dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006217
Uniprot Accession P16521; EF3A_YEAST; D6VYP7; O93815; Q06558;
Genbank Protein ID NP_013350.1;
Genbank Nucleotide ID NM_001182136.1;
Protein Name Elongation factor 3A
Protein Synonyms/Alias
Gene Name YEF3
Gene Synonyms/Alias EFC1;TEF3;YEF3A;YLR249W;L9672.5;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.
Phosphorylation Sites
 dbPAF PTMs: 33 (View all)
PositionPeptidesSourceReferences ( PMIDs )
4----MSDSQQSIKVLcurated26040406
7-MSDSQQSIKVLEELcurated26040406
19EELFQKLSVATADNRPhosphoGRID 2.0;curated19779198; 22817900; 19547744
35EIASEVASFLNGNIIcurated22369663; 24961812; 23607784
176PELIPVLSETMWDTKcurated22369663
178LIPVLSETMWDTKKEcurated22369663
266ETGIKRKSAVIIDNMcurated22817900; 19547744
638KCPAAKAYEELSNTDcurated25521595
642AKAYEELSNTDLEFKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21126336; 19779198;
644AYEELSNTDLEFKFPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 26040406;
679MEFQYPGTSKPQITDcurated25315811
680EFQYPGTSKPQITDIcurated25315811
708GPNGAGKSTLINVLTcurated26040289
721LTGELLPTSGEVYTHcurated26040289
726LPTSGEVYTHENCRIcurated26040289
727PTSGEVYTHENCRIAcurated26040289
735HENCRIAYIKQHAFAcurated22817900
751IESHLDKTPSEYIQWPhosphoGRID 2.0;curated19779198; 22817900
753SHLDKTPSEYIQWRFPhosphoGRID 2.0;curated19779198; 22817900
762YIQWRFQTGEDRETMPhosphoGRID 2.0;curated17287358
Sequence
(Fasta)		MSDSQQSIKV LEELFQKLSV ATADNRHEIA SEVASFLNGN IIEHDVPEHF FGELAKGIKD 60
KKTAANAMQA VAHIANQSNL SPSVEPYIVQ LVPAICTNAG NKDKEIQSVA SETLISIVNA 120
VNPVAIKALL PHLTNAIVET NKWQEKIAIL AAISAMVDAA KDQVALRMPE LIPVLSETMW 180
DTKKEVKAAA TAAMTKATET VDNKDIERFI PSLIQCIADP TEVPETVHLL GATTFVAEVT 240
PATLSIMVPL LSRGLNERET GIKRKSAVII DNMCKLVEDP QVIAPFLGKL LPGLKSNFAT 300
IADPEAREVT LRALKTLRRV GNVGEDDAIP EVSHAGDVST TLQVVNELLK DETVAPRFKI 360
VVEYIAAIGA DLIDERIIDQ QAWFTHITPY MTIFLHEKKA KDILDEFRKR AVDNIPVGPN 420
FDDEEDEGED LCNCEFSLAY GAKILLNKTQ LRLKRARRYG ICGPNGCGKS TLMRAIANGQ 480
VDGFPTQEEC RTVYVEHDID GTHSDTSVLD FVFESGVGTK EAIKDKLIEF GFTDEMIAMP 540
ISALSGGWKM KLALARAVLR NADILLLDEP TNHLDTVNVA WLVNYLNTCG ITSITISHDS 600
VFLDNVCEYI INYEGLKLRK YKGNFTEFVK KCPAAKAYEE LSNTDLEFKF PEPGYLEGVK 660
TKQKAIVKVT NMEFQYPGTS KPQITDINFQ CSLSSRIAVI GPNGAGKSTL INVLTGELLP 720
TSGEVYTHEN CRIAYIKQHA FAHIESHLDK TPSEYIQWRF QTGEDRETMD RANRQINEND 780
AEAMNKIFKI EGTPRRIAGI HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWI 840
PRGELVESHS KMVAEVDMKE ALASGQFRPL TRKEIEEHCS MLGLDPEIVS HSRIRGLSGG 900
QKVKLVLAAG TWQRPHLIVL DEPTNYLDRD SLGALSKALK EFEGGVIIIT HSAEFTKNLT 960
EEVWAVKDGR MTPSGHNWVS GQGAGPRIEK KEDEEDKFDA MGNKIAGGKK KKKLSSAELR 1020
KKKKERMKKK KELGDAYVSS DEEF 1045
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0251--Elongation factor
KW-0488--Methylation
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0677--Repeat
KW-0694--RNA-binding
KW-0699--rRNA-binding
Interpro

IPR003593--AAA+_ATPase
IPR003439--ABC_transporter-like
IPR017871--ABC_transporter_CS
IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR015688--Elongation_fac_3
IPR021133--HEAT_type_2
IPR027417--P-loop_NTPase
PROSITE

PS00211--ABC_TRANSPORTER_1
PS50893--ABC_TRANSPORTER_2
PS50077--HEAT_REPEAT
Pfam

PF00005--ABC_tran
Gene Ontology

GO:0010494--C:cytoplasmic stress granule
GO:0022626--C:cytosolic ribosome
GO:0005840--C:ribosome
GO:0005524--F:ATP binding
GO:0016887--F:ATPase activity
GO:0019843--F:rRNA binding
GO:0003746--F:translation elongation factor activity
GO:0006414--P:translational elongation
GO:0006415--P:translational termination