dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006147
Uniprot Accession P16140; VATB_YEAST; D6VQC4; P32123;
Genbank Protein ID NP_009685.3;
Genbank Nucleotide ID NM_001178475.3;
Protein Name V-type proton ATPase subunit B
Protein Synonyms/Alias
Gene Name VMA2
Gene Synonyms/Alias VAT2;YBR127C;YBR1002;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
4----MVLSDKELFAIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 21126336; 20702584;
83IVQVFEGTSGIDVKKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
84VQVFEGTSGIDVKKTdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
92GIDVKKTTVEFTGEScurated22817900; 19547744
137DYLDINGSPINPYARdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040289;
370QLHNKGIYPPINVLPcurated22817900
378PPINVLPSLSRLMKSdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900
380INVLPSLSRLMKSAIcurated22817900
385SLSRLMKSAIGEGMTcurated25315811
407SNQLYAKYAIGKDAAPhosphoGRID 2.0;curated19779198; 22817900
426VVGEEALSIEDKLSLcurated22369663
463ESLDQAWSLLRIYPKcurated22369663; 23607784
477KEMLNRISPKILDEFcurated22369663
485PKILDEFYDRARDDAPhosphoGRID 2.0;curated19823750; 19795423
501EDEEDPDTRSSGKKKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21126336; 20702584;
503EEDPDTRSSGKKKDAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17563356; 18407956; 16381945; 23193290; 21177495;
504EDPDTRSSGKKKDASdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 21177495; 21081558;
511SGKKKDASQEESLI-dbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 17563356; 18407956; 16381945; 23193290; 21126336;
515KDASQEESLI-----dbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 20702584;
Sequence
(Fasta)		MVLSDKELFA INKKAVEQGF NVKPRLNYNT VSGVNGPLVI LEKVKFPRYN EIVNLTLPDG 60
TVRQGQVLEI RGDRAIVQVF EGTSGIDVKK TTVEFTGESL RIPVSEDMLG RIFDGSGRPI 120
DNGPKVFAED YLDINGSPIN PYARIYPEEM ISTGVSAIDT MNSIARGQKI PIFSASGLPH 180
NEIAAQICRQ AGLVRPTKDV HDGHEENFSI VFAAMGVNLE TARFFKQDFE ENGSLERTSL 240
FLNLANDPTI ERIITPRLAL TTAEYLAYQT ERHVLTILTD MSSYADALRE VSAAREEVPG 300
RRGYPGYMYT DLSTIYERAG RVEGRNGSIT QIPILTMPND DITHPIPDLT GYITEGQIFV 360
DRQLHNKGIY PPINVLPSLS RLMKSAIGEG MTRKDHGDVS NQLYAKYAIG KDAAAMKAVV 420
GEEALSIEDK LSLEFLEKFE KTFITQGAYE DRTVFESLDQ AWSLLRIYPK EMLNRISPKI 480
LDEFYDRARD DADEDEEDPD TRSSGKKKDA SQEESLI 518
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0375--Hydrogen ion transport
KW-0378--Hydrolase
KW-0406--Ion transport
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0813--Transport
Interpro

IPR020003--ATPase_a/bsu_AS
IPR000793--ATPase_F1/V1/A1-cplx_a/bsu_C
IPR000194--ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR004100--ATPase_F1_a/bsu_N
IPR005723--ATPase_V1-cplx_bsu
IPR027417--P-loop_NTPase
IPR022879--V-ATPase_su_B/beta
PROSITE

PS00152--ATPASE_ALPHA_BETA
Pfam

PF00006--ATP-synt_ab
PF00306--ATP-synt_ab_C
PF02874--ATP-synt_ab_N
Gene Ontology

GO:0000329--C:fungal-type vacuole membrane
GO:0000221--C:vacuolar proton-transporting V-type ATPase, V1 domain
GO:1990816--C:vacuole-mitochondrion membrane contact site
GO:0005524--F:ATP binding
GO:0046961--F:proton-transporting ATPase activity, rotational mechanism
GO:0015991--P:ATP hydrolysis coupled proton transport
GO:0046034--P:ATP metabolic process
GO:0006874--P:cellular calcium ion homeostasis
GO:1902906--P:proteasome storage granule assembly
GO:0055085--P:transmembrane transport
GO:0007035--P:vacuolar acidification