dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006583
Uniprot Accession P19414; ACON_YEAST; D6VYU7;
Genbank Protein ID NP_013407.1;
Genbank Nucleotide ID NM_001182192.1;
Protein Name Aconitate hydratase, mitochondrial
Protein Synonyms/Alias
Gene Name ACO1
Gene Synonyms/Alias GLU1;YLR304C;L8003.22;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. Can also provide minor contributions to the reversible dehydration of (R)-homocitrate to cis-homoaconitate, a step in the alpha-aminoadipate pathway for lysine biosynthesis. Plays also an essential role in mtDNA maintenance. May directly protect mtDNA from accumulation of point mutations and ssDNA breaks as a component of mitochondrial nucleoids, or by preventing accumulation of iron citrate thereby alleviating its detrimental effects in mitochondria.
Phosphorylation Sites
 dbPAF PTMs: 8
PositionPeptidesSourceReferences ( PMIDs )
83QDIQRGVSYLKLRPDcurated22817900; 24961812; 20377248
351PYINGPFTPDLATPVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
383VGLIGSCTNSSYEDMcurated22817900; 19547744
385LIGSCTNSSYEDMSRcurated22817900; 19547744
391NSSYEDMSRSASIVKUniProt;curated19779198; 22817900; 20377248; 19547744
409AHGLKSKTIFTVTPGdbPTM 3.0;PhosphoGRID 2.0;UniProt17761666; 16381945; 23193290
556STVEVKVSPTSDRLQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 16381945; 23193290; 19060867; 19366988; 22369663;
767IEWFKYGSALNKIKAPhosphoGRID 2.0;curated19779198; 22817900
Sequence
(Fasta)		MLSARSAIKR PIVRGLATVS NLTRDSKVNQ NLLEDHSFIN YKQNVETLDI VRKRLNRPFT 60
YAEKILYGHL DDPHGQDIQR GVSYLKLRPD RVACQDATAQ MAILQFMSAG LPQVAKPVTV 120
HCDHLIQAQV GGEKDLKRAI DLNKEVYDFL ASATAKYNMG FWKPGSGIIH QIVLENYAFP 180
GALIIGTDSH TPNAGGLGQL AIGVGGADAV DVMAGRPWEL KAPKILGVKL TGKMNGWTSP 240
KDIILKLAGI TTVKGGTGKI VEYFGDGVDT FSATGMGTIC NMGAEIGATT SVFPFNKSMI 300
EYLEATGRGK IADFAKLYHK DLLSADKDAE YDEVVEIDLN TLEPYINGPF TPDLATPVSK 360
MKEVAVANNW PLDVRVGLIG SCTNSSYEDM SRSASIVKDA AAHGLKSKTI FTVTPGSEQI 420
RATIERDGQL ETFKEFGGIV LANACGPCIG QWDRRDIKKG DKNTIVSSYN RNFTSRNDGN 480
PQTHAFVASP ELVTAFAIAG DLRFNPLTDK LKDKDGNEFM LKPPHGDGLP QRGYDAGENT 540
YQAPPADRST VEVKVSPTSD RLQLLKPFKP WDGKDAKDMP ILIKAVGKTT TDHISMAGPW 600
LKYRGHLENI SNNYMIGAIN AENKKANCVK NVYTGEYKGV PDTARDYRDQ GIKWVVIGDE 660
NFGEGSSREH AALEPRFLGG FAIITKSFAR IHETNLKKQG LLPLNFKNPA DYDKINPDDR 720
IDILGLAELA PGKPVTMRVH PKNGKPWDAV LTHTFNDEQI EWFKYGSALN KIKADEKK 779
Keyword

KW-0004--4Fe-4S
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0456--Lyase
KW-0479--Metal-binding
KW-0496--Mitochondrion
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0809--Transit peptide
KW-0816--Tricarboxylic acid cycle
Interpro

IPR015931--Acnase/IPM_dHydase_lsu_aba_1/3
IPR015937--Acoase/IPM_deHydtase
IPR001030--Acoase/IPM_deHydtase_lsu_aba
IPR015928--Aconitase/3IPM_dehydase_swvl
IPR015932--Aconitase/IPMdHydase_lsu_aba_2
IPR018136--Aconitase_4Fe-4S_BS
IPR006248--Aconitase_mito-like
IPR000573--AconitaseA/IPMdHydase_ssu_swvl
PROSITE

PS00450--ACONITASE_1
PS01244--ACONITASE_2
Pfam

PF00330--Aconitase
PF00694--Aconitase_C
Gene Ontology

GO:0005829--C:cytosol
GO:0005758--C:mitochondrial intermembrane space
GO:0005759--C:mitochondrial matrix
GO:0042645--C:mitochondrial nucleoid
GO:0051539--F:4 iron, 4 sulfur cluster binding
GO:0003994--F:aconitate hydratase activity
GO:0003690--F:double-stranded DNA binding
GO:0046872--F:metal ion binding
GO:0003697--F:single-stranded DNA binding
GO:0000002--P:mitochondrial genome maintenance
GO:0006099--P:tricarboxylic acid cycle