dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006107
Uniprot Accession P15891; ABP1_YEAST; D6VR88;
Genbank Protein ID NP_010012.1;
Genbank Nucleotide ID NM_001178794.1;
Protein Name Actin-binding protein
Protein Synonyms/Alias
Gene Name ABP1
Gene Synonyms/Alias YCR088W;YCR88W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Regulates ARP2/3 complex-mediated actin assembly. Recruits ARP2/3 complex to sides of preexisting actin filaments, which may promote nucleation or stabilization of filament branches. Binds to actin filaments, but not actin monomers. Actin binding is required for ARP2/3 complex activation. May also have a role in linking the actin cytoskeleton to endocytosis. recruits components of the endocytotic machinery to cortical actin patches, known sites of endocytosis.
Phosphorylation Sites
 dbPAF PTMs: 41 (View all)
PositionPeptidesSourceReferences ( PMIDs )
26YLKIVRGSDPDTTWLPhosphoGRID 2.0;curated21177495; 22817900
30VRGSDPDTTWLIISPPhosphoGRID 2.0;curated21177495; 22817900
31RGSDPDTTWLIISPNPhosphoGRID 2.0;curated21177495; 22817900
144NAAGARYSIQTSSKQcurated22369663; 24961812
156SKQQGKASTPPVKKSPhosphoGRID 2.0;curated17563356; 25315811
157KQQGKASTPPVKKSFdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 21081558;
163STPPVKKSFTPSKSPPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 21126336; 19779198; 19060867; 22817900; 23825934;
165PPVKKSFTPSKSPAPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 15665377; 17287358; 17563356; 18407956; 16381945;
167VKKSFTPSKSPAPVSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 16381945; 23193290; 17330950; 21177495; 19823750;
169KSFTPSKSPAPVSKKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 15665377; 17287358; 17563356; 18407956; 16381945;
174SKSPAPVSKKEPVKTPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19823750; 19795423; 19060867; 22817900; 23825934;
181SKKEPVKTPSPAPAAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17287358; 17330950; 17563356; 18407956; 16381945;
183KEPVKTPSPAPAAKIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17287358; 17330950; 17563356; 18407956; 16381945;
191PAPAAKISSRVNDNNPhosphoPep 2.0;curated19060867; 20377248
192APAAKISSRVNDNNDPhosphoPep 2.0;curated19060867; 22369663; 24961812; 20377248
223APLKPNQSSYKPIGKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
224PLKPNQSSYKPIGKIPhosphoPep 2.0;curated19060867; 26040406; 25315811
225LKPNQSSYKPIGKIDdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040289
253PRLVQKPTAAGSKIDcurated22817900
257QKPTAAGSKIDPSSDcurated22369663
Sequence
(Fasta)		MALEPIDYTT HSREIDAEYL KIVRGSDPDT TWLIISPNAK KEYEPESTGS SFHDFLQLFD 60
ETKVQYGLAR VSPPGSDVEK IIIIGWCPDS APLKTRASFA ANFAAVANNL FKGYHVQVTA 120
RDEDDLDENE LLMKISNAAG ARYSIQTSSK QQGKASTPPV KKSFTPSKSP APVSKKEPVK 180
TPSPAPAAKI SSRVNDNNDD DDWNEPELKE RDFDQAPLKP NQSSYKPIGK IDLQKVIAEE 240
KAKEDPRLVQ KPTAAGSKID PSSDIANLKN ESKLKRDSEF NSFLGTTKPP SMTESSLKND 300
DDKVIKGFRN EKSPAQLWAE RKAKQNSGNA ETKAEAPKPE VPEDEPEGEP DVKDLKSKFE 360
GLAASEKEEE EMENKFAPPP KKSEPTIISP KPFSKPQEPV KAEEAEQPKT DYKKIGNPLP 420
GMHIEADNEE EPEENDDDWD DDEDEAAQPP LPSRNVASGA PVQKEEPEQE EIAPSLPSRN 480
SIPAPKQEEA PEQAPEEEIE EEAEEAAPQL PSRSSAAPPP PPRRATPEKK PKENPWATAE 540
YDYDAAEDNE LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN 593
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0009--Actin-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0903--Direct protein sequencing
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0728--SH3 domain
Interpro

IPR002108--ADF-H
IPR029006--ADF-H/Gelsolin-like_dom
IPR001452--SH3_domain
PROSITE

PS51263--ADF_H
PS50002--SH3
Pfam

PF00241--Cofilin_ADF
PF00018--SH3_1
Gene Ontology

GO:0030479--C:actin cortical patch
GO:0005938--C:cell cortex
GO:0051015--F:actin filament binding
GO:0000147--P:actin cortical patch assembly
GO:0051016--P:barbed-end actin filament capping
GO:0008104--P:protein localization