dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006246
Uniprot Accession P16862; PFKA2_YEAST; D6W030;
Genbank Protein ID NP_013932.1;
Genbank Nucleotide ID NM_001182712.1;
Protein Name ATP-dependent 6-phosphofructokinase subunit beta
Protein Synonyms/Alias ATP-dependent 6-phosphofructokinase;ATP-PFK;Phosphofructokinase 2;Phosphohexokinase;
Gene Name PFK2
Gene Synonyms/Alias YMR205C;YM8325.06C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Phosphorylation Sites
 dbPAF PTMs: 44 (View all)
PositionPeptidesSourceReferences ( PMIDs )
13PFVNGTSYCTVTAYScurated22817900
15VNGTSYCTVTAYSVQcurated22817900
20YCTVTAYSVQSYKAAcurated22817900
23VTAYSVQSYKAAIDFcurated22817900
31YKAAIDFYTKFLSLEcurated22817900
32KAAIDFYTKFLSLENcurated22817900
36DFYTKFLSLENRSSPPhosphoGRID 2.0;curated19779198; 26040406; 22369663; 25521595
41FLSLENRSSPDENSTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
42LSLENRSSPDENSTLdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated15665377; 18407956; 16381945; 23193290; 17330950; 19779198;
47RSSPDENSTLLSNDSPhosphoPep 2.0;curated19060867; 22817900
48SSPDENSTLLSNDSIcurated22817900
80AHLKELNSITKTQDWcurated22817900
82LKELNSITKTQDWRScurated22817900
148TSTKNAVSTKPTPPPPhosphoGRID 2.0;PhosphoPep 2.0;curated21126336; 19779198; 19060867; 22817900
149STKNAVSTKPTPPPAcurated24961812
152NAVSTKPTPPPAPEAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 21177495; 21126336; 19779198;
160PPPAPEASAESGLSSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated12872131; 15665377; 18407956; 16381945; 23193290; 19779198;
163APEASAESGLSSKVHdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
166ASAESGLSSKVHSYTdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 17330950; 21177495;
167SAESGLSSKVHSYTDdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 17330950; 21177495;
Sequence
(Fasta)		MTVTTPFVNG TSYCTVTAYS VQSYKAAIDF YTKFLSLENR SSPDENSTLL SNDSISLKIL 60
LRPDEKINKN VEAHLKELNS ITKTQDWRSH ATQSLVFNTS DILAVKDTLN AMNAPLQGYP 120
TELFPMQLYT LDPLGNVVGV TSTKNAVSTK PTPPPAPEAS AESGLSSKVH SYTDLAYRMK 180
TTDTYPSLPK PLNRPQKAIA VMTSGGDAPG MNSNVRAIVR SAIFKGCRAF VVMEGYEGLV 240
RGGPEYIKEF HWEDVRGWSA EGGTNIGTAR CMEFKKREGR LLGAQHLIEA GVDALIVCGG 300
DGSLTGADLF RSEWPSLIEE LLKTNRISNE QYERMKHLNI CGTVGSIDND MSTTDATIGA 360
YSALDRICKA IDYVEATANS HSRAFVVEVM GRNCGWLALL AGIATSADYI FIPEKPATSS 420
EWQDQMCDIV SKHRSRGKRT TIVVVAEGAI AADLTPISPS DVHKVLVDRL GLDTRITTLG 480
HVQRGGTAVA YDRILATLQG LEAVNAVLES TPDTPSPLIA VNENKIVRKP LMESVKLTKA 540
VAEAIQAKDF KRAMSLRDTE FIEHLNNFMA INSADHNEPK LPKDKRLKIA IVNVGAPAGG 600
INSAVYSMAT YCMSQGHRPY AIYNGWSGLA RHESVRSLNW KDMLGWQSRG GSEIGTNRVT 660
PEEADLGMIA YYFQKYEFDG LIIVGGFEAF ESLHQLERAR ESYPAFRIPM VLIPATLSNN 720
VPGTEYSLGS DTALNALMEY CDVVKQSASS TRGRAFVVDC QGGNSGYLAT YASLAVGAQV 780
SYVPEEGISL EQLSEDIEYL AQSFEKAEGR GRFGKLILKS TNASKALSAT KLAEVITAEA 840
DGRFDAKPAY PGHVQQGGLP SPIDRTRATR MAIKAVGFIK DNQAAIAEAR AAEENFNADD 900
KTISDTAAVV GVKGSHVVYN SIRQLYDYET EVSMRMPKVI HWQATRLIAD HLVGRKRVD 960
Keyword

KW-0002--3D-structure
KW-0021--Allosteric enzyme
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0324--Glycolysis
KW-0418--Kinase
KW-0460--Magnesium
KW-0472--Membrane
KW-0479--Metal-binding
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
Interpro

IPR009161--6-Pfructokinase_euk
IPR022953--ATP_PFK
IPR029068--Glyas_Bleomycin-R_OHBP_Dase
IPR015912--Phosphofructokinase_CS
IPR000023--Phosphofructokinase_dom
PROSITE

PS00433--PHOSPHOFRUCTOKINASE
Pfam

PF00365--PFK
Gene Ontology

GO:0005945--C:6-phosphofructokinase complex
GO:0005741--C:mitochondrial outer membrane
GO:0005739--C:mitochondrion
GO:0003872--F:6-phosphofructokinase activity
GO:0005524--F:ATP binding
GO:0046872--F:metal ion binding
GO:0003729--F:mRNA binding
GO:0006002--P:fructose 6-phosphate metabolic process
GO:0006096--P:glycolytic process
GO:0061615--P:glycolytic process through fructose-6-phosphate
GO:1902600--P:hydrogen ion transmembrane transport
GO:0015992--P:proton transport