dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006245
Uniprot Accession P16861; PFKA1_YEAST; D6VV20;
Genbank Protein ID NP_011756.1;
Genbank Nucleotide ID NM_001181369.1;
Protein Name ATP-dependent 6-phosphofructokinase subunit alpha
Protein Synonyms/Alias ATP-dependent 6-phosphofructokinase;ATP-PFK;Phosphofructokinase 1;Phosphohexokinase;
Gene Name PFK1
Gene Synonyms/Alias YGR240C;G8599;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Phosphorylation Sites
 dbPAF PTMs: 27 (View all)
PositionPeptidesSourceReferences ( PMIDs )
3-----MQSQDSCYGVdbPTM 3.0;PhosphoGRID 2.0;UniProt;curated18407956; 16381945; 23193290; 20190278; 21126336
6--MQSQDSCYGVAFRcurated19547744
50KFKHGENSLLSSGTScurated24961812
57SLLSSGTSQDSLREVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
92EATNKAQSQGALLKIcurated22369663
156DPMGNNITFSGLANAPhosphoGRID 2.021126336
166GLANATDSAPTSKDAdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22369663;
170ATDSAPTSKDAFLEAcurated22369663
178KDAFLEATSEDEIISdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 21126336; 20702584;
179DAFLEATSEDEIISRdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17330950; 18407956; 16381945; 23193290; 21177495;
185TSEDEIISRASSDASdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 19779198; 21081558;
188DEIISRASSDASDLLdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 20702584; 19779198;
189EIISRASSDASDLLRdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 17330950; 21177495; 20702584;
192SRASSDASDLLRQTLdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 20702584; 19779198;
201LLRQTLGSSQKKKKIcurated25315811
217VMTSGGDSPGMNAAVUniProt;curated19779198; 22817900
268EDVRGWLSEGGTLIGcurated22817900
450SKGRRNNTIIVAEGAUniProt;curated19779198; 22817900
494GHVQRGGTAVAHDRWcurated25315811
518VKAVLEFTPETPSPLPhosphoGRID 2.021126336
Sequence
(Fasta)		MQSQDSCYGV AFRSIITNDE ALFKKTIHFY HTLGFATVKD FNKFKHGENS LLSSGTSQDS 60
LREVWLESFK LSEVDASGFR IPQQEATNKA QSQGALLKIR LVMSAPIDET FDTNETATIT 120
YFSTDLNKIV EKFPKQAEKL SDTLVFLKDP MGNNITFSGL ANATDSAPTS KDAFLEATSE 180
DEIISRASSD ASDLLRQTLG SSQKKKKIAV MTSGGDSPGM NAAVRAVVRT GIHFGCDVFA 240
VYEGYEGLLR GGKYLKKMAW EDVRGWLSEG GTLIGTARSM EFRKREGRRQ AAGNLISQGI 300
DALVVCGGDG SLTGADLFRH EWPSLVDELV AEGRFTKEEV APYKNLSIVG LVGSIDNDMS 360
GTDSTIGAYS ALERICEMVD YIDATAKSHS RAFVVEVMGR HCGWLALMAG IATGADYIFI 420
PERAVPHGKW QDELKEVCQR HRSKGRRNNT IIVAEGALDD QLNPVTANDV KDALIELGLD 480
TKVTILGHVQ RGGTAVAHDR WLATLQGVDA VKAVLEFTPE TPSPLIGILE NKIIRMPLVE 540
SVKLTKSVAT AIENKDFDKA ISLRDTEFIE LYENFLSTTV KDDGSELLPV SDRLNIGIVH 600
VGAPSAALNA ATRAATLYCL SHGHKPYAIM NGFSGLIQTG EVKELSWIDV ENWHNLGGSE 660
IGTNRSVASE DLGTIAYYFQ KNKLDGLIIL GGFEGFRSLK QLRDGRTQHP IFNIPMCLIP 720
ATVSNNVPGT EYSLGVDTCL NALVNYTDDI KQSASATRRR VFVCEVQGGH SGYIASFTGL 780
ITGAVSVYTP EKKIDLASIR EDITLLKENF RHDKGENRNG KLLVRNEQAS SVYSTQLLAD 840
IISEASKGKF GVRTAIPGHV QQGGVPSSKD RVTASRFAVK CIKFIEQWNK KNEASPNTDA 900
KVLRFKFDTH GEKVPTVEHE DDSAAVICVN GSHVSFKPIA NLWENETNVE LRKGFEVHWA 960
EYNKIGDILS GRLKLRAEVA ALAAENK 988
Keyword

KW-0002--3D-structure
KW-0021--Allosteric enzyme
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0324--Glycolysis
KW-0418--Kinase
KW-0460--Magnesium
KW-0472--Membrane
KW-0479--Metal-binding
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
Interpro

IPR009161--6-Pfructokinase_euk
IPR022953--ATP_PFK
IPR015912--Phosphofructokinase_CS
IPR000023--Phosphofructokinase_dom
PROSITE

PS00433--PHOSPHOFRUCTOKINASE
Pfam

PF00365--PFK
Gene Ontology

GO:0005945--C:6-phosphofructokinase complex
GO:0005741--C:mitochondrial outer membrane
GO:0005739--C:mitochondrion
GO:0003872--F:6-phosphofructokinase activity
GO:0005524--F:ATP binding
GO:0046872--F:metal ion binding
GO:0006002--P:fructose 6-phosphate metabolic process
GO:0006096--P:glycolytic process
GO:0061615--P:glycolytic process through fructose-6-phosphate
GO:1902600--P:hydrogen ion transmembrane transport
GO:0015992--P:proton transport