dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0006471
Uniprot Accession P18494; GLN3_YEAST; D3DLU0;
Genbank Protein ID NP_010958.3;
Genbank Nucleotide ID NM_001178931.3;
Protein Name Nitrogen regulatory protein GLN3
Protein Synonyms/Alias
Gene Name GLN3
Gene Synonyms/Alias YER040W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.
Phosphorylation Sites
dbPAF PTMs: 60 (View all)
PositionPeptidesSourceReferences ( PMIDs )
189LFPYNSSTSNSNINQcurated20377248
223YKLQNNNSSSSAMNIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 26040289;
224KLQNNNSSSSAMNITdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
225LQNNNSSSSAMNITNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 19823750;
226QNNNSSSSAMNITNNcurated22369663; 25315811; 20377248
249QHPFLKKSDSIGLSSdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 19779198; 22817900
251PFLKKSDSIGLSSSNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19060867; 19366988; 22369663;
255KSDSIGLSSSNTTNSPhosphoPep 2.019060867
256SDSIGLSSSNTTNSVcurated19823750; 25315811
257DSIGLSSSNTTNSVRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 24961812;
259IGLSSSNTTNSVRKNcurated20377248
262SSSNTTNSVRKNSLIPhosphoPep 2.0;curated19060867; 24961812
267TNSVRKNSLIKPMSSPhosphoGRID 2.0;UniProt;curated19779198; 25704821; 22369663; 22817900; 19823750; 24961812
273NSLIKPMSSTSLANFPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19795423; 19060867; 26040406; 22369663; 22817900;
274SLIKPMSSTSLANFKcurated19823750; 25315811
275LIKPMSSTSLANFKRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19684113; 19823750; 19795423;
276IKPMSSTSLANFKRAPhosphoGRID 2.0;curated19684113; 26040406; 22817900; 24961812; 25521595
285ANFKRAASVSSSISNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 21126336; 19779198; 19060867;
288KRAASVSSSISNMEPcurated22369663; 24961812
289RAASVSSSISNMEPSPhosphoPep 2.0;curated19060867; 22369663
Sequence
(Fasta)
MQDDPENSKL YDLLNSHLDV HGRSNEEPRQ TGDSRSQSSG NTGENEEDIA FASGLNGGTF 60
DSMLEALPDD LYFTDFVSPF TAAATTSVTT KTVKDTTPAT NHMDDDIAMF DSLATTQPID 120
IAASNQQNGE IAQLWDFNVD QFNMTPSNSS GSATISAPNS FTSDIPQYNH GSLGNSVSKS 180
SLFPYNSSTS NSNINQPSIN NNSNTNAQSH HSFNIYKLQN NNSSSSAMNI TNNNNSNNSN 240
IQHPFLKKSD SIGLSSSNTT NSVRKNSLIK PMSSTSLANF KRAASVSSSI SNMEPSGQNK 300
KPLIQCFNCK TFKTPLWRRS PEGNTLCNAC GLFQKLHGTM RPLSLKSDVI KKRISKKRAK 360
QTDPNIAQNT PSAPATASTS VTTTNAKPIR SRKKSLQQNS LSRVIPEEII RDNIGNTNNI 420
LNVNRGGYNF NSVPSPVLMN SQSYNSSNAN FNGASNANLN SNNLMRHNSN TVTPNFRRSS 480
RRSSTSSNTS SSSKSSSRSV VPILPKPSPN SANSQQFNMN MNLMNTTNNV SAGNSVASSP 540
RIISSANFNS NSPLQQNLLS NSFQRQGMNI PRRKMSRNAS YSSSFMAASL QQLHEQQQVD 600
VNSNTNTNSN RQNWNSSNSV STNSRSSNFV SQKPNFDIFN TPVDSPSVSR PSSRKSHTSL 660
LSQQLQNSES NSFISNHKFN NRLSSDSTSP IKYEADVSAG GKISEDNSTK GSSKESSAIA 720
DELDWLKFGI 731
Keyword

KW-0010--Activator
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0479--Metal-binding
KW-0534--Nitrate assimilation
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000679--Znf_GATA
IPR013088--Znf_NHR/GATA

PROSITE

PS00344--GATA_ZN_FINGER_1
PS50114--GATA_ZN_FINGER_2

Pfam

PF00320--GATA

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0005667--C:transcription factor complex
GO:0003682--F:chromatin binding
GO:0000978--F:RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0001228--F:transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0008270--F:zinc ion binding
GO:0030154--P:cell differentiation
GO:0042128--P:nitrate assimilation
GO:0001080--P:nitrogen catabolite activation of transcription from RNA polymerase II promoter
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0006366--P:transcription from RNA polymerase II promoter