dbPAF Protein Information
| Tag | Content |
|---|---|
| dbPAF ID | dbPAF-0006568 |
| Uniprot Accession | P19263; MED14_YEAST; D6VY72; Q07999; |
| Genbank Protein ID | NP_013172.1; |
| Genbank Nucleotide ID | NM_001181958.1; |
| Protein Name | Mediator of RNA polymerase II transcription subunit 14 |
| Protein Synonyms/Alias | Glucose repression regulatory protein 1;Mediator complex subunit 14; |
| Gene Name | RGR1 |
| Gene Synonyms/Alias | MED14;YLR071C; |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID | 559292 |
| Functional Description (View all) |
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. |
| Phosphorylation Sites |
dbPAF PTMs: 3 |
| Sequence (Fasta) | MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE 60 SLEMVPKDTS AATMTSAPPP ALPHVEINQV SLALVIRNLT VFTMKELAQY MKTNVHTQAN 120 EPNSAKKIRF LQLIIFLRTQ FLKLYVLVKW TRTIKQNNFH VLIDLLNWFR TTNMNVNNCI 180 WALKSSLNSM TNAKLPNVDL VTALEVLSLG RPNLPTHNFK LSGVSNSMDM VDGMAKVPIG 240 LILQRLKDLN LTVSIKIALM NIPKPLNSYH IKNGRIYFTV PNEFEIQLST VNRQSPLFFV 300 DLKLLFNTEA EQTVSAVTEA TSTNGDSENN EENSSSNGNN LPLNKPRLEK LINEILLKSN 360 DPLLSLYNFL HKYVLTLQLY MVHREFLKLA NGGKFSKSNL IHNYDSKKST ITVRYWLNGK 420 MDSKGKITIG IQRTTESLIL KWDNQSASRA KNMPVIYNNI VSNIEGILDE IMFNHARIIR 480 SELLARDIFQ EDEENSDVLL FQLPTTCVSM APIQLKIDLL SGQFYFRNPT PLLSNYASKI 540 NRAEGPEELA RILQQLKLDK IIHVLTTMFE NTGWSCSRII KIDKPIRTQV NTGGESVVKK 600 EDNKYAIAGN STTNSDVSLL LQRDLFIRLP HWPLNWYLIL SIISSKTSCV VEKRIGKIVS 660 QRGKWNLKYL DNSNVMTVKL ESITYQKIMI LQRTILNRII NHMLIDSLNQ LEIRNKICSS 720 EMINEQKLPQ YIIQGSNTND NISIITLELE SFLEGSKALN SILESSMFLR IDYSNSQIRL 780 YAKFKRNTMM IQCQIDKLYI HFVQEEPLAF YLEESFTNLG IIVQYLTKFR QKLMQLVVLT 840 DVVERLHKNF ESENFKIIAL QPNEISFKYL SNNDEDDKDC TIKISTNDDS IKNLTVQLSP 900 SNPQHIIQPF LDNSKMDYHF IFSYLQFTSS LFKALKVILN ERGGKFHESG SQYSTMVNIG 960 LHNLNEYQIV YYNPQAGTKI TICIELKTVL HNGRDKIQFH IHFADVAHIT TKSPAYPMMH 1020 QVRNQVFMLD TKRLGTPESV KPANASHAIR LGNGVACDPS EIEPILMEIH NILKVDSNSS 1080 SS 1083 |
| Keyword | KW-0007--Acetylation |
| Interpro | IPR013947--Mediator_Med14 |
| PROSITE | |
| Pfam | PF08638--Med14 |
| Gene Ontology | GO:0070847--C:core mediator complex |