dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006229
Uniprot Accession P16603; NCPR_YEAST; D3DKZ0;
Genbank Protein ID NP_011908.1;
Genbank Nucleotide ID NM_001179172.1;
Protein Name NADPH--cytochrome P450 reductase
Protein Synonyms/Alias
Gene Name NCP1
Gene Synonyms/Alias CPR1;NCPR1;PRD1;YHR042W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis. Has NADPH-dependent ferrireductase activity on the plasma membrane.
Phosphorylation Sites
 dbPAF PTMs: 8
PositionPeptidesSourceReferences ( PMIDs )
41MSDDGDITAVSSGNRcurated24961812; 20377248
44DGDITAVSSGNRDIAcurated24961812; 25315811
61VTENNKNYLVLYASQcurated26040406
67NYLVLYASQTGTAEDcurated26040406
69LVLYASQTGTAEDYAcurated26040406
75QTGTAEDYAKKFSKEcurated26040406
172KKAEKHLSAAGAIRLPhosphoGRID 2.0;curated19779198; 22817900; 24961812; 25315811; 19547744
447YYSISSSSLSEKQTVcurated22369663
Sequence
(Fasta)		MPFGIDNTDF TVLAGLVLAV LLYVKRNSIK ELLMSDDGDI TAVSSGNRDI AQVVTENNKN 60
YLVLYASQTG TAEDYAKKFS KELVAKFNLN VMCADVENYD FESLNDVPVI VSIFISTYGE 120
GDFPDGAVNF EDFICNAEAG ALSNLRYNMF GLGNSTYEFF NGAAKKAEKH LSAAGAIRLG 180
KLGEADDGAG TTDEDYMAWK DSILEVLKDE LHLDEQEAKF TSQFQYTVLN EITDSMSLGE 240
PSAHYLPSHQ LNRNADGIQL GPFDLSQPYI APIVKSRELF SSNDRNCIHS EFDLSGSNIK 300
YSTGDHLAVW PSNPLEKVEQ FLSIFNLDPE TIFDLKPLDP TVKVPFPTPT TIGAAIKHYL 360
EITGPVSRQL FSSLIQFAPN ADVKEKLTLL SKDKDQFAVE ITSKYFNIAD ALKYLSDGAK 420
WDTVPMQFLV ESVPQMTPRY YSISSSSLSE KQTVHVTSIV ENFPNPELPD APPVVGVTTN 480
LLRNIQLAQN NVNIAETNLP VHYDLNGPRK LFANYKLPVH VRRSNFRLPS NPSTPVIMIG 540
PGTGVAPFRG FIRERVAFLE SQKKGGNNVS LGKHILFYGS RNTDDFLYQD EWPEYAKKLD 600
GSFEMVVAHS RLPNTKKVYV QDKLKDYEDQ VFEMINNGAF IYVCGDAKGM AKGVSTALVG 660
ILSRGKSITT DEATELIKML KTSGRYQEDV W 692
Keyword

KW-0002--3D-structure
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0256--Endoplasmic reticulum
KW-0274--FAD
KW-0285--Flavoprotein
KW-0288--FMN
KW-1017--Isopeptide bond
KW-0444--Lipid biosynthesis
KW-0443--Lipid metabolism
KW-0472--Membrane
KW-0492--Microsome
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0521--NADP
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0752--Steroid biosynthesis
KW-0753--Steroid metabolism
KW-0756--Sterol biosynthesis
KW-1207--Sterol metabolism
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0832--Ubl conjugation
Interpro

IPR003097--FAD-binding_1
IPR017927--Fd_Rdtase_FAD-bd
IPR001094--Flavdoxin
IPR008254--Flavodoxin/NO_synth
IPR001709--Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039--Flavoprotein-like
IPR023173--NADPH_Cyt_P450_Rdtase_dom3
IPR001433--OxRdtase_FAD/NAD-bd
IPR023208--P450R
IPR017938--Riboflavin_synthase-like_b-brl
PROSITE

PS51384--FAD_FR
PS50902--FLAVODOXIN_LIKE
Pfam

PF00667--FAD_binding_1
PF00258--Flavodoxin_1
PF00175--NAD_binding_1
Gene Ontology

GO:0005829--C:cytosol
GO:0005789--C:endoplasmic reticulum membrane
GO:0016021--C:integral component of membrane
GO:0005741--C:mitochondrial outer membrane
GO:0005886--C:plasma membrane
GO:0009055--F:electron carrier activity
GO:0010181--F:FMN binding
GO:0005506--F:iron ion binding
GO:0003958--F:NADPH-hemoprotein reductase activity
GO:0016709--F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0006696--P:ergosterol biosynthetic process