dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006529
Uniprot Accession P18963; IRA1_YEAST; D6VQD6;
Genbank Protein ID NP_009698.3;
Genbank Nucleotide ID NM_001178488.3;
Protein Name Inhibitory regulator protein IRA1
Protein Synonyms/Alias
Gene Name IRA1
Gene Synonyms/Alias GLC1;PPD1;YBR140C;YBR1016;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Inhibitory regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Stimulates the GTPase activity of Ras proteins.
Phosphorylation Sites
 dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
62IILNIAITKDLNPIIPhosphoGRID 2.0;curated19779198; 22817900
207QKRNYPAYTEKIDLTcurated25315811
385HSSSSSKTTNTNSPNcurated22817900
390SKTTNTNSPNSISKTPhosphoPep 2.0;curated19060867; 22817900; 25315811
393TNTNSPNSISKTSIKcurated22817900
395TNSPNSISKTSIKQScurated25315811
497NIPQRIFSLDDISSFdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22369663;
615MNINPKDSNTSSWGScurated22817900
617INPKDSNTSSWGSAScurated22817900
618NPKDSNTSSWGSASKcurated22817900
619PKDSNTSSWGSASKNcurated22817900
622SNTSSWGSASKNPSIcurated22817900
624TSSWGSASKNPSIRHcurated22817900
654RNVKFLTYLIRNLNGPhosphoGRID 2.019779198
892QSPLSINSGSNITNTdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
894PLSINSGSNITNTRTcurated22817900
915LPTLSNRSSASDLSLdbPTM 3.0;PhosphoGRID 2.0;UniProt;curated17563356; 16381945; 23193290; 19779198; 22817900
956PTTKRYASPNDSERScurated25315811
1332QELEKSKSAPDNEGDPhosphoGRID 2.021126336
1340APDNEGDSLSFESRNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900; 24961812
Sequence
(Fasta)		MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN SCRSIILNIA 60
ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV LLRLLSDVFE YYWDQNNDFK 120
KIRNDNYKPG FSSHRPNFHT SRPKHTRINP ALATMLLCKI SKLKFNTRTL KVLQNMSHHL 180
SGSATISKSS ILPDSQEFLQ KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA 240
PLLISHTSTE LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA 300
FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL LFDDVYSTFS 360
VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK QSSVNASGNV SPSQFSTGND 420
ASPTSPMASL SSPLNTNILG YPLSPITSTL GQANTSTSTT AATTKTDADT PSTMNTNNNN 480
NNNNSANLNN IPQRIFSLDD ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG 540
VNNSQSQNDQ SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY 600
RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK FLTYLIRNLN 660
GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS KRFYNLLGQN LEVGTNWNSA 720
TANTFISHCV ERNPLTHRRL QLEFFASGLQ LDSDLFLRHL QLEKELNHID LPKISLYTEG 780
FRVFFHLVST KKLHEDIAEK TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL 840
DQFDAARTLS NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR 900
TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT TKRYASPNDS 960
ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA TPTILKNIKS PKPNKTKKIA 1020
DDKQLKQPSY SRVILSDNDE ARKIMMNIFS IFKRMTNWFI RPDANTEFPK TFTDIIKPLF 1080
VSILDSNQRL QVTARAFIEI PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD 1140
LKLENAKREM LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS 1200
LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD NFTASGSVAL 1260
QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT QEELVDFRSL AGILASMSGI 1320
LSDMQELEKS KSAPDNEGDS LSFESRNPAY EVHKSLKLEL TKKMNFFISK QCQWLNNPNL 1380
LTRENSRDIL SIELHPLSFN LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI 1440
LKRDDDEKIM LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG 1500
ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY IDTSIESAKA 1560
LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL EKSADLNQFP VSLRHKISIL 1620
NENVIIALTN LSNANVNVSL KFTLPMGYSP NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK 1680
MLAIDDFLKY IIKNPILAFF GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK 1740
RAARSDDILR RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN 1800
SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY IAVGSFVFLR 1860
FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR ENIFKKDILV SKEEFLKTCS 1920
DKIFNFLSEL CKIPTNNFTV NVREDPTPIS FDYSFLHKFF YLNEFTIRKE IINESKLPGE 1980
FSFLKNTVML NDKILGVLGQ PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE 2040
EEDNAPFVHE AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT 2100
TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV ENPYLVTTIP 2160
RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD KEKKKFCPVS LKIGNKYFQV 2220
LHEIPQLYKV TVSNRTFSIK FNNVYKISNL ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP 2280
KYLEIVKMFY YAQLKMEEDF GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV 2340
GLFNEDDLVK NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST 2400
ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG PEAISRIIYS 2460
LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH ALDRDSENRD WMKAVSILTS 2520
FPTTEIACQV IEKLINMIKS FLPSLAVEAS AHSWSELTIL SKISVSIFFE SPLLSQMYLP 2580
EILFAVSLLI DVGPSEIRVS LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF 2640
ISGFSQEKGR VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA 2700
IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM FIIIAHVFTY 2760
SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM VNCLKRLYTV HLQLGFDGKS 2820
LAKKLMESRN FAATLLAKLE SYNGCIWNED NFPHIILGFI ANGLSIPVVK GAALDCLQAL 2880
FKNTYYERKS NPKSSDYLCY LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI 2940
NWLSSDSDKS NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF 3000
RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV VKPLDIFDQE 3060
HIEKLKGEGE HQVAIYERKR LATMILARMS CS 3093
Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0343--GTPase activation
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR023152--RasGAP_CS
IPR001936--RasGAP_dom
IPR008936--Rho_GTPase_activation_prot
PROSITE

PS00509--RAS_GTPASE_ACTIV_1
PS50018--RAS_GTPASE_ACTIV_2
Pfam

PF00616--RasGAP
Gene Ontology

GO:0005737--C:cytoplasm
GO:0031235--C:intrinsic component of the cytoplasmic side of the plasma membrane
GO:0016020--C:membrane
GO:0005096--F:GTPase activator activity
GO:0030818--P:negative regulation of cAMP biosynthetic process
GO:0046580--P:negative regulation of Ras protein signal transduction
GO:0043547--P:positive regulation of GTPase activity
GO:0045761--P:regulation of adenylate cyclase activity
GO:0007165--P:signal transduction