dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006299
Uniprot Accession P17106; CBF1_YEAST; D6VWN1; P17623; Q6Q5N1;
Genbank Protein ID NP_012594.1;
Genbank Nucleotide ID NM_001181718.1;
Protein Name Centromere-binding protein 1
Protein Synonyms/Alias
Gene Name CBF1
Gene Synonyms/Alias CEP1;CP1;CPF1;YJR060W;J1730;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters. DNA-binding activity is enhanced by MET28. Required as an auxiliary factor for transcriptional activation of sulfur metabolism together with MET4 and MET28.
Phosphorylation Sites
 dbPAF PTMs: 21 (View all)
PositionPeptidesSourceReferences ( PMIDs )
19TEDEEIHSARKRGYNcurated24961812; 25315811
45QRDQGLLSQESNDGNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 20190278; 19684113;
48QGLLSQESNDGNIDSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 20190278;
55SNDGNIDSALLSEGAPhosphoGRID 2.0;curated19823750; 19795423; 22817900
59NIDSALLSEGATLKGcurated22369663; 24961812
63ALLSEGATLKGTQSQcurated22369663
69ATLKGTQSQYESGLTcurated22369663; 25315811
73GTQSQYESGLTSNKDcurated22369663
77QYESGLTSNKDEKGScurated22369663; 25315811
84SNKDEKGSDDEDASVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
130ANGEHKDSLNGERAIcurated19823750; 25315811; 20377248
138LNGERAITPSNEGVKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 21177495; 21126336;
140GERAITPSNEGVKPNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 17330950; 21126336; 19779198;
148NEGVKPNTSLEGMTSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19779198; 19060867; 26040289;
149EGVKPNTSLEGMTSSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
154NTSLEGMTSSPMESTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 19823750; 19795423;
155TSLEGMTSSPMESTQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 17330950; 21177495; 19779198;
156SLEGMTSSPMESTQQdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 17330950; 21177495;
160MTSSPMESTQQSKNDPhosphoGRID 2.0;curated19779198; 25315811; 20377248
161TSSPMESTQQSKNDMPhosphoGRID 2.019779198
Sequence
(Fasta)		MNSLANNNKL STEDEEIHSA RKRGYNEEQN YSEARKKQRD QGLLSQESND GNIDSALLSE 60
GATLKGTQSQ YESGLTSNKD EKGSDDEDAS VAEAAVAATV NYTDLIQGQE DSSDAHTSNQ 120
TNANGEHKDS LNGERAITPS NEGVKPNTSL EGMTSSPMES TQQSKNDMLI PLAEHDRGPE 180
HQQDDEDNDD ADIDLKKDIS MQPGRRGRKP TTLATTDEWK KQRKDSHKEV ERRRRENINT 240
AINVLSDLLP VRESSKAAIL ACAAEYIQKL KETDEANIEK WTLQKLLSEQ NASQLASANE 300
KLQEELGNAY KEIEYMKRVL RKEGIEYEDM HTHKKQENER KSTRSDNPHE A 352
Keyword

KW-0007--Acetylation
KW-0137--Centromere
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0238--DNA-binding
KW-0496--Mitochondrion
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR011598--bHLH_dom
PROSITE

PS50888--BHLH
Pfam

PF00010--HLH
Gene Ontology

GO:0089713--C:Cbf1-Met4-Met28 complex
GO:0000775--C:chromosome, centromeric region
GO:0000776--C:kinetochore
GO:0005739--C:mitochondrion
GO:0005634--C:nucleus
GO:0019237--F:centromeric DNA binding
GO:0001102--F:RNA polymerase II activating transcription factor binding
GO:0000978--F:RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001103--F:RNA polymerase II repressing transcription factor binding
GO:0001076--F:transcription factor activity, RNA polymerase II transcription factor binding
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001078--F:transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0006338--P:chromatin remodeling
GO:0007059--P:chromosome segregation
GO:0061427--P:negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:1900375--P:positive regulation of inositol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter
GO:1900478--P:positive regulation of sulfate assimilation by positive regulation of transcription from RNA polymerase II promoter
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0061432--P:regulation of transcription from RNA polymerase II promoter in response to methionine
GO:0006366--P:transcription from RNA polymerase II promoter