dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006289
Uniprot Accession P17065; SEC2_YEAST; D6W0S2;
Genbank Protein ID NP_014127.1;
Genbank Nucleotide ID NM_001183110.1;
Protein Name Rab guanine nucleotide exchange factor SEC2
Protein Synonyms/Alias GDP-GTP exchange factor SEC2;
Gene Name SEC2
Gene Synonyms/Alias YNL272C;N0641;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exhange activity on YPT32.
Phosphorylation Sites
 dbPAF PTMs: 32 (View all)
PositionPeptidesSourceReferences ( PMIDs )
134EILNKRLTEQLREKDcurated26040289
168LDNESTVTNNSNRYSPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 25315811
171ESTVTNNSNRYSTILPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 25315811
183TILSDSATSSSTSLNcurated22369663; 22817900; 19547744
184ILSDSATSSSTSLNKcurated22369663; 19795423; 25315811
185LSDSATSSSTSLNKVcurated22369663; 24961812; 25315811
186SDSATSSSTSLNKVPPHOSIDA;curated21081558; 22817900; 19795423; 25315811
187DSATSSSTSLNKVPTcurated22369663
188SATSSSTSLNKVPTScurated22369663
197NKVPTSYSLASQDIYdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663
200PTSYSLASQDIYSGIdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663
204SLASQDIYSGIVYSPdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
409KLTTQNITKEDLEKFdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900;
417KEDLEKFSEVTKHTKcurated22817900
515GPLVEDDSDEDQNDAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 21177495; 21126336;
524EDQNDAISVRLQDKAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 19684113; 19779198; 19060867;
542QDAKRPFSSSSAEESPhosphoGRID 2.0;curated20702584; 19823750
543DAKRPFSSSSAEESQcurated19823750
544AKRPFSSSSAEESQKdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 20702584; 22817900; 19823750
545KRPFSSSSAEESQKSdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 19823750; 19795423; 22369663;
Sequence
(Fasta)		MDASEEAKRV SIQVTSLSTQ LIESVDKQSH LEEQLNKSLK TIASQKAAIE NYNQLKEDYN 60
TLKRELSDRD DEVKRLREDI AKENELRTKA EEEADKLNKE VEDLTASLFD EANNMVADAR 120
KEKYAIEILN KRLTEQLREK DTLLDTLTLQ LKNLKKVMHS LDNESTVTNN SNRYSTILSD 180
SATSSSTSLN KVPTSYSLAS QDIYSGIVYS PSISSIRYDI SLYNEFLKFV AALPRCENIK 240
ATSTESKLIR RLVNDEIQPI LKIDNASGIG WLVKKTLLSL IIDGLVVVEP LSGVNATYQI 300
GYNSSSPAKQ ATSNMPKMFK FPLDSPPVAV HAACSFCGES RDDIIEHARM YILKTLHKTD 360
DGKEQVTNTY PLCHWCLLKL RQTCEIFAFL RSLKVGAWHL EKLTTQNITK EDLEKFSEVT 420
KHTKRDGRVS SQDKKTKRLS FMAGLGINSS TKNKPKMEIF SSETNAKPGQ PTTNIQRAWL 480
QLCKLRCILH WTHIGIWAVD DSISSKIGPL VEDDSDEDQN DAISVRLQDK ALWKQDAKRP 540
FSSSSAEESQ KSDAFDFESG DMENEITGES SSDESSSDGS STDNSTADSS SEDESSLADS 600
TTSSADSSSP ESIDNGEGDD TVTKDDKSSI KSANNNEENS DCGDKKGRSI IKKKAPQRKI 660
QKKKLLQDLD DLEEQFREES AIDQTEFENA ESNVKQNISS KRASSGDENS KKDNNEKTLK 720
TNLTIGDKTQ EQIGENSPSS GLHASSSNDD NFDDAQEQQ
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0968--Cytoplasmic vesicle
KW-0344--Guanine-nucleotide releasing factor
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
Interpro

IPR009449--Sec2_N
PROSITE
Pfam

PF06428--Sec2p
Gene Ontology

GO:0005935--C:cellular bud neck
GO:0005934--C:cellular bud tip
GO:0005829--C:cytosol
GO:0030133--C:transport vesicle
GO:0005085--F:guanyl-nucleotide exchange factor activity
GO:0070273--F:phosphatidylinositol-4-phosphate binding
GO:0006914--P:autophagy
GO:0006887--P:exocytosis
GO:0043547--P:positive regulation of GTPase activity
GO:0015031--P:protein transport