dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006300
Uniprot Accession P17119; KAR3_YEAST; D6W4D9;
Genbank Protein ID NP_015467.1;
Genbank Nucleotide ID NM_001184238.1;
Protein Name Kinesin-like protein KAR3
Protein Synonyms/Alias Nuclear fusion protein;
Gene Name KAR3
Gene Synonyms/Alias YPR141C;P9659.16;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.
Phosphorylation Sites
 dbPAF PTMs: 16
PositionPeptidesSourceReferences ( PMIDs )
3-----MESLPRTPTKcurated22369663; 19823750
7-MESLPRTPTKGRSTPhosphoGRID 2.0;curated19520826; 26040289; 22369663; 22817900
14TPTKGRSTQHLSTPScurated19823750
18GRSTQHLSTPSPKNDPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 26040289; 19823750
19RSTQHLSTPSPKNDIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19060867; 19366988;
21TQHLSTPSPKNDILAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19779198;
37NGHKRRNTTTPPPKHcurated25315811
38GHKRRNTTTPPPKHTcurated25315811
39HKRRNTTTPPPKHTLcurated25315811
58RTDIHRHSLASQSRIPhosphoGRID 2.0;curated19779198; 22817900; 25315811
66LASQSRISMSPNRELcurated24961812
68SQSRISMSPNRELLKdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 24961812;
118ILFEKKATLDLLKDEPhosphoGRID 2.021126336
304AEKKKLLTEKRNAYEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19366988; 22817900
447KIFDQQDTNVDVFKEcurated25315811
570VTSCKLESEEMVEIIcurated22817900
Sequence
(Fasta)		MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ RTDIHRHSLA 60
SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE NVNELNRTQA ILFEKKATLD 120
LLKDELTETK EKINAVNLKF ETLREEKIKI EQQLNLKNNE LISIKEEFLS KKQFMNEGHE 180
IHLKQLAASN KKELKQMENE YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST 240
LQEMLNDVEQ KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 300
KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE YIKDTELGMK 360
ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL ENSDTSLINV NEFDDNSGVQ 420
SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK 480
TFTMLNPGDG IIPSTISHIF NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED 540
TSIGLKHEIR HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH 600
SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN KSLSCLGDVI 660
HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI SPSSSHINET LNSLRFASKV 720
NSTRLVSRK
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0131--Cell cycle
KW-0132--Cell division
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0415--Karyogamy
KW-0493--Microtubule
KW-0505--Motor protein
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
Interpro

IPR027640--Kinesin-like_fam
IPR019821--Kinesin_motor_CS
IPR001752--Kinesin_motor_dom
IPR027417--P-loop_NTPase
PROSITE

PS00411--KINESIN_MOTOR_1
PS50067--KINESIN_MOTOR_2
Pfam

PF00225--Kinesin
Gene Ontology

GO:0005881--C:cytoplasmic microtubule
GO:0005871--C:kinesin complex
GO:0005634--C:nucleus
GO:0005816--C:spindle pole body
GO:0005524--F:ATP binding
GO:0008569--F:ATP-dependent microtubule motor activity, minus-end-directed
GO:0008017--F:microtubule binding
GO:0046982--F:protein heterodimerization activity
GO:0051301--P:cell division
GO:0000742--P:karyogamy involved in conjugation with cellular fusion
GO:0007126--P:meiotic nuclear division
GO:0007067--P:mitotic nuclear division
GO:0007064--P:mitotic sister chromatid cohesion
GO:0000070--P:mitotic sister chromatid segregation
GO:0000743--P:nuclear migration involved in conjugation with cellular fusion
GO:0060236--P:regulation of mitotic spindle organization
GO:0051225--P:spindle assembly