QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL 600
DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN NIATQQNMQQ 660
SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP SSTPGVANYS AMANAPGNNI 720
PYMNHKNTSS MDFLNSMENT PKVPVSAAAT PSLNKTINGK VNGRTKSNTI PVTSIPSTNK 780
KLSISNAASQ QPTPRSASNT AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN 840
SAIIENAFRK EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML 900
TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN KSNRSYSIAL 960
SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR KASVLEISPQ DSIASVLSPD 1020
SNIMSDSKKI KVDSPDDPFM TKSGATTSEK QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA 1080
T 1082
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0010--Activator
KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR003101--KIX_dom
IPR008626--Mediator_Med15_fun
PROSITE
Pfam

PF16987--KIX_2
PF05397--Med15_fungi
Gene Ontology

GO:0070847--C:core mediator complex
GO:0016592--C:mediator complex
GO:0001102--F:RNA polymerase II activating transcription factor binding
GO:0001128--F:RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly
GO:0001095--F:TFIIE-class transcription factor binding
GO:0001097--F:TFIIH-class transcription factor binding
GO:0001135--F:transcription factor activity, RNA polymerase II transcription factor recruiting
GO:0001088--F:transcription factor activity, TFIIE-class binding
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:2000219--P:positive regulation of invasive growth in response to glucose limitation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0051123--P:RNA polymerase II transcriptional preinitiation complex assembly