dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0006115
Uniprot Accession P15938; PRP16_YEAST; D6VXE6;
Genbank Protein ID NP_013012.3;
Genbank Nucleotide ID NM_001179876.3;
Protein Name Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Protein Synonyms/Alias
Gene Name PRP16
Gene Synonyms/Alias YKR086W;YKR406;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Influences the fidelity of branchpoint recognition in yeast splicing. This is RNA-dependent ATPase which is essential for viability. It may mediate one of the many ATP-requiring steps of spliceosome assembly and that accuracy of branchpoint recognition may be coupled to ATP binding and/or hydrolysis.
Phosphorylation Sites
dbPAF PTMs: 3
PositionPeptidesSourceReferences ( PMIDs )
108DNEDELDSPVQKKPAcurated25315811
143SPTVKDPSPNSEQQTcurated24961812; 25315811
895SQKIPVISSGKDWDIPhosphoGRID 2.0;curated21177495; 22817900
Sequence
(Fasta)
MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA LTKLSSNNPI 60
IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD DNEDELDSPV QKKPAPTRTL 120
FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT QNGHAETKDY EPTRSEVVEE DREWYDNDDD 180
YGNLVPEPLS ELPEEAKLLP VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN 240
KVPTSIIIGS ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD 300
NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL PVFRCRSQLL 360
SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI VVTQPRRVAA ISVAKRVAME 420
MQVPLGKEVG YSIRFEDVTD SECTKLKFVT DGILLRETLL DDTLDKYSCV IIDEAHERSL 480
NTDILLGFFK ILLARRRDLK LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP 540
VQDYVEAAVS QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF 600
EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI RYVIDCGYSK 660
LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR LYTEDTFKED MYLQTIPEIQ 720
RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM 780
AKFPLQPSLS KILLIAVRNG CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS 840
DHLTLLNVFE QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW 900
DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY VVYHELLMTS 960
KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF GEHYEHTLDK VEDDIDINIR 1020
RCKDMRDSVI QELKMTDNSN KEDKKQKTKK QNILNGKENS MKPFKRRKPF F 1072
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0347--Helicase
KW-0378--Hydrolase
KW-0507--mRNA processing
KW-0508--mRNA splicing
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome

Interpro

IPR011545--DEAD/DEAH_box_helicase_dom
IPR002464--DNA/RNA_helicase_DEAH_CS
IPR011709--DUF1605
IPR007502--Helicase-assoc_dom
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR027417--P-loop_NTPase

PROSITE

PS00690--DEAH_ATP_HELICASE
PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER

Pfam

PF00270--DEAD
PF04408--HA2
PF00271--Helicase_C
PF07717--OB_NTP_bind

Gene Ontology

GO:0005737--C:cytoplasm
GO:0071007--C:U2-type catalytic step 2 spliceosome
GO:0005524--F:ATP binding
GO:0004004--F:ATP-dependent RNA helicase activity
GO:0044822--F:poly(A) RNA binding
GO:0008186--F:RNA-dependent ATPase activity
GO:0000350--P:generation of catalytic spliceosome for second transesterification step
GO:0000398--P:mRNA splicing, via spliceosome
GO:0000378--P:RNA exon ligation