dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006599
Uniprot Accession P19524; MYO2_YEAST; D6W323;
Genbank Protein ID NP_014971.1;
Genbank Nucleotide ID NM_001183746.1;
Protein Name Myosin-2
Protein Synonyms/Alias Cell division control protein 66;Class V unconventional myosin MYO2;Type V myosin heavy chain MYO2;Myosin V MYO2;
Gene Name MYO2
Gene Synonyms/Alias CDC66;YOR326W;O6167;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis.
Phosphorylation Sites
 dbPAF PTMs: 17
PositionPeptidesSourceReferences ( PMIDs )
545LYQTLDKSPTNKVFSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 17330950; 19060867;
907FLRTKKDTVVVQSLIPhosphoGRID 2.021126336
1097TTSVLPQTPLKDVMGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988; 22817900; 24961812;
1118NNMMLENSDLSPNDLcurated19823750; 25521595
1121MLENSDLSPNDLNLKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19823750; 19795423; 19060867;
1129PNDLNLKSRSTPSSGPhosphoPep 2.019060867
1131DLNLKSRSTPSSGNNPhosphoGRID 2.0;PHOSIDA;curated21177495; 20702584; 19779198; 21081558; 25704821; 22817900;
1132LNLKSRSTPSSGNNHPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 16467380; 19779198; 19060867; 26040289; 22817900;
1134LKSRSTPSSGNNHIDPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 16467380; 19779198; 19060867; 25704821; 22817900;
1135KSRSTPSSGNNHIDSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 16467380; 20702584;
1144NNHIDSLSVDRENGVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
1221RFGLTKQSESFLAQVPhosphoGRID 2.0;curated19823750; 19795423; 22817900
1223GLTKQSESFLAQVLTPhosphoGRID 2.0;curated19823750; 19795423; 22817900
1231FLAQVLTTIQKVVTQPhosphoGRID 2.0;curated19823750; 19795423
1364DILTFFNSIYWCMKScurated22817900
1507KKEAALSSSGNDSKGcurated25315811
1508KEAALSSSGNDSKGHcurated25315811
Sequence
(Fasta)		MSFEVGTRCW YPHKELGWIG AEVIKNEFND GKYHLELQLE DDEIVSVDTK DLNNDKDQSL 60
PLLRNPPILE ATEDLTSLSY LNEPAVLHAI KQRYSQLNIY TYSGIVLIAT NPFDRVDQLY 120
TQDMIQAYAG KRRGELEPHL FAIAEEAYRL MKNDKQNQTI VVSGESGAGK TVSAKYIMRY 180
FASVEEENSA TVQHQVEMSE TEQKILATNP IMEAFGNAKT TRNDNSSRFG KYLEILFDKD 240
TSIIGARIRT YLLERSRLVY QPPIERNYHI FYQLMAGLPA QTKEELHLTD ASDYFYMNQG 300
GDTKINGIDD AKEYKITVDA LTLVGITKET QHQIFKILAA LLHIGNIEIK KTRNDASLSA 360
DEPNLKLACE LLGIDAYNFA KWVTKKQIIT RSEKIVSNLN YSQALVAKDS VAKFIYSALF 420
DWLVENINTV LCNPAVNDQI SSFIGVLDIY GFEHFEKNSF EQFCINYANE KLQQEFNQHV 480
FKLEQEEYVK EEIEWSFIEF NDNQPCIDLI ENKLGILSLL DEESRLPAGS DESWTQKLYQ 540
TLDKSPTNKV FSKPRFGQTK FIVSHYALDV AYDVEGFIEK NRDTVSDGHL EVLKASTNET 600
LINILEGLEK AAKKLEEAKK LELEQAGSKK PGPIRTVNRK PTLGSMFKQS LIELMNTINS 660
TNVHYIRCIK PNADKEAWQF DNLMVLSQLR ACGVLETIRI SCAGFPSRWT FEEFVLRYYI 720
LIPHEQWDLI FKKKETTEED IISVVKMILD ATVKDKSKYQ IGNTKIFFKA GMLAYLEKLR 780
SNKMHNSIVM IQKKIRAKYY RKQYLQISQA IKYLQNNIKG FIIRQRVNDE MKVNCATLLQ 840
AAYRGHSIRA NVFSVLRTIT NLQKKIRKEL KQRQLKQEHE YNAAVTIQSK VRTFEPRSRF 900
LRTKKDTVVV QSLIRRRAAQ RKLKQLKADA KSVNHLKEVS YKLENKVIEL TQNLASKVKE 960
NKEMTERIKE LQVQVEESAK LQETLENMKK EHLIDIDNQK SKDMELQKTI ENNLQSTEQT 1020
LKDAQLELED MVKQHDELKE ESKKQLEELE QTKKTLVEYQ TLNGDLQNEV KSLKEEIARL 1080
QTAMSLGTVT TSVLPQTPLK DVMGGGASNF NNMMLENSDL SPNDLNLKSR STPSSGNNHI 1140
DSLSVDRENG VNATQINEEL YRLLEDTEIL NQEITEGLLK GFEVPDAGVA IQLSKRDVVY 1200
PARILIIVLS EMWRFGLTKQ SESFLAQVLT TIQKVVTQLK GNDLIPSGVF WLANVRELYS 1260
FVVFALNSIL TEETFKNGMT DEEYKEYVSL VTELKDDFEA LSYNIYNIWL KKLQKQLQKK 1320
AINAVVISES LPGFSAGETS GFLNKIFANT EEYTMDDILT FFNSIYWCMK SFHIENEVFH 1380
AVVTTLLNYV DAICFNELIM KRNFLSWKRG LQLNYNVTRL EEWCKTHGLT DGTECLQHLI 1440
QTAKLLQVRK YTIEDIDILR GICYSLTPAQ LQKLISQYQV ADYESPIPQE ILRYVADIVK 1500
KEAALSSSGN DSKGHEHSSS IFITPETGPF TDPFSLIKTR KFDQVEAYIP AWLSLPSTKR 1560
IVDLVAQQVV QDGH 1575
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0009--Actin-binding
KW-0067--ATP-binding
KW-0112--Calmodulin-binding
KW-0131--Cell cycle
KW-0132--Cell division
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0505--Motor protein
KW-0518--Myosin
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0677--Repeat
KW-0813--Transport
Interpro

IPR002710--Dilute_dom
IPR000048--IQ_motif_EF-hand-BS
IPR001609--Myosin_head_motor_dom
IPR008989--Myosin_S1_N
IPR027417--P-loop_NTPase
PROSITE

PS51126--DILUTE
PS50096--IQ
PS51456--MYOSIN_MOTOR
Pfam

PF01843--DIL
PF00063--Myosin_head
Gene Ontology

GO:0032432--C:actin filament bundle
GO:0005935--C:cellular bud neck
GO:0005934--C:cellular bud tip
GO:0031941--C:filamentous actin
GO:0000131--C:incipient cellular bud site
GO:0043332--C:mating projection tip
GO:0071563--C:Myo2p-Vac17p-Vac8p transport complex
GO:0016459--C:myosin complex
GO:0030133--C:transport vesicle
GO:0031982--C:vesicle
GO:0051015--F:actin filament binding
GO:0005524--F:ATP binding
GO:0005516--F:calmodulin binding
GO:0000146--F:microfilament motor activity
GO:0007118--P:budding cell apical bud growth
GO:0000132--P:establishment of mitotic spindle orientation
GO:0048313--P:Golgi inheritance
GO:0007107--P:membrane addition at site of cytokinesis
GO:0000001--P:mitochondrion inheritance
GO:0045033--P:peroxisome inheritance
GO:0015031--P:protein transport
GO:0009826--P:unidimensional cell growth
GO:0000011--P:vacuole inheritance
GO:0030050--P:vesicle transport along actin filament
GO:0016192--P:vesicle-mediated transport