dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006320
Uniprot Accession P17255; VATA_YEAST; D6VRG7; O74301; Q9Y7W5;
Genbank Protein ID NP_010096.1;
Genbank Nucleotide ID NM_001180245.1;
Protein Name V-type proton ATPase catalytic subunit A
Protein Synonyms/Alias
Gene Name VMA1
Gene Synonyms/Alias CLS8;TFP1;YDL185W;D1286;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase (vacuolar ATPase) is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mV, inside positive and acidic, in the vacuolar membrane vesicles. It may participate in maintenance of cytoplasmic Ca(2+) homeostasis. This is a catalytic subunit.
Phosphorylation Sites
 dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
16RKEIKRISLEDHAESdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
89VGDPVLRTGKPLSVEcurated22369663
94LRTGKPLSVELGPGLcurated22369663
122AIKEESQSIYIPRGIcurated22817900; 19547744
131YIPRGIDTPALDRTIdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 21126336; 19779198;
143RTIKWQFTPGKFQVGcurated22817900; 19547744
166YGSVFENSLISSHKIdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19366988; 22817900
329KLPRGRETMYSVVQKcurated25315811
344SQHRAHKSDSSREVPcurated22369663
346HRAHKSDSSREVPELPhosphoGRID 2.0;curated19779198; 22369663; 22817900; 24961812
376PRSVRRLSRTIKGVEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19366988; 22817900
448TIEARDLSLLGSHVRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900;
452RDLSLLGSHVRKATYcurated26040289; 22369663; 25315811
517SVDSRDTSLMERVTEcurated25315811
588DGVKNIPSFLSTDNIPhosphoGRID 2.0;curated21126336; 25315811
770KEPIMKRTTLVANTSPhosphoGRID 2.0;curated21126336; 22817900; 19547744
771EPIMKRTTLVANTSNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900; 19547744
776RTTLVANTSNMPVAAPhosphoPep 2.019060867
777TTLVANTSNMPVAARdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988
806RDQGKNVSMIADSSSPhosphoGRID 2.021126336
Sequence
(Fasta)		MAGAIENARK EIKRISLEDH AESEYGAIYS VSGPVVIAEN MIGCAMYELV KVGHDNLVGE 60
VIRIDGDKAT IQVYEETAGL TVGDPVLRTG KPLSVELGPG LMETIYDGIQ RPLKAIKEES 120
QSIYIPRGID TPALDRTIKW QFTPGKFQVG DHISGGDIYG SVFENSLISS HKILLPPRSR 180
GTITWIAPAG EYTLDEKILE VEFDGKKSDF TLYHTWPVRV PRPVTEKLSA DYPLLTGQRV 240
LDALFPCVQG GTTCIPGAFG CGKTVISQSL SKYSNSDAII YVGCFAKGTN VLMADGSIEC 300
IENIEVGNKV MGKDGRPREV IKLPRGRETM YSVVQKSQHR AHKSDSSREV PELLKFTCNA 360
THELVVRTPR SVRRLSRTIK GVEYFEVITF EMGQKKAPDG RIVELVKEVS KSYPISEGPE 420
RANELVESYR KASNKAYFEW TIEARDLSLL GSHVRKATYQ TYAPILYEND HFFDYMQKSK 480
FHLTIEGPKV LAYLLGLWIG DGLSDRATFS VDSRDTSLME RVTEYAEKLN LCAEYKDRKE 540
PQVAKTVNLY SKVVRGNGIR NNLNTENPLW DAIVGLGFLK DGVKNIPSFL STDNIGTRET 600
FLAGLIDSDG YVTDEHGIKA TIKTIHTSVR DGLVSLARSL GLVVSVNAEP AKVDMNGTKH 660
KISYAIYMSG GDVLLNVLSK CAGSKKFRPA PAAAFARECR GFYFELQELK EDDYYGITLS 720
DDSDHQFLLA NQVVVHNCGE RGNEMAEVLM EFPELYTEMS GTKEPIMKRT TLVANTSNMP 780
VAAREASIYT GITLAEYFRD QGKNVSMIAD SSSRWAEALR EISGRLGEMP ADQGFPAYLG 840
AKLASFYERA GKAVALGSPD RTGSVSIVAA VSPAGGDFSD PVTTATLGIT QVFWGLDKKL 900
AQRKHFPSIN TSVSYSKYTN VLNKFYDSNY PEFPVLRDRM KEILSNAEEL EQVVQLVGKS 960
ALSDSDKITL DVATLIKEDF LQQNGYSTYD AFCPIWKTFD MMRAFISYHD EAQKAVANGA 1020
NWSKLADSTG DVKHAVSSSK FFEPSRGEKE VHGEFEKLLS TMQERFAEST D 1072
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0068--Autocatalytic cleavage
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0238--DNA-binding
KW-0255--Endonuclease
KW-0375--Hydrogen ion transport
KW-0378--Hydrolase
KW-0404--Intron homing
KW-0406--Ion transport
KW-0472--Membrane
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0651--Protein splicing
KW-1185--Reference proteome
KW-0813--Transport
KW-0926--Vacuole
Interpro

IPR031686--ATP-synt_a_Xtn
IPR020003--ATPase_a/bsu_AS
IPR000793--ATPase_F1/V1/A1-cplx_a/bsu_C
IPR000194--ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR004100--ATPase_F1_a/bsu_N
IPR024034--ATPase_F1_bsu/V1_C
IPR028992--Hedgehog/Intein_dom
IPR003586--Hint_dom_C
IPR003587--Hint_dom_N
IPR007868--Hom_end_hint
IPR007869--Homing_endonuc_PI-Sce
IPR027434--Homing_endonucl
IPR006142--INTEIN
IPR030934--Intein_C
IPR004042--Intein_endonuc
IPR006141--Intein_N
IPR027417--P-loop_NTPase
PROSITE

PS00152--ATPASE_ALPHA_BETA
PS50818--INTEIN_C_TER
PS50819--INTEIN_ENDONUCLEASE
PS50817--INTEIN_N_TER
Pfam

PF00006--ATP-synt_ab
PF00306--ATP-synt_ab_C
PF02874--ATP-synt_ab_N
PF16886--ATP-synt_ab_Xtn
PF05204--Hom_end
PF05203--Hom_end_hint
Gene Ontology

GO:0012505--C:endomembrane system
GO:0000329--C:fungal-type vacuole membrane
GO:0000221--C:vacuolar proton-transporting V-type ATPase, V1 domain
GO:0005524--F:ATP binding
GO:0003677--F:DNA binding
GO:0004519--F:endonuclease activity
GO:0046961--F:proton-transporting ATPase activity, rotational mechanism
GO:0015991--P:ATP hydrolysis coupled proton transport
GO:0046034--P:ATP metabolic process
GO:0044267--P:cellular protein metabolic process
GO:0016539--P:intein-mediated protein splicing
GO:0006314--P:intron homing
GO:0007035--P:vacuolar acidification